@article{01HQATMN56J07806C6RG8BK0GM,
  abstract     = {{Pluripotent stem cell (PSC)-derived cardiomyocytes offer vast potential in heart failure therapy, yet their immaturity poses challenges. To solve this issue, a multifaceted approach combining exclusive biochemical/topographical cues in the design of substrates mimicking the cardiac extracellular matrix was followed. Firstly, polycaprolactone (PCL)/chitosan nanofibers were electrospun in a random and aligned fashion, thus forming myocardium-like constructs. The scaffolds were then subjected to Ar and N2 dielectric barrier discharge treatments, thus further improving their surface properties. The Ar plasma incorporated oxygen-containing functionalities onto the nanofibers surface with an additional implantation of nitrogen-containing groups upon N2 plasma treatment, which both enhanced fibers’ wettability. No topographical/dimensional damages were detected post-plasma treatments, except for a slight decrease in the surface roughness of the aligned nanofibers, which led to a decrease in their tensile stress. Both plasmas significantly enhanced the adhesion of PSC-derived cardiomyocytes that displayed more circular versus highly elongated body/nucleus morphologies on random versus aligned nanofibers, respectively. Interestingly, the cell alignment was less pronounced on GeltrexTM-coated nanofibers. Furthermore, increased sarcomere distance and organization were observed on aligned plasma-treated nanofibers, suggesting an enhanced cell maturation. Overall, PCL/chitosan nanofibers with aligned orientation and plasma-induced surface chemistry hold promise in cardiac tissue engineering applications.}},
  articleno    = {{159680}},
  author       = {{Léger, Laurens and Aliakbarshirazi, Sheida and Zahedifar, Pegah and Aalders, Jeffrey and Van Der Voort, Pascal and De Geyter, Nathalie and Morent, Rino and van Hengel, Jolanda and Ghobeira, Rouba}},
  issn         = {{0169-4332}},
  journal      = {{APPLIED SURFACE SCIENCE}},
  keywords     = {{Electrospinning,PCL/chitosan,Aligned nanofibers,Plasma treatment,Surface analysis,Stem cell-derived cardiomyocytes,Cardiac tissue engineering,FIBROUS SCAFFOLDS,MATURATION,DIFFERENTIATION,ORGANIZATION,ENHANCE,ALIGNMENT,MYOCYTES,MATRICES,MUSCLE,MESHES}},
  language     = {{eng}},
  pages        = {{20}},
  title        = {{Combinatorial effects of surface plasma-treating and aligning PCL/chitosan nanofibers on the behavior of stem cell-derived cardiomyocytes for cardiac tissue engineering}},
  url          = {{http://doi.org/10.1016/j.apsusc.2024.159680}},
  volume       = {{655}},
  year         = {{2024}},
}

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