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Hormone profiling of chrysanthemum hairy roots after co-cultivation with Rhizobium rhizogenes

ellen de keyser (UGent) , J. Tyburski, J. Kesy, N. Mucha, T. Eeckhaut and Emmy Dhooghe (UGent)
(2023) XXVII international EUCARPIA symposium section ornamentals : from nature to culture - breeding ornamentals for sustainability. In Acta Horticulturae 1383. p.209-215
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Abstract
We have used chrysanthemum as a model crop to determine auxin and cytokinin levels in hairy roots formed after coculture of leaf discs with Rhizobium rhizogenes. All 6 genotypes tested formed hairy roots, upon which the presence of T-DNA genes was tested with a qPCR assay, evaluating the presence of TL- as well as TR-genes. Transformation efficiencies varied between 14 and 100%, and for every cultivar, roots with either only TL-genes and roots with both TL- and TR-genes were found. 2-isopentenyladening (2iP) and indole-3-acetic acid (IAA) levels were compared for all 12 roots that yielded sufficient material for analysis. In two cultivars, multiple TL+TR positive independent root lines could be compared, showing a significant difference for as well auxin as cytokinin levels. However, both for 2iP and IAA, 10-fold differences were detected between roots induced on leaves of a single cultivar. This suggests that phytohormone levels, and probably subsequent hairy root regeneration, depend on individual pRi-gene integration for each separate hairy root.
Keywords
Horticulture, auxins, chrysanthemum, cytokinins, LC-MS/MS, regeneration, Rhizobium, rhizogenes

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MLA
de keyser, ellen, et al. “Hormone Profiling of Chrysanthemum Hairy Roots after Co-Cultivation with Rhizobium Rhizogenes.” XXVII International EUCARPIA Symposium Section Ornamentals : From Nature to Culture - Breeding Ornamentals for Sustainability, edited by M. Mariotti and M. Beruto, vol. 1383, International Society for Horticultural Science (ISHS), 2023, pp. 209–15, doi:10.17660/actahortic.2023.1383.25.
APA
de keyser, ellen, Tyburski, J., Kesy, J., Mucha, N., Eeckhaut, T., & Dhooghe, E. (2023). Hormone profiling of chrysanthemum hairy roots after co-cultivation with Rhizobium rhizogenes. In M. Mariotti & M. Beruto (Eds.), XXVII international EUCARPIA symposium section ornamentals : from nature to culture - breeding ornamentals for sustainability (Vol. 1383, pp. 209–215). https://doi.org/10.17660/actahortic.2023.1383.25
Chicago author-date
keyser, ellen de, J. Tyburski, J. Kesy, N. Mucha, T. Eeckhaut, and Emmy Dhooghe. 2023. “Hormone Profiling of Chrysanthemum Hairy Roots after Co-Cultivation with Rhizobium Rhizogenes.” In XXVII International EUCARPIA Symposium Section Ornamentals : From Nature to Culture - Breeding Ornamentals for Sustainability, edited by M. Mariotti and M. Beruto, 1383:209–15. International Society for Horticultural Science (ISHS). https://doi.org/10.17660/actahortic.2023.1383.25.
Chicago author-date (all authors)
de keyser, ellen, J. Tyburski, J. Kesy, N. Mucha, T. Eeckhaut, and Emmy Dhooghe. 2023. “Hormone Profiling of Chrysanthemum Hairy Roots after Co-Cultivation with Rhizobium Rhizogenes.” In XXVII International EUCARPIA Symposium Section Ornamentals : From Nature to Culture - Breeding Ornamentals for Sustainability, ed by. M. Mariotti and M. Beruto, 1383:209–215. International Society for Horticultural Science (ISHS). doi:10.17660/actahortic.2023.1383.25.
Vancouver
1.
de keyser ellen, Tyburski J, Kesy J, Mucha N, Eeckhaut T, Dhooghe E. Hormone profiling of chrysanthemum hairy roots after co-cultivation with Rhizobium rhizogenes. In: Mariotti M, Beruto M, editors. XXVII international EUCARPIA symposium section ornamentals : from nature to culture - breeding ornamentals for sustainability. International Society for Horticultural Science (ISHS); 2023. p. 209–15.
IEEE
[1]
ellen de keyser, J. Tyburski, J. Kesy, N. Mucha, T. Eeckhaut, and E. Dhooghe, “Hormone profiling of chrysanthemum hairy roots after co-cultivation with Rhizobium rhizogenes,” in XXVII international EUCARPIA symposium section ornamentals : from nature to culture - breeding ornamentals for sustainability, Genoa, Italy, 2023, vol. 1383, pp. 209–215.
@inproceedings{01HQNDC186FZM39P6AC0CMAW6N,
  abstract     = {{We have used chrysanthemum as a model crop to determine auxin and cytokinin levels in hairy roots formed after coculture of leaf discs with Rhizobium rhizogenes. All 6 genotypes tested formed hairy roots, upon which the presence of T-DNA genes was tested with a qPCR assay, evaluating the presence of TL- as well as TR-genes. Transformation efficiencies varied between 14 and 100%, and for every cultivar, roots with either only TL-genes and roots with both TL- and TR-genes were found. 2-isopentenyladening (2iP) and indole-3-acetic acid (IAA) levels were compared for all 12 roots that yielded sufficient material for analysis. In two cultivars, multiple TL+TR positive independent root lines could be compared, showing a significant difference for as well auxin as cytokinin levels. However, both for 2iP and IAA, 10-fold differences were detected between roots induced on leaves of a single cultivar. This suggests that phytohormone levels, and probably subsequent hairy root regeneration, depend on individual pRi-gene integration for each separate hairy root.}},
  author       = {{de keyser, ellen and Tyburski, J. and Kesy, J. and Mucha, N. and Eeckhaut, T. and Dhooghe, Emmy}},
  booktitle    = {{XXVII international EUCARPIA symposium section ornamentals : from nature to culture - breeding ornamentals for sustainability}},
  editor       = {{Mariotti, M. and Beruto, M.}},
  isbn         = {{9789462613829}},
  issn         = {{0567-7572}},
  keywords     = {{Horticulture,auxins,chrysanthemum,cytokinins,LC-MS/MS,regeneration,Rhizobium,rhizogenes}},
  language     = {{eng}},
  location     = {{Genoa, Italy}},
  pages        = {{209--215}},
  publisher    = {{International Society for Horticultural Science (ISHS)}},
  title        = {{Hormone profiling of chrysanthemum hairy roots after co-cultivation with Rhizobium rhizogenes}},
  url          = {{http://doi.org/10.17660/actahortic.2023.1383.25}},
  volume       = {{1383}},
  year         = {{2023}},
}
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