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Ectopic expression of distinct PLC genes identifies 'compactness' as a possible architectural shoot strategy to cope with drought stress

Max van Hooren, Ringo van Wijk, Irina Vaseva (UGent) , Dominique Van Der Straeten (UGent) , Michel Haring and Teun Munnik
(2024) PLANT AND CELL PHYSIOLOGY. 65(6). p.885-903
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Abstract
Phospholipase C (PLC) has been implicated in several stress responses, including drought. Overexpression (OE) of PLC has been shown to improve drought tolerance in various plant species. Arabidopsis contains nine PLC genes, which are subdivided into four clades. Earlier, OE of PLC3, PLC5 or PLC7 was found to increase Arabidopsis' drought tolerance. Here, we confirm this for three other PLCs: PLC2, the only constitutively expressed AtPLC; PLC4, reported to have reduced salt tolerance and PLC9, of which the encoded enzyme was presumed to be catalytically inactive. To compare each PLC and to discover any other potential phenotype, two independent OE lines of six AtPLC genes, representing all four clades, were simultaneously monitored with the GROWSCREEN-FLUORO phenotyping platform, under both control- and mild-drought conditions. To investigate which tissues were most relevant to achieving drought survival, we additionally expressed AtPLC5 using 13 different cell- or tissue-specific promoters. While no significant differences in plant size, biomass or photosynthesis were found between PLC lines and wild-type (WT) plants, all PLC-OE lines, as well as those tissue-specific lines that promoted drought survival, exhibited a stronger decrease in 'convex hull perimeter' (= increase in 'compactness') under water deprivation compared to WT. Increased compactness has not been associated with drought or decreased water loss before although a hyponastic decrease in compactness in response to increased temperatures has been associated with water loss. We propose that the increased compactness could lead to decreased water loss and potentially provide a new breeding trait to select for drought tolerance.
Keywords
PHOSPHATIDYLINOSITOL-PHOSPHOLIPASE C2, ARABIDOPSIS-THALIANA, PHOSPHATIDIC-ACID, PHOSPHOINOSITIDE, TOLERANCE, RESPONSES, DYNAMICS, GROWTH, SALT, ACTIVATION, Arabidopsis, Compactness, Drought, Phenomics, Tissue-specific expression

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MLA
van Hooren, Max, et al. “Ectopic Expression of Distinct PLC Genes Identifies ‘compactness’ as a Possible Architectural Shoot Strategy to Cope with Drought Stress.” PLANT AND CELL PHYSIOLOGY, vol. 65, no. 6, 2024, pp. 885–903, doi:10.1093/pcp/pcad123.
APA
van Hooren, M., van Wijk, R., Vaseva, I., Van Der Straeten, D., Haring, M., & Munnik, T. (2024). Ectopic expression of distinct PLC genes identifies “compactness” as a possible architectural shoot strategy to cope with drought stress. PLANT AND CELL PHYSIOLOGY, 65(6), 885–903. https://doi.org/10.1093/pcp/pcad123
Chicago author-date
Hooren, Max van, Ringo van Wijk, Irina Vaseva, Dominique Van Der Straeten, Michel Haring, and Teun Munnik. 2024. “Ectopic Expression of Distinct PLC Genes Identifies ‘compactness’ as a Possible Architectural Shoot Strategy to Cope with Drought Stress.” PLANT AND CELL PHYSIOLOGY 65 (6): 885–903. https://doi.org/10.1093/pcp/pcad123.
Chicago author-date (all authors)
van Hooren, Max, Ringo van Wijk, Irina Vaseva, Dominique Van Der Straeten, Michel Haring, and Teun Munnik. 2024. “Ectopic Expression of Distinct PLC Genes Identifies ‘compactness’ as a Possible Architectural Shoot Strategy to Cope with Drought Stress.” PLANT AND CELL PHYSIOLOGY 65 (6): 885–903. doi:10.1093/pcp/pcad123.
Vancouver
1.
van Hooren M, van Wijk R, Vaseva I, Van Der Straeten D, Haring M, Munnik T. Ectopic expression of distinct PLC genes identifies “compactness” as a possible architectural shoot strategy to cope with drought stress. PLANT AND CELL PHYSIOLOGY. 2024;65(6):885–903.
IEEE
[1]
M. van Hooren, R. van Wijk, I. Vaseva, D. Van Der Straeten, M. Haring, and T. Munnik, “Ectopic expression of distinct PLC genes identifies ‘compactness’ as a possible architectural shoot strategy to cope with drought stress,” PLANT AND CELL PHYSIOLOGY, vol. 65, no. 6, pp. 885–903, 2024.
@article{01HNJ05X9E2HYS2FYMKFHSE9B0,
  abstract     = {{Phospholipase C (PLC) has been implicated in several stress responses, including drought. Overexpression (OE) of PLC has been shown to improve drought tolerance in various plant species. Arabidopsis contains nine PLC genes, which are subdivided into four clades. Earlier, OE of PLC3, PLC5 or PLC7 was found to increase Arabidopsis' drought tolerance. Here, we confirm this for three other PLCs: PLC2, the only constitutively expressed AtPLC; PLC4, reported to have reduced salt tolerance and PLC9, of which the encoded enzyme was presumed to be catalytically inactive. To compare each PLC and to discover any other potential phenotype, two independent OE lines of six AtPLC genes, representing all four clades, were simultaneously monitored with the GROWSCREEN-FLUORO phenotyping platform, under both control- and mild-drought conditions. To investigate which tissues were most relevant to achieving drought survival, we additionally expressed AtPLC5 using 13 different cell- or tissue-specific promoters. While no significant differences in plant size, biomass or photosynthesis were found between PLC lines and wild-type (WT) plants, all PLC-OE lines, as well as those tissue-specific lines that promoted drought survival, exhibited a stronger decrease in 'convex hull perimeter' (= increase in 'compactness') under water deprivation compared to WT. Increased compactness has not been associated with drought or decreased water loss before although a hyponastic decrease in compactness in response to increased temperatures has been associated with water loss. We propose that the increased compactness could lead to decreased water loss and potentially provide a new breeding trait to select for drought tolerance.}},
  author       = {{van Hooren, Max and  van Wijk, Ringo and Vaseva, Irina and Van Der Straeten, Dominique and  Haring, Michel and  Munnik, Teun}},
  issn         = {{0032-0781}},
  journal      = {{PLANT AND CELL PHYSIOLOGY}},
  keywords     = {{PHOSPHATIDYLINOSITOL-PHOSPHOLIPASE C2,ARABIDOPSIS-THALIANA,PHOSPHATIDIC-ACID,PHOSPHOINOSITIDE,TOLERANCE,RESPONSES,DYNAMICS,GROWTH,SALT,ACTIVATION,Arabidopsis,Compactness,Drought,Phenomics,Tissue-specific expression}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{885--903}},
  title        = {{Ectopic expression of distinct PLC genes identifies 'compactness' as a possible architectural shoot strategy to cope with drought stress}},
  url          = {{http://doi.org/10.1093/pcp/pcad123}},
  volume       = {{65}},
  year         = {{2024}},
}
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