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Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export

Wei Ying, Yaowei Wang, Hong Wei, Yongming Luo (UGent) , Qian Ma (UGent) , Heyuan Zhu (UGent) , Hilde Janssens (UGent) , Nemanja Vukasinovic (UGent) , Miroslav Kvasnica, Johan Winne (UGent) , et al.
(2024) SCIENCE. 383(6689).
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Abstract
Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.
Keywords
Multidisciplinary, AUXIN TRANSPORT, P-GLYCOPROTEIN, CRYO-EM, TWISTED DWARF1, KINASE, INTERACTS, EFFLUX, ROOT, EXPRESSION, BINDING

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MLA
Ying, Wei, et al. “Structure and Function of the Arabidopsis ABC Transporter ABCB19 in Brassinosteroid Export.” SCIENCE, vol. 383, no. 6689, 2024, doi:10.1126/science.adj4591.
APA
Ying, W., Wang, Y., Wei, H., Luo, Y., Ma, Q., Zhu, H., … Sun, L. (2024). Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. SCIENCE, 383(6689). https://doi.org/10.1126/science.adj4591
Chicago author-date
Ying, Wei, Yaowei Wang, Hong Wei, Yongming Luo, Qian Ma, Heyuan Zhu, Hilde Janssens, et al. 2024. “Structure and Function of the Arabidopsis ABC Transporter ABCB19 in Brassinosteroid Export.” SCIENCE 383 (6689). https://doi.org/10.1126/science.adj4591.
Chicago author-date (all authors)
Ying, Wei, Yaowei Wang, Hong Wei, Yongming Luo, Qian Ma, Heyuan Zhu, Hilde Janssens, Nemanja Vukasinovic, Miroslav Kvasnica, Johan Winne, Yongxiang Gao, Shutang Tan, Jiří Friml, Xin Liu, Eugenia Russinova, and Linfeng Sun. 2024. “Structure and Function of the Arabidopsis ABC Transporter ABCB19 in Brassinosteroid Export.” SCIENCE 383 (6689). doi:10.1126/science.adj4591.
Vancouver
1.
Ying W, Wang Y, Wei H, Luo Y, Ma Q, Zhu H, et al. Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export. SCIENCE. 2024;383(6689).
IEEE
[1]
W. Ying et al., “Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export,” SCIENCE, vol. 383, no. 6689, 2024.
@article{01HTMBK3P3PPNB73YFCMFXP3ZZ,
  abstract     = {{Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In Arabidopsis thaliana, ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.}},
  articleno    = {{eadj4591}},
  author       = {{Ying, Wei and Wang, Yaowei and Wei, Hong and Luo, Yongming and Ma, Qian and Zhu, Heyuan and Janssens, Hilde and Vukasinovic, Nemanja and Kvasnica, Miroslav and Winne, Johan and Gao, Yongxiang and Tan, Shutang and Friml, Jiří and Liu, Xin and Russinova, Eugenia and Sun, Linfeng}},
  issn         = {{0036-8075}},
  journal      = {{SCIENCE}},
  keywords     = {{Multidisciplinary,AUXIN TRANSPORT,P-GLYCOPROTEIN,CRYO-EM,TWISTED DWARF1,KINASE,INTERACTS,EFFLUX,ROOT,EXPRESSION,BINDING}},
  language     = {{eng}},
  number       = {{6689}},
  pages        = {{12}},
  title        = {{Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export}},
  url          = {{http://doi.org/10.1126/science.adj4591}},
  volume       = {{383}},
  year         = {{2024}},
}
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