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Oxidative stress in donor mares for ovum pick-up delays embryonic development

Mohamed Hedia (UGent) , Daniel Angel Velez (UGent) , Marion Papas (UGent) , Sofie Peere (UGent) , Ilse Gerits (UGent) , Tine De Coster (UGent) , Emma Van den Branden (UGent) , Jan Govaere (UGent) , Ann Van Soom (UGent) , Jo L. M. R. Leroy, et al.
(2024) THERIOGENOLOGY. 213. p.109-113
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Abstract
The in vitro production of equine embryos via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) has increased rapidly. There is a marked effect of the individual mare on the outcome of OPU-ICSI, but little is known about the influence of the mare's health condition. This study aimed to investigate the potential associations between the concentrations of interleukin-6 (IL-6), reactive oxygen metabolites (d-ROMs), and biological antioxidant potential (BAP) in serum of oocytes' donor mares and the subsequent embryonic development. Just before OPU, a blood sample was collected from 28 Warmblood donor mares, that were subjected to a routine OPU-ICSI program. The serum concentrations of IL-6, d-ROMs, and BAP were assayed photometrically. The maturation, cleavage and blastocyst rate as well as the kinetics of blastocyst development were recorded. The average blastocyst rate was 24.68 +/- 5.16% and the average concentrations of IL-6, d-ROMs, and BAP were 519.59 +/- 157.08 pg/mL, 171.30 +/- 4.55 carratelli units (UCARR), and 2711.30 +/- 4.55 mu mol/L, respectively. Serum concentrations of IL-6, d-ROMs, and BAP were not significantly different between mares yielding at least one blastocyst (552.68 +/- 235.18 pg/mL, 168.36 +/- 5.56 UCARR, and 2524.80 +/- 159.55 mu mol/L) and mares yielding no blastocysts (468.47 +/- 179.99 pg/mL, 175.85 +/- 7.89 UCARR, and 2999.50 +/- 300.13 mu mol/L, respectively). Serum concentrations of d-ROMs were significantly lower in mares with fast growing (at day 7-8 post ICSI; 148.10 +/- 8.13 UCARR) compared to those with slow growing blastocysts (>= day 9 post ICSI; 179.41 +/- 4.89 UCARR; P = 0.003). Taken together, the serum concentration of IL-6, d-ROMs, and BAP do not determine the mare's ability to produce blastocysts in vitro. Although it may be questioned whether a single sample is representative of the mare's health status, changes in serum metabolites related to oxidative stress at the time of oocyte retrieval were linked to a delayed blastocyst development in a clinical OPU-ICSI outcome.
Keywords
INTRACYTOPLASMIC SPERM INJECTION, FOLLICULAR-FLUID CYTOKINE, INTERLEUKIN-6, HORMONE, MATURATION, BIOMARKERS, OOCYTES, SERUM, AGE, IL-6, Oxidative stress, r-ROMs, BAP, OPU-ICSI, Mares

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MLA
Hedia, Mohamed, et al. “Oxidative Stress in Donor Mares for Ovum Pick-up Delays Embryonic Development.” THERIOGENOLOGY, vol. 213, Elsevier, 2024, pp. 109–13, doi:10.1016/j.theriogenology.2023.10.006.
APA
Hedia, M., Angel Velez, D., Papas, M., Peere, S., Gerits, I., De Coster, T., … Smits, K. (2024). Oxidative stress in donor mares for ovum pick-up delays embryonic development. THERIOGENOLOGY, 213, 109–113. https://doi.org/10.1016/j.theriogenology.2023.10.006
Chicago author-date
Hedia, Mohamed, Daniel Angel Velez, Marion Papas, Sofie Peere, Ilse Gerits, Tine De Coster, Emma Van den Branden, et al. 2024. “Oxidative Stress in Donor Mares for Ovum Pick-up Delays Embryonic Development.” THERIOGENOLOGY 213: 109–13. https://doi.org/10.1016/j.theriogenology.2023.10.006.
