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Transcriptome signature of immature and in vitro-matured equine Cumulus–Oocytes complex

Alejandro de la Fuente, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule (UGent) , Mats Troedsson, Peter Daels (UGent) , Stuart Meyers and Pouya Dini
(2023) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 24(18).
Author
Organization
Abstract
Maturation is a critical step in the development of an oocyte, and it is during this time that the oocyte advances to metaphase II (MII) of the meiotic cycle and acquires developmental competence to be fertilized and become an embryo. However, in vitro maturation (IVM) remains one of the limiting steps in the in vitro production of embryos (IVP), with a variable percentage of oocytes reaching the MII stage and unpredictable levels of developmental competence. Understanding the dynamics of oocyte maturation is essential for the optimization of IVM culture conditions and subsequent IVP outcomes. Thus, the aim of this study was to elucidate the transcriptome dynamics of oocyte maturation by comparing transcriptomic changes during in vitro maturation in both oocytes and their surrounding cumulus cells. Cumulus-oocyte complexes were obtained from antral follicles and divided into two groups: immature and in vitro-matured (MII). RNA was extracted separately from oocytes (OC) and cumulus cells (CC), followed by library preparation and RNA sequencing. A total of 13,918 gene transcripts were identified in OC, with 538 differentially expressed genes (DEG) between immature OC and in vitro-matured OC. In CC, 13,104 genes were expressed with 871 DEG. Gene ontology (GO) analysis showed an association between the DEGs and pathways relating to nuclear maturation in OC and GTPase activity, extracellular matrix organization, and collagen trimers in CC. Additionally, the follicle-stimulating hormone receptor gene (FSHR) and luteinizing hormone/choriogonadotropin receptor gene (LHCGR) showed differential expressions between CC-MII and immature CC samples. Overall, these results serve as a foundation to further investigate the biological pathways relevant to oocyte maturation in horses and pave the road to improve the IVP outcomes and the overall clinical management of equine assisted reproductive technologies (ART).
Keywords
Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis, oocyte, cumulus cell, in vitro embryo production, in vitro oocyte maturation, equine, transcriptome, ANTI-MULLERIAN HORMONE, INTRACYTOPLASMIC SPERM INJECTION, HYALURONIC-ACID SYNTHESIS, MOUSE OVARIAN-FOLLICLES, MATRIX METALLOPROTEINASES, GRANULOSA-CELLS, GAP-JUNCTIONS, TISSUE INHIBITORS, CYCLIC-GMP, IN-VIVO

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MLA
de la Fuente, Alejandro, et al. “Transcriptome Signature of Immature and in Vitro-Matured Equine Cumulus–Oocytes Complex.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 18, MDPI, 2023, doi:10.3390/ijms241813718.
APA
de la Fuente, A., Scoggin, C., Bradecamp, E., Martin-Pelaez, S., van Heule, M., Troedsson, M., … Dini, P. (2023). Transcriptome signature of immature and in vitro-matured equine Cumulus–Oocytes complex. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(18). https://doi.org/10.3390/ijms241813718
Chicago author-date
Fuente, Alejandro de la, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule, Mats Troedsson, Peter Daels, Stuart Meyers, and Pouya Dini. 2023. “Transcriptome Signature of Immature and in Vitro-Matured Equine Cumulus–Oocytes Complex.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (18). https://doi.org/10.3390/ijms241813718.
Chicago author-date (all authors)
de la Fuente, Alejandro, Charles Scoggin, Etta Bradecamp, Soledad Martin-Pelaez, Machteld van Heule, Mats Troedsson, Peter Daels, Stuart Meyers, and Pouya Dini. 2023. “Transcriptome Signature of Immature and in Vitro-Matured Equine Cumulus–Oocytes Complex.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (18). doi:10.3390/ijms241813718.
Vancouver
1.
de la Fuente A, Scoggin C, Bradecamp E, Martin-Pelaez S, van Heule M, Troedsson M, et al