@inproceedings{01HNF7YNM70WR08YSTWVEX3SN5,
  abstract     = {{Background
The use of blood-based extracellular RNA (cell-free RNA; exRNA) as clinical biomarker requires the implementation of a validated procedure for sample collection, processing, and profiling. So far, no study has systematically addressed the pre-analytical variables affecting transcriptome analysis of exRNAs. In the exRNAQC study, we evaluated ten blood collection tubes, three time intervals between blood draw and downstream processing, and eight RNA purification methods using the supplier-specified minimum and maximum biofluid input volumes. 

Methods
The impact of these pre-analytics on deep transcriptome profiling of both small and messenger RNA from healthy donors’ plasma or serum was assessed for each pre-analytical variable separately and for interactions between a selected set of pre-analytics, resulting in 456 extracellular transcriptomes. Making use of 189 synthetic spike-in RNAs, the processing and analysis workflow was controlled. 

Results
When comparing blood collection tubes, so-called preservation tubes do not stabilize exRNA well, and result in variable RNA concentration and sensitivity (i.e., the number of detected RNAs) over time, as well as compromised reproducibility. We also document large differences in RNA purification kit performance in terms of sensitivity, reproducibility, and observed transcriptome complexity, and demonstrate interactions between specific blood collection tubes, purification kits and time intervals. Our results are summarized in 11 performance metrics that enable an informed selection of the most optimal sample processing workflow for a given experiment. 

Conclusions
In conclusion, we put forward robust quality control metrics for exRNA quantification methods with validated standard operating procedures (SOPs), representing paramount groundwork for future exRNA-based precision medicine applications.

Abstract submitted on behalf of the exRNAQC Consortium. Authors are listed in alphabetical order.}},
  articleno    = {{O4.4}},
  author       = {{Anckaert, Jasper and Avila Cobos, Francisco and Decock, Anneleen and Decruyenaere, Philippe and Deleu, Jill and De Preter, Katleen and De Wever, Olivier and De Wilde, Jilke and Dhondt, Bert and D'huyvetter, Thibaut and Everaert, Celine and Fierro, Carolina and Helsmoortel, Hetty and Hendrix, An and Hulstaert, Eva and Koster, Jan and Kuersten, Scott and Mercer, Tim R and Mestdagh, Pieter and Morlion, Annelien and Nijs, Nele and Nuytens, Justine and Philippron, Annouck and Piofczyk, Thomas and Poma Soto, Franco Alexander and Schoofs, Kathleen and Schroth, Gary P. and Thas, Olivier and Vanden Eynde, Eveline and Vandesompele, Jo and Van Maerken, Tom and Van Paemel, Ruben and Verniers, Kimberly and Verwilt, Jasper and Yigit, Nurten}},
  booktitle    = {{6th ACTC Advances in Circulating Tumor Cells 'Liquid Biopsy and Precision Oncology : Where do we stand now?' Book of Abstracts}},
  language     = {{eng}},
  location     = {{Skiathos, Greece}},
  pages        = {{1}},
  title        = {{Dramatic impact of blood collection tube and RNA purification method on extracellular RNA transcriptomes}},
  url          = {{https://www.erasmus.gr/UsersFiles/microsite1261/Documents/ACTC2023_BOOKOFABSTRACTS_Final_updated.pdf}},
  year         = {{2023}},
}