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Serum symmetric dimethylarginine in older dogs : reference interval and comparison of a gold standard method with the ELISA

Sofie Marynissen (UGent) , Greet Junius, Evi van den Steen, Lisbeth Patteet, Luc Duchateau (UGent) , Siska Croubels (UGent) , Sylvie Daminet (UGent) and Dominique Paepe (UGent)
(2024) JOURNAL OF VETERINARY INTERNAL MEDICINE. 38(2). p.960-970
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Abstract
Background: Serum symmetric dimethylarginine (SDMA) is used to screen for renal dysfunction in dogs. The gold standard technique for measuring SDMA, liquid chromatography-tandem mass spectrometry (LC-MS/MS) is not widely available. Age-specific reference intervals for SDMA in older dogs are lacking.Objectives: Prospective study in older dogs to validate a commercially available LC-MS/MS method for SDMA, compare SDMA concentrations with concentrations measured using ELISA and obtain a reference interval (RI) for older dogs using both methods.Animals: Client-owned older dogs undergoing health screening.Methods: The LC-MS/MS method was analytically validated (limit of detection, precision, and linearity). Serum was sent cooled overnight for ELISA or was frozen at -80 degrees C until batch analysis using LC-MS/MS. Results of LC-MS/MS and ELISA were compared and RIs for older dogs were calculated according to international guidelines.Results: The LC-MS/MS method showed good linearity (r(2) = .99) and precision (coefficient of variation <10%), with a laboratory RI between 8.0 and 14.0 mu g/dL. Paired measurements were available from 118 different dogs. Median SDMA concentration were 9.4 (range, 5.0-21.2) using LC-MS/MS and 12.0 (range, 5.0-22.0) mu g/dL using ELISA. Both methods significantly differed with a mean difference of 2.2 mu g/dL. The RI for older dogs for LC-MS/MS was 4.4-15.0 mu g/dL, and for ELISA was 6.4-17.4 mu g/dL.Conclusions and Clinical Importance: The ELISA provided significantly higher SDMA concentrations compared to the validated LC-MS/MS method, indicating the need for device- or assay-specific RI. The obtained age-specific RI for SDMA is considerably higher in older dogs compared to the general laboratory RI.
Keywords
asymmetric dimethylarginine, canine, ELISA, SDMA, EXOGENOUS CREATININE CLEARANCE, CHRONIC KIDNEY-DISEASE, ASYMMETRIC DIMETHYLARGININE, RENAL-FUNCTION, MARKER, AGE, BIOMARKERS, VALUES, MASS

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MLA
Marynissen, Sofie, et al. “Serum Symmetric Dimethylarginine in Older Dogs : Reference Interval and Comparison of a Gold Standard Method with the ELISA.” JOURNAL OF VETERINARY INTERNAL MEDICINE, vol. 38, no. 2, Wiley, 2024, pp. 960–70, doi:10.1111/jvim.16981.
APA
Marynissen, S., Junius, G., van den Steen, E., Patteet, L., Duchateau, L., Croubels, S., … Paepe, D. (2024). Serum symmetric dimethylarginine in older dogs : reference interval and comparison of a gold standard method with the ELISA. JOURNAL OF VETERINARY INTERNAL MEDICINE, 38(2), 960–970. https://doi.org/10.1111/jvim.16981
Chicago author-date
Marynissen, Sofie, Greet Junius, Evi van den Steen, Lisbeth Patteet, Luc Duchateau, Siska Croubels, Sylvie Daminet, and Dominique Paepe. 2024. “Serum Symmetric Dimethylarginine in Older Dogs : Reference Interval and Comparison of a Gold Standard Method with the ELISA.” JOURNAL OF VETERINARY INTERNAL MEDICINE 38 (2): 960–70. https://doi.org/10.1111/jvim.16981.
