Effect of porosity on the colonization of digital light-processed 3D hydrogel constructs toward the development of a functional intestinal model
- Author
- Anna Szabó, Elly De Vlieghere (UGent) , Pedro F. Costa, Indi Geurs (UGent) , Koen Dewettinck (UGent) , Laure Maes (UGent) , Debby Laukens (UGent) and Sandra Van Vlierberghe (UGent)
- Organization
- Project
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- Fish-AI (Developing an Artificial Intestine for the sutainable farming of healthy fish)
- Cryo-scanning electron microscope (Cryo-SEM)
- Abstract
- With the rapid increase of the number of patients with gastrointestinal diseases in modern society, the need for the development of physiologically relevant in vitro intestinal models is key to improve the understanding of intestinal dysfunctions. This involves the development of a scaffold material exhibiting physiological stiffness and anatomical mimicry of the intestinal architecture. The current work focuses on evaluating the scaffold micromorphology of gelatin-methacryloyl-aminoethyl-methacrylate-based nonporous and porous intestinal 3D, intestine-like constructs, fabricated via digital light processing, on the cellular response. To this end, Caco-2 intestinal cells were utilized in combination with the constructs. Both porous and nonporous constructs promoted cell growth and differentiation toward enterocyte-like cells (VIL1, ALPI, SI, and OCLD expression showed via qPCR, ZO-1 via immunostaining). The porous constructs outperformed the nonporous ones regarding cell seeding efficiency and growth rate, confirmed by MTS assay, live/dead staining, and TEER measurements, due to the presence of surface roughness.
- Keywords
- IN-VITRO, MECHANICAL-PROPERTIES, STEM-CELL, CACO-2, SCAFFOLDS, PERMEABILITIES, TRANSPORT, SYSTEMS, MATRIX
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HX9G1P62CCTYF9R45MG3H8AE
- MLA
- Szabó, Anna, et al. “Effect of Porosity on the Colonization of Digital Light-Processed 3D Hydrogel Constructs toward the Development of a Functional Intestinal Model.” BIOMACROMOLECULES, vol. 25, no. 5, 2024, pp. 2863–74, doi:10.1021/acs.biomac.4c00019.
- APA
- Szabó, A., De Vlieghere, E., Costa, P. F., Geurs, I., Dewettinck, K., Maes, L., … Van Vlierberghe, S. (2024). Effect of porosity on the colonization of digital light-processed 3D hydrogel constructs toward the development of a functional intestinal model. BIOMACROMOLECULES, 25(5), 2863–2874. https://doi.org/10.1021/acs.biomac.4c00019
- Chicago author-date
- Szabó, Anna, Elly De Vlieghere, Pedro F. Costa, Indi Geurs, Koen Dewettinck, Laure Maes, Debby Laukens, and Sandra Van Vlierberghe. 2024. “Effect of Porosity on the Colonization of Digital Light-Processed 3D Hydrogel Constructs toward the Development of a Functional Intestinal Model.” BIOMACROMOLECULES 25 (5): 2863–74. https://doi.org/10.1021/acs.biomac.4c00019.
- Chicago author-date (all authors)
- Szabó, Anna, Elly De Vlieghere, Pedro F. Costa, Indi Geurs, Koen Dewettinck, Laure Maes, Debby Laukens, and Sandra Van Vlierberghe. 2024. “Effect of Porosity on the Colonization of Digital Light-Processed 3D Hydrogel Constructs toward the Development of a Functional Intestinal Model.” BIOMACROMOLECULES 25 (5): 2863–2874. doi:10.1021/acs.biomac.4c00019.
- Vancouver
- 1.Szabó A, De Vlieghere E, Costa PF, Geurs I, Dewettinck K, Maes L, et al. Effect of porosity on the colonization of digital light-processed 3D hydrogel constructs toward the development of a functional intestinal model. BIOMACROMOLECULES. 2024;25(5):2863–74.
- IEEE
- [1]A. Szabó et al., “Effect of porosity on the colonization of digital light-processed 3D hydrogel constructs toward the development of a functional intestinal model,” BIOMACROMOLECULES, vol. 25, no. 5, pp. 2863–2874, 2024.
@article{01HX9G1P62CCTYF9R45MG3H8AE,
abstract = {{With the rapid increase of the number of patients with gastrointestinal diseases in modern society, the need for the development of physiologically relevant in vitro intestinal models is key to improve the understanding of intestinal dysfunctions. This involves the development of a scaffold material exhibiting physiological stiffness and anatomical mimicry of the intestinal architecture. The current work focuses on evaluating the scaffold micromorphology of gelatin-methacryloyl-aminoethyl-methacrylate-based nonporous and porous intestinal 3D, intestine-like constructs, fabricated via digital light processing, on the cellular response. To this end, Caco-2 intestinal cells were utilized in combination with the constructs. Both porous and nonporous constructs promoted cell growth and differentiation toward enterocyte-like cells (VIL1, ALPI, SI, and OCLD expression showed via qPCR, ZO-1 via immunostaining). The porous constructs outperformed the nonporous ones regarding cell seeding efficiency and growth rate, confirmed by MTS assay, live/dead staining, and TEER measurements, due to the presence of surface roughness.}},
author = {{Szabó, Anna and De Vlieghere, Elly and Costa, Pedro F. and Geurs, Indi and Dewettinck, Koen and Maes, Laure and Laukens, Debby and Van Vlierberghe, Sandra}},
issn = {{1525-7797}},
journal = {{BIOMACROMOLECULES}},
keywords = {{IN-VITRO,MECHANICAL-PROPERTIES,STEM-CELL,CACO-2,SCAFFOLDS,PERMEABILITIES,TRANSPORT,SYSTEMS,MATRIX}},
language = {{eng}},
number = {{5}},
pages = {{2863--2874}},
title = {{Effect of porosity on the colonization of digital light-processed 3D hydrogel constructs toward the development of a functional intestinal model}},
url = {{http://doi.org/10.1021/acs.biomac.4c00019}},
volume = {{25}},
year = {{2024}},
}
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