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Abstract
. GBS is an efficient genotyping tool for heterozygous and polyploid species, yet the fraction of missing data, inherent to the method, may be detrimental to genetic analyses. We tested several restriction enzymes to choose the ones that were likely to provide the maximum number of loci with little missing data considering a certain sequencing effort. For lucerne and cocksfoot, the enzymes that met the target of about 10 000 loci were the combination of PstI-MseI for lucerne and PstI for cocksfoot. 1 066 lucerne accessions were genotyped with PstI-MseI, and more than 200 000 SNP with less than 5% missing data were obtained. Based on these results, we recommend performing such a test to optimize GBS genotyping efforts in forage species.
Keywords
cocksfoot, heterozygous, lucerne, polyploidy, SNP

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MLA
Julier, Bernadette, et al. “Optimisation of GBS Protocols for Efficient Genotyping of Forage Species.” Exploiting Genetic Diversity of Forages to Fulfil Their Economic and Environmental Roles, Univerzita Palackého v Olomouci, 2021, pp. 71–74, doi:10.5507/vup.21.24459677.17.
APA
Julier, B., Blugeon, S., Delaunay, S., Mappa, G., Ruttink, T., Pégard, M., & Barre, P. (2021). Optimisation of GBS protocols for efficient genotyping of forage species. Exploiting Genetic Diversity of Forages to Fulfil Their Economic and Environmental Roles, 71–74. https://doi.org/10.5507/vup.21.24459677.17
Chicago author-date
Julier, Bernadette, Sébastien Blugeon, Sabrina Delaunay, Gaëtan Mappa, Tom Ruttink, Marie Pégard, and Philippe Barre. 2021. “Optimisation of GBS Protocols for Efficient Genotyping of Forage Species.” In Exploiting Genetic Diversity of Forages to Fulfil Their Economic and Environmental Roles, 71–74. Křížkovského 8, 771 47 Olomouc: Univerzita Palackého v Olomouci. https://doi.org/10.5507/vup.21.24459677.17.
Chicago author-date (all authors)
Julier, Bernadette, Sébastien Blugeon, Sabrina Delaunay, Gaëtan Mappa, Tom Ruttink, Marie Pégard, and Philippe Barre. 2021. “Optimisation of GBS Protocols for Efficient Genotyping of Forage Species.” In Exploiting Genetic Diversity of Forages to Fulfil Their Economic and Environmental Roles, 71–74. Křížkovského 8, 771 47 Olomouc: Univerzita Palackého v Olomouci. doi:10.5507/vup.21.24459677.17.
Vancouver
1.
Julier B, Blugeon S, Delaunay S, Mappa G, Ruttink T, Pégard M, et al. Optimisation of GBS protocols for efficient genotyping of forage species. In: Exploiting genetic diversity of forages to fulfil their economic and environmental roles. Křížkovského 8, 771 47 Olomouc: Univerzita Palackého v Olomouci; 2021. p. 71–4.
IEEE
[1]
B. Julier et al., “Optimisation of GBS protocols for efficient genotyping of forage species,” in Exploiting genetic diversity of forages to fulfil their economic and environmental roles, online, 2021, pp. 71–74.
@inproceedings{01HQ12JDWJD31CPC77MNZ4T798,
  abstract     = {{. GBS is an efficient genotyping tool for heterozygous and polyploid species, yet the fraction of missing data, inherent to the method, may be detrimental to genetic analyses. We tested several restriction enzymes to choose the ones that were likely to provide the maximum number of loci with little missing data considering a certain sequencing effort. For lucerne and cocksfoot, the enzymes that met the target of about 10 000 loci were the combination of PstI-MseI for lucerne and PstI for cocksfoot. 1 066 lucerne accessions were genotyped with PstI-MseI, and more than 200 000 SNP with less than 5% missing data were obtained. Based on these results, we recommend performing such a test to optimize GBS genotyping efforts in forage species.}},
  author       = {{Julier, Bernadette and Blugeon, Sébastien and Delaunay, Sabrina and Mappa, Gaëtan and Ruttink, Tom and Pégard, Marie and Barre, Philippe}},
  booktitle    = {{Exploiting genetic diversity of forages to fulfil their economic and environmental roles}},
  keywords     = {{cocksfoot, heterozygous, lucerne, polyploidy, SNP}},
  language     = {{und}},
  location     = {{online}},
  pages        = {{71--74}},
  publisher    = {{Univerzita Palackého v Olomouci}},
  title        = {{Optimisation of GBS protocols for efficient genotyping of forage species}},
  url          = {{http://doi.org/10.5507/vup.21.24459677.17}},
  year         = {{2021}},
}

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