Chicago author-date (all authors)
Hedia, Mohamed, Daniel Angel Velez, Marion Papas, Sofie Peere, Ilse Gerits, Tine De Coster, Emma Van den Branden, Jan Govaere, Ann Van Soom, Jo L. M. R. Leroy, and Katrien Smits. 2024. “Oxidative Stress in Donor Mares for Ovum Pick-up Delays Embryonic Development.” THERIOGENOLOGY 213: 109–113. doi:10.1016/j.theriogenology.2023.10.006.
Vancouver
1.
Hedia M, Angel Velez D, Papas M, Peere S, Gerits I, De Coster T, et al. Oxidative stress in donor mares for ovum pick-up delays embryonic development. THERIOGENOLOGY. 2024;213:109–13.
IEEE
[1]
M. Hedia et al., “Oxidative stress in donor mares for ovum pick-up delays embryonic development,” THERIOGENOLOGY, vol. 213, pp. 109–113, 2024.
@article{01HNYR8WFAS7PNZXC2JQY1FV6B,
  abstract     = {{The in vitro production of equine embryos via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) has increased rapidly. There is a marked effect of the individual mare on the outcome of OPU-ICSI, but little is known about the influence of the mare's health condition. This study aimed to investigate the potential associations between the concentrations of interleukin-6 (IL-6), reactive oxygen metabolites (d-ROMs), and biological antioxidant potential (BAP) in serum of oocytes' donor mares and the subsequent embryonic development. Just before OPU, a blood sample was collected from 28 Warmblood donor mares, that were subjected to a routine OPU-ICSI program. The serum concentrations of IL-6, d-ROMs, and BAP were assayed photometrically. The maturation, cleavage and blastocyst rate as well as the kinetics of blastocyst development were recorded. The average blastocyst rate was 24.68 +/- 5.16% and the average concentrations of IL-6, d-ROMs, and BAP were 519.59 +/- 157.08 pg/mL, 171.30 +/- 4.55 carratelli units (UCARR), and 2711.30 +/- 4.55 mu mol/L, respectively. Serum concentrations of IL-6, d-ROMs, and BAP were not significantly different between mares yielding at least one blastocyst (552.68 +/- 235.18 pg/mL, 168.36 +/- 5.56 UCARR, and 2524.80 +/- 159.55 mu mol/L) and mares yielding no blastocysts (468.47 +/- 179.99 pg/mL, 175.85 +/- 7.89 UCARR, and 2999.50 +/- 300.13 mu mol/L, respectively). Serum concentrations of d-ROMs were significantly lower in mares with fast growing (at day 7-8 post ICSI; 148.10 +/- 8.13 UCARR) compared to those with slow growing blastocysts (>= day 9 post ICSI; 179.41 +/- 4.89 UCARR; P = 0.003). Taken together, the serum concentration of IL-6, d-ROMs, and BAP do not determine the mare's ability to produce blastocysts in vitro. Although it may be questioned whether a single sample is representative of the mare's health status, changes in serum metabolites related to oxidative stress at the time of oocyte retrieval were linked to a delayed blastocyst development in a clinical OPU-ICSI outcome.}},
  author       = {{Hedia, Mohamed and Angel Velez, Daniel and Papas, Marion and Peere, Sofie and Gerits, Ilse and De Coster, Tine and Van den Branden, Emma and Govaere, Jan and Van Soom, Ann and Leroy, Jo L. M. R. and Smits, Katrien}},
  issn         = {{0093-691X}},
  journal      = {{THERIOGENOLOGY}},
  keywords     = {{INTRACYTOPLASMIC SPERM INJECTION,FOLLICULAR-FLUID CYTOKINE,INTERLEUKIN-6,HORMONE,MATURATION,BIOMARKERS,OOCYTES,SERUM,AGE,IL-6,Oxidative stress,r-ROMs,BAP,OPU-ICSI,Mares}},
  language     = {{eng}},
  pages        = {{109--113}},
  publisher    = {{Elsevier}},
  title        = {{Oxidative stress in donor mares for ovum pick-up delays embryonic development}},
  url          = {{http://doi.org/10.1016/j.theriogenology.2023.10.006}},
  volume       = {{213}},
  year         = {{2024}},
}
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