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Characterization of the semen microbiota of healthy stud dogs using 16S RNA sequencing

Penelope Banchi (UGent) , L. Bertolotti, Lotte Spanoghe (UGent) , Hiba Ali Hassan, Joke Lannoo (UGent) , Guillaume Domain (UGent) , K. S. Henzel, V. Gaillard, A. Rota and Ann Van Soom (UGent)
(2024) THERIOGENOLOGY. 216. p.1-7
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Abstract
The reproductive microbiota of male dogs has never been investigated using culture-independent sequencing techniques. The purpose of the present study was to get seminal knowledge on the microbiota of the ejaculate. Specifically, factors as the fraction of the ejaculate, the sperm quality (normospermia, teratozoospermia), and the living environment were evaluated. The sperm-rich and the prostatic fractions of the ejaculate were collected from healthy stud dogs. Following the sperm analysis, samples from twenty animals (normospermic n = 10 and teratozoospermic n = 10) were stored at - 80 C-degrees until further processing including DNA extraction and 16S rRNA sequencing. Alpha- (Shannon index) and beta- (Bray-Curtis, Unweighted UniFrac) diversities were assessed and compared (PERMANOVA) based on the group of samples (biological samples from the ejaculate and controls), the fraction of the ejaculate (sperm-rich and prostatic fractions), the animal group (normospermia and teratozoospermia), and the living environment of the animal (kennel or pet living in-house). The most abundant bacterial phyla in canine semen samples were Proteobacteria, Firmicutes, and Actinobacteria. Overall, the dominant bacterial family was that of Pasteurellaceae The genus Mycoplasma was never detected. No differences in terms of bacterial composition were found based on the fraction of the ejaculate and based on the animal group (P > 0.05). On the other hand, differences in alpha and beta diversities were highlighted based on the living environment (P = 0.001).Overall, the results of the present study provide preliminary insights on dog semen microbiota, opening a new chapter in the field of canine andrology. Our results suggest that the environment may play a role in influencing the reproductive microbiota of male dogs and that the prostatic fraction of the ejaculate can be used for further research as a representative of the semen microbiota.
Keywords
INFECTION, QUALITY, GENE, Sperm, Reproductive microbiota, Canine, Male, 16S rRNA sequencing

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MLA
Banchi, Penelope, et al. “Characterization of the Semen Microbiota of Healthy Stud Dogs Using 16S RNA Sequencing.” THERIOGENOLOGY, vol. 216, Elsevier Science Inc, 2024, pp. 1–7, doi:10.1016/j.theriogenology.2023.12.018.
APA
Banchi, P., Bertolotti, L., Spanoghe, L., Ali Hassan, H., Lannoo, J., Domain, G., … Van Soom, A. (2024). Characterization of the semen microbiota of healthy stud dogs using 16S RNA sequencing. THERIOGENOLOGY, 216, 1–7. https://doi.org/10.1016/j.theriogenology.2023.12.018
Chicago author-date
Banchi, Penelope, L. Bertolotti, Lotte Spanoghe, Hiba Ali Hassan, Joke Lannoo, Guillaume Domain, K. S. Henzel, V. Gaillard, A. Rota, and Ann Van Soom. 2024. “Characterization of the Semen Microbiota of Healthy Stud Dogs Using 16S RNA Sequencing.” THERIOGENOLOGY 216: 1–7. https://doi.org/10.1016/j.theriogenology.2023.12.018.
Chicago author-date (all authors)
Banchi, Penelope, L. Bertolotti, Lotte Spanoghe, Hiba Ali Hassan, Joke Lannoo, Guillaume Domain, K. S. Henzel, V. Gaillard, A. Rota, and Ann Van Soom. 2024. “Characterization of the Semen Microbiota of Healthy Stud Dogs Using 16S RNA Sequencing.” THERIOGENOLOGY 216: 1–7. doi:10.1016/j.theriogenology.2023.12.018.
Vancouver
1.
Banchi P, Bertolotti L, Spanoghe L, Ali Hassan H, Lannoo J, Domain G, et al. Characterization of the semen microbiota of healthy stud dogs using 16S RNA sequencing. THERIOGENOLOGY. 2024;216:1–7.
IEEE
[1]
P. Banchi et al., “Characterization of the semen microbiota of healthy stud dogs using 16S RNA sequencing,” THERIOGENOLOGY, vol. 216, pp. 1–7, 2024.
@article{01HPH6AVTWFM4BK8WGKPJXR8AA,
  abstract     = {{The reproductive microbiota of male dogs has never been investigated using culture-independent sequencing techniques. The purpose of the present study was to get seminal knowledge on the microbiota of the ejaculate. Specifically, factors as the fraction of the ejaculate, the sperm quality (normospermia, teratozoospermia), and the living environment were evaluated. The sperm-rich and the prostatic fractions of the ejaculate were collected from healthy stud dogs. Following the sperm analysis, samples from twenty animals (normospermic n = 10 and teratozoospermic n = 10) were stored at - 80 C-degrees until further processing including DNA extraction and 16S rRNA sequencing. Alpha- (Shannon index) and beta- (Bray-Curtis, Unweighted UniFrac) diversities were assessed and compared (PERMANOVA) based on the group of samples (biological samples from the ejaculate and controls), the fraction of the ejaculate (sperm-rich and prostatic fractions), the animal group (normospermia and teratozoospermia), and the living environment of the animal (kennel or pet living in-house). The most abundant bacterial phyla in canine semen samples were Proteobacteria, Firmicutes, and Actinobacteria. Overall, the dominant bacterial family was that of Pasteurellaceae The genus Mycoplasma was never detected. No differences in terms of bacterial composition were found based on the fraction of the ejaculate and based on the animal group (P > 0.05). On the other hand, differences in alpha and beta diversities were highlighted based on the living environment (P = 0.001).Overall, the results of the present study provide preliminary insights on dog semen microbiota, opening a new chapter in the field of canine andrology. Our results suggest that the environment may play a role in influencing the reproductive microbiota of male dogs and that the prostatic fraction of the ejaculate can be used for further research as a representative of the semen microbiota.}},
  author       = {{Banchi, Penelope and  Bertolotti, L. and Spanoghe, Lotte and Ali Hassan, Hiba and Lannoo, Joke and Domain, Guillaume and  Henzel, K. S. and  Gaillard, V. and  Rota, A. and Van Soom, Ann}},
  issn         = {{0093-691X}},
  journal      = {{THERIOGENOLOGY}},
  keywords     = {{INFECTION,QUALITY,GENE,Sperm,Reproductive microbiota,Canine,Male,16S rRNA sequencing}},
  language     = {{eng}},
  pages        = {{1--7}},
  publisher    = {{Elsevier Science Inc}},
  title        = {{Characterization of the semen microbiota of healthy stud dogs using 16S RNA sequencing}},
  url          = {{http://doi.org/10.1016/j.theriogenology.2023.12.018}},
  volume       = {{216}},
  year         = {{2024}},
}
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