Applications of nanopore sequencing for forensic analysis
- Author
- Olivier Tytgat and Filip Van Nieuwerburgh (UGent)
- Organization
- Abstract
- DNA sequencing for forensic purposes is currently almost exclusively performed by second-generation sequencing methods (Bruijns et al., 2018). Verogen and Thermo Fisher Scientific are among the most important providers, both relying on sequencing-by-synthesis (SBS). Verogen commercializes the MiSeq FGx Sequencing System, based on the Illumina platform. Thermo Fisher Scientific distributes the Ion Torrent systems (Churchill et al., 2016; Zhang et al., 2017). Both technologies yield high-accuracy reads at sufficient throughput. However, their widespread introduction into routine forensic use is hampered by some limitations. Firstly, SBS-based systems are short read sequencing systems, with a maximal read length of about 500 nucleotides, making assay design and read alignment of long short tandem repeats loci more challenging. Secondly, the acquisition of such a technology requires a large capital investment. Third, the cost per sample is pronouncedly higher compared to capillary electrophoresis. Moreover, highly trained staff is required for sample and library preparation. Lastly, analysis is performed in centralized laboratories.
- Keywords
- Forensic biology, Human genetics, Nanopore sequencing, Sequence analysis, Short tandem repeats
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HMRKYMXPQTQZ8VDWTB0CMWST
- MLA
- Tytgat, Olivier, and Filip Van Nieuwerburgh. “Applications of Nanopore Sequencing for Forensic Analysis.” Next Generation Sequencing (NGS) Technology in DNA Analysis, Academic Press, 2024, pp. 85–98, doi:10.1016/B978-0-323-99144-5.00027-5.
- APA
- Tytgat, O., & Van Nieuwerburgh, F. (2024). Applications of nanopore sequencing for forensic analysis. In Next generation sequencing (NGS) technology in DNA analysis (pp. 85–98). https://doi.org/10.1016/B978-0-323-99144-5.00027-5
- Chicago author-date
- Tytgat, Olivier, and Filip Van Nieuwerburgh. 2024. “Applications of Nanopore Sequencing for Forensic Analysis.” In Next Generation Sequencing (NGS) Technology in DNA Analysis, 85–98. Academic Press. https://doi.org/10.1016/B978-0-323-99144-5.00027-5.
- Chicago author-date (all authors)
- Tytgat, Olivier, and Filip Van Nieuwerburgh. 2024. “Applications of Nanopore Sequencing for Forensic Analysis.” In Next Generation Sequencing (NGS) Technology in DNA Analysis, 85–98. Academic Press. doi:10.1016/B978-0-323-99144-5.00027-5.
- Vancouver
- 1.Tytgat O, Van Nieuwerburgh F. Applications of nanopore sequencing for forensic analysis. In: Next generation sequencing (NGS) technology in DNA analysis. Academic Press; 2024. p. 85–98.
- IEEE
- [1]O. Tytgat and F. Van Nieuwerburgh, “Applications of nanopore sequencing for forensic analysis,” in Next generation sequencing (NGS) technology in DNA analysis, Academic Press, 2024, pp. 85–98.
@incollection{01HMRKYMXPQTQZ8VDWTB0CMWST,
abstract = {{DNA sequencing for forensic purposes is currently almost exclusively performed by second-generation sequencing methods (Bruijns et al., 2018). Verogen and Thermo Fisher Scientific are among the most important providers, both relying on sequencing-by-synthesis (SBS). Verogen commercializes the MiSeq FGx Sequencing System, based on the Illumina platform. Thermo Fisher Scientific distributes the Ion Torrent systems (Churchill et al., 2016; Zhang et al., 2017). Both technologies yield high-accuracy reads at sufficient throughput. However, their widespread introduction into routine forensic use is hampered by some limitations. Firstly, SBS-based systems are short read sequencing systems, with a maximal read length of about 500 nucleotides, making assay design and read alignment of long short tandem repeats loci more challenging. Secondly, the acquisition of such a technology requires a large capital investment. Third, the cost per sample is pronouncedly higher compared to capillary electrophoresis. Moreover, highly trained staff is required for sample and library preparation. Lastly, analysis is performed in centralized laboratories.}},
author = {{Tytgat, Olivier and Van Nieuwerburgh, Filip}},
booktitle = {{Next generation sequencing (NGS) technology in DNA analysis}},
isbn = {{9780323991445}},
keywords = {{Forensic biology,Human genetics,Nanopore sequencing,Sequence analysis,Short tandem repeats}},
language = {{eng}},
pages = {{85--98}},
publisher = {{Academic Press}},
title = {{Applications of nanopore sequencing for forensic analysis}},
url = {{http://doi.org/10.1016/B978-0-323-99144-5.00027-5}},
year = {{2024}},
}
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