Advanced search
2 files | 2.06 MB Add to list

MicroRNA-146b negatively affects bovine embryo development and quality

Krishna Chaitanya Pavani (UGent) , Guan XueFeng (UGent) , Jayendra Chunduru, Tim Meese (UGent) , Luc Peelman (UGent) , Filip Van Nieuwerburgh (UGent) , Dieter Deforce (UGent) , An Hendrix (UGent) , Kelly Tilleman (UGent) , Ann Van Soom (UGent) , et al.
(2024) REPRODUCTION. 167(2).
Author
Organization
Project
Abstract
MicroRNAs (miRNAs), which can be carried inside extracellular vesicles (EVs), play a crucial role in regulating embryo development up to the blastocyst stage. Yet, the molecular mechanisms underlying blastocyst development and quality are largely unknown. Recently, our group identified 69 differentially expressed miRNAs in extracellular vesicles (EVs) isolated from culture medium conditioned by bovine embryos that either developed to the blastocyst stage or did not (non-blastocysts). We found miR-146b to be more abundant in the EVs derived from media conditioned by non-blastocyst embryos. Using RT-qPCR, we here confirmed the upregulation of miR-146b in non-blastocyst (arrested at two- to four-cell and morula stage) embryos compared to blastocysts (P < 0.005), which coincides with the upregulation of miR-146b in EVs derived from the medium of these non-blastocysts. To evaluate a functional effect, bovine embryo culture media were supplemented with miR-146b mimics, resulting in significantly decreased embryo quality, with lower blastocyst rates at day 7 and lower total cell numbers, while the opposite was found after supplementation with miR-146b inhibitors, which resulted in reduced apoptosis rates (P < 0.01). Transcriptomic analysis of embryos treated with miR-146b mimics or inhibitors showed differential expression (P < 0.01) of genes associated with apoptosis, cell differentiation, and the RNA Pol II transcription complex, including WDR36, MBNL2, ERCC6l2, PYGO1, and SNIP1. Overall, miR-146b is overexpressed in non-blastocyst embryos and in EVs secreted by these embryos, and it regulates genes involved in embryo development and apoptosis, resulting in decreased embryo quality.
Keywords
CULTURE-MEDIA, PREIMPLANTATION EMBRYOS, EXTRACELLULAR VESICLES, EXPRESSION, COMMUNICATION, CANCER, RNA

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.24 MB
  • Krishna Chaitanya Pavani et al Accepted Manuscript.pdf
    • full text (Accepted manuscript)
    • |
    • open access
    • |
    • PDF
    • |
    • 821.03 KB

Citation

Please use this url to cite or link to this publication:

MLA
Pavani, Krishna Chaitanya, et al. “MicroRNA-146b Negatively Affects Bovine Embryo Development and Quality.” REPRODUCTION, vol. 167, no. 2, 2024, doi:10.1530/REP-23-0155.
APA
Pavani, K. C., XueFeng, G., Chunduru, J., Meese, T., Peelman, L., Van Nieuwerburgh, F., … Smits, K. (2024). MicroRNA-146b negatively affects bovine embryo development and quality. REPRODUCTION, 167(2). https://doi.org/10.1530/REP-23-0155
Chicago author-date
Pavani, Krishna Chaitanya, Guan XueFeng, Jayendra Chunduru, Tim Meese, Luc Peelman, Filip Van Nieuwerburgh, Dieter Deforce, et al. 2024. “MicroRNA-146b Negatively Affects Bovine Embryo Development and Quality.” REPRODUCTION 167 (2). https://doi.org/10.1530/REP-23-0155.
Chicago author-date (all authors)
Pavani, Krishna Chaitanya, Guan XueFeng, Jayendra Chunduru, Tim Meese, Luc Peelman, Filip Van Nieuwerburgh, Dieter Deforce, An Hendrix, Kelly Tilleman, Ann Van Soom, and Katrien Smits. 2024. “MicroRNA-146b Negatively Affects Bovine Embryo Development and Quality.” REPRODUCTION 167 (2). doi:10.1530/REP-23-0155.
Vancouver
1.
Pavani KC, XueFeng G, Chunduru J, Meese T, Peelman L, Van Nieuwerburgh F, et al. MicroRNA-146b negatively affects bovine embryo development and quality. REPRODUCTION. 2024;167(2).
IEEE
[1]
K. C. Pavani et al., “MicroRNA-146b negatively affects bovine embryo development and quality,” REPRODUCTION, vol. 167, no. 2, 2024.
@article{01HV0Y9F92Q3JA0DG81NM82BBP,
  abstract     = {{MicroRNAs (miRNAs), which can be carried inside extracellular vesicles (EVs), play a crucial role in regulating embryo development up to the blastocyst stage. Yet, the molecular mechanisms underlying blastocyst development and quality are largely unknown. Recently, our group identified 69 differentially expressed miRNAs in extracellular vesicles (EVs) isolated from culture medium conditioned by bovine embryos that either developed to the blastocyst stage or did not (non-blastocysts). We found miR-146b to be more abundant in the EVs derived from media conditioned by non-blastocyst embryos. Using RT-qPCR, we here confirmed the upregulation of miR-146b in non-blastocyst (arrested at two- to four-cell and morula stage) embryos compared to blastocysts (P < 0.005), which coincides with the upregulation of miR-146b in EVs derived from the medium of these non-blastocysts. To evaluate a functional effect, bovine embryo culture media were supplemented with miR-146b mimics, resulting in significantly decreased embryo quality, with lower blastocyst rates at day 7 and lower total cell numbers, while the opposite was found after supplementation with miR-146b inhibitors, which resulted in reduced apoptosis rates (P < 0.01). Transcriptomic analysis of embryos treated with miR-146b mimics or inhibitors showed differential expression (P < 0.01) of genes associated with apoptosis, cell differentiation, and the RNA Pol II transcription complex, including WDR36, MBNL2, ERCC6l2, PYGO1, and SNIP1. Overall, miR-146b is overexpressed in non-blastocyst embryos and in EVs secreted by these embryos, and it regulates genes involved in embryo development and apoptosis, resulting in decreased embryo quality.}},
  articleno    = {{e230155}},
  author       = {{Pavani, Krishna Chaitanya and XueFeng, Guan and  Chunduru, Jayendra and Meese, Tim and Peelman, Luc and Van Nieuwerburgh, Filip and Deforce, Dieter and Hendrix, An and Tilleman, Kelly and Van Soom, Ann and Smits, Katrien}},
  issn         = {{1470-1626}},
  journal      = {{REPRODUCTION}},
  keywords     = {{CULTURE-MEDIA,PREIMPLANTATION EMBRYOS,EXTRACELLULAR VESICLES,EXPRESSION,COMMUNICATION,CANCER,RNA}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{11}},
  title        = {{MicroRNA-146b negatively affects bovine embryo development and quality}},
  url          = {{http://doi.org/10.1530/REP-23-0155}},
  volume       = {{167}},
  year         = {{2024}},
}

Altmetric
View in Altmetric
Web of Science
Times cited: