@article{01HPH734144AQ5H51S16VYSMSK,
  abstract     = {{Up to 50 % of dairy cows fail to resolve uterine involution and develop chronic clinical (CE) or subclinical endometritis (SE) 21 days after calving. Clinical endometritis is associated with purulent discharge, while SE is not associated with overt clinical signs. Along with numerous knowledge gaps related to its pathogenesis, SE does not allow for a straightforward and effective therapy. Therefore, it is crucial to unravel differences in the expression of genes among healthy, CE, and SE cows. This might contribute to the discovery of new drug candidates and, in consequence, a potentially effective treatment. In the present study, cows between 21 and 28 days postpartum (PP) were examined using vaginoscopy for the presence of vaginal discharge and endometrial cytology for the determination of the endometrial polymorphonuclear cell (PMN) percentage. Next, an endometrial biopsy sample was taken to investigate the expression of 13 selected candidate genes by qPCR. Uterine health status was assigned to healthy (absence of abnormal vaginal discharge and <= 5 % PMN, n = 13), SE (absence of abnormal vaginal discharge and >5 % PMN, n = 30), and CE (mucopurulent or purulent vaginal discharge and >5 % PMN, n = 9). At the same time, a blood sample was collected to assess serum progesterone concentration and to categorize cows as low (<= 1 ng/mL) or high (>1 ng/mL) in progesterone. High expression of IL1B, IL6, IL17A, CXCL8, PTGES, PTGS1, PTGS2, and INHBA genes and low expression of FST was noted in the endometrium of CE compared to healthy cows. Increased endometrial INHBA expression was observed in both SE and CE compared to healthy cows. Interestingly, greater expression of PTGES and PRXL2B genes and lower expression of PTGS2 were characteristic of SE versus CE or healthy. Among cows with no overt clinical symptoms of uterine disease (healthy and SE), the endometrial expression of IL1 B, CXCL8, and PTGES was greater in cows with high versus low serum progesterone. Several genes were differentially expressed among healthy, SE, and CE cows indicating different pathways for the development of different uterine diseases. In conclusion, we found progesterone-independent SE markers, which suggests that low endometrial PTGS2 expression may be indicative of an inadequate immune response and thus contribute to the pathogenesis of SE.}},
  author       = {{Tobolski, Dawid and  Zwierzchowski, Grzegorz and  Lukasik, Karolina and  Skarzynskic, Dariusz Jan and Bogado Pascottini, Osvaldo Américo and Opsomer, Geert and  Baranski, Wojciech}},
  issn         = {{0093-691X}},
  journal      = {{THERIOGENOLOGY}},
  keywords     = {{Uterine disease,Inflammation,Gene expression,Progesterone,POSTPARTUM UTERINE DISEASE,ESTROUS-CYCLE,ACTIVIN-A,BOVINE ENDOMETRIUM,REPRODUCTIVE-PERFORMANCE,INFLAMMATORY RESPONSE,GENE-EXPRESSION,BINDING-PROTEIN,FOLLISTATIN,DIAGNOSIS}},
  language     = {{eng}},
  pages        = {{146--154}},
  publisher    = {{Elsevier Science Inc}},
  title        = {{Progesterone-independent endometrial mRNA expression in dairy cows with clinical or subclinical endometritis}},
  url          = {{http://doi.org/10.1016/j.theriogenology.2023.12.031}},
  volume       = {{216}},
  year         = {{2024}},
}

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