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Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity

Tom Verbeelen, Celia Alvarez Fernandez (UGent) , Thanh Huy Nguyen, Surya Gupta, Raf Aarts, Kevin Tabury, Baptiste Leroy, Ruddy Wattiez, Siegfried E. Vlaeminck, Natalie Leys, et al.
(2024) NPJ MICROGRAVITY. 10(1).
Author
Organization
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Abstract
Regenerative life support systems (RLSS) will play a vital role in achieving self-sufficiency during long-distance space travel. Urine conversion into a liquid nitrate-based fertilizer is a key process in most RLSS. This study describes the effects of simulated microgravity (SMG) on Comamonas testosteroni, Nitrosomonas europaea, Nitrobacter winogradskyi and a tripartite culture of the three, in the context of nitrogen recovery for the Micro-Ecological Life Support System Alternative (MELiSSA). Rotary cell culture systems (RCCS) and random positioning machines (RPM) were used as SMG analogues. The transcriptional responses of the cultures were elucidated. For CO2-producing C. testosteroni and the tripartite culture, a PermaLifeTM PL-70 cell culture bag mounted on an in-house 3D-printed holder was applied to eliminate air bubble formation during SMG cultivation. Gene expression changes indicated that the fluid dynamics in SMG caused nutrient and O2 limitation. Genes involved in urea hydrolysis and nitrification were minimally affected, while denitrification-related gene expression was increased. The findings highlight potential challenges for nitrogen recovery in space.
Keywords
SHEAR MODELED MICROGRAVITY, BIOFILM LIFE-STYLE, NITROSOMONAS-EUROPAEA, NITROBACTER-WINOGRADSKYI, OXIDATIVE STRESS, GENE-EXPRESSION, COMAMONAS-TESTOSTERONI, OXIDIZING BACTERIUM, GENOME SEQUENCE, GROWTH

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MLA
Verbeelen, Tom, et al. “Whole Transcriptome Analysis Highlights Nutrient Limitation of Nitrogen Cycle Bacteria in Simulated Microgravity.” NPJ MICROGRAVITY, vol. 10, no. 1, 2024, doi:10.1038/s41526-024-00345-z.
APA
Verbeelen, T., Alvarez Fernandez, C., Nguyen, T. H., Gupta, S., Aarts, R., Tabury, K., … Mastroleo, F. (2024). Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity. NPJ MICROGRAVITY, 10(1). https://doi.org/10.1038/s41526-024-00345-z
Chicago author-date
Verbeelen, Tom, Celia Alvarez Fernandez, Thanh Huy Nguyen, Surya Gupta, Raf Aarts, Kevin Tabury, Baptiste Leroy, et al. 2024. “Whole Transcriptome Analysis Highlights Nutrient Limitation of Nitrogen Cycle Bacteria in Simulated Microgravity.” NPJ MICROGRAVITY 10 (1). https://doi.org/10.1038/s41526-024-00345-z.
Chicago author-date (all authors)
Verbeelen, Tom, Celia Alvarez Fernandez, Thanh Huy Nguyen, Surya Gupta, Raf Aarts, Kevin Tabury, Baptiste Leroy, Ruddy Wattiez, Siegfried E. Vlaeminck, Natalie Leys, Ramon Ganigué, and Felice Mastroleo. 2024. “Whole Transcriptome Analysis Highlights Nutrient Limitation of Nitrogen Cycle Bacteria in Simulated Microgravity.” NPJ MICROGRAVITY 10 (1). doi:10.1038/s41526-024-00345-z.
Vancouver
1.
Verbeelen T, Alvarez Fernandez C, Nguyen TH, Gupta S, Aarts R, Tabury K, et al. Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity. NPJ MICROGRAVITY. 2024;10(1).
IEEE
[1]
T. Verbeelen et al., “Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity,” NPJ MICROGRAVITY, vol. 10, no. 1, 2024.
@article{01HNMYGYKAA31M80SFGM4MTWVR,
  abstract     = {{Regenerative life support systems (RLSS) will play a vital role in achieving self-sufficiency during long-distance space travel. Urine conversion into a liquid nitrate-based fertilizer is a key process in most RLSS. This study describes the effects of simulated microgravity (SMG) on Comamonas testosteroni, Nitrosomonas europaea, Nitrobacter winogradskyi and a tripartite culture of the three, in the context of nitrogen recovery for the Micro-Ecological Life Support System Alternative (MELiSSA). Rotary cell culture systems (RCCS) and random positioning machines (RPM) were used as SMG analogues. The transcriptional responses of the cultures were elucidated. For CO2-producing C. testosteroni and the tripartite culture, a PermaLifeTM PL-70 cell culture bag mounted on an in-house 3D-printed holder was applied to eliminate air bubble formation during SMG cultivation. Gene expression changes indicated that the fluid dynamics in SMG caused nutrient and O2 limitation. Genes involved in urea hydrolysis and nitrification were minimally affected, while denitrification-related gene expression was increased. The findings highlight potential challenges for nitrogen recovery in space.}},
  articleno    = {{3}},
  author       = {{Verbeelen, Tom and Alvarez Fernandez, Celia and  Nguyen, Thanh Huy and  Gupta, Surya and  Aarts, Raf and  Tabury, Kevin and  Leroy, Baptiste and  Wattiez, Ruddy and  Vlaeminck, Siegfried E. and  Leys, Natalie and Ganigué, Ramon and  Mastroleo, Felice}},
  issn         = {{2373-8065}},
  journal      = {{NPJ MICROGRAVITY}},
  keywords     = {{SHEAR MODELED MICROGRAVITY,BIOFILM LIFE-STYLE,NITROSOMONAS-EUROPAEA,NITROBACTER-WINOGRADSKYI,OXIDATIVE STRESS,GENE-EXPRESSION,COMAMONAS-TESTOSTERONI,OXIDIZING BACTERIUM,GENOME SEQUENCE,GROWTH}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{19}},
  title        = {{Whole transcriptome analysis highlights nutrient limitation of nitrogen cycle bacteria in simulated microgravity}},
  url          = {{http://doi.org/10.1038/s41526-024-00345-z}},
  volume       = {{10}},
  year         = {{2024}},
}
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