Chicago author-date (all authors)
Marynissen, Sofie, Greet Junius, Evi van den Steen, Lisbeth Patteet, Luc Duchateau, Siska Croubels, Sylvie Daminet, and Dominique Paepe. 2024. “Serum Symmetric Dimethylarginine in Older Dogs : Reference Interval and Comparison of a Gold Standard Method with the ELISA.” JOURNAL OF VETERINARY INTERNAL MEDICINE 38 (2): 960–970. doi:10.1111/jvim.16981.
Vancouver
1.
Marynissen S, Junius G, van den Steen E, Patteet L, Duchateau L, Croubels S, et al. Serum symmetric dimethylarginine in older dogs : reference interval and comparison of a gold standard method with the ELISA. JOURNAL OF VETERINARY INTERNAL MEDICINE. 2024;38(2):960–70.
IEEE
[1]
S. Marynissen et al., “Serum symmetric dimethylarginine in older dogs : reference interval and comparison of a gold standard method with the ELISA,” JOURNAL OF VETERINARY INTERNAL MEDICINE, vol. 38, no. 2, pp. 960–970, 2024.
@article{01HP3YQF607K9ASTNQ21YAA82S,
  abstract     = {{Background: Serum symmetric dimethylarginine (SDMA) is used to screen for renal dysfunction in dogs. The gold standard technique for measuring SDMA, liquid chromatography-tandem mass spectrometry (LC-MS/MS) is not widely available. Age-specific reference intervals for SDMA in older dogs are lacking.Objectives: Prospective study in older dogs to validate a commercially available LC-MS/MS method for SDMA, compare SDMA concentrations with concentrations measured using ELISA and obtain a reference interval (RI) for older dogs using both methods.Animals: Client-owned older dogs undergoing health screening.Methods: The LC-MS/MS method was analytically validated (limit of detection, precision, and linearity). Serum was sent cooled overnight for ELISA or was frozen at -80 degrees C until batch analysis using LC-MS/MS. Results of LC-MS/MS and ELISA were compared and RIs for older dogs were calculated according to international guidelines.Results: The LC-MS/MS method showed good linearity (r(2) = .99) and precision (coefficient of variation <10%), with a laboratory RI between 8.0 and 14.0 mu g/dL. Paired measurements were available from 118 different dogs. Median SDMA concentration were 9.4 (range, 5.0-21.2) using LC-MS/MS and 12.0 (range, 5.0-22.0) mu g/dL using ELISA. Both methods significantly differed with a mean difference of 2.2 mu g/dL. The RI for older dogs for LC-MS/MS was 4.4-15.0 mu g/dL, and for ELISA was 6.4-17.4 mu g/dL.Conclusions and Clinical Importance: The ELISA provided significantly higher SDMA concentrations compared to the validated LC-MS/MS method, indicating the need for device- or assay-specific RI. The obtained age-specific RI for SDMA is considerably higher in older dogs compared to the general laboratory RI.}},
  author       = {{Marynissen, Sofie and  Junius, Greet and  van den Steen, Evi and Patteet, Lisbeth and Duchateau, Luc and Croubels, Siska and Daminet, Sylvie and Paepe, Dominique}},
  issn         = {{0891-6640}},
  journal      = {{JOURNAL OF VETERINARY INTERNAL MEDICINE}},
  keywords     = {{asymmetric dimethylarginine,canine,ELISA,SDMA,EXOGENOUS CREATININE CLEARANCE,CHRONIC KIDNEY-DISEASE,ASYMMETRIC DIMETHYLARGININE,RENAL-FUNCTION,MARKER,AGE,BIOMARKERS,VALUES,MASS}},
  language     = {{und}},
  number       = {{2}},
  pages        = {{960--970}},
  publisher    = {{Wiley}},
  title        = {{Serum symmetric dimethylarginine in older dogs : reference interval and comparison of a gold standard method with the ELISA}},
  url          = {{http://doi.org/10.1111/jvim.16981}},
  volume       = {{38}},
  year         = {{2024}},
}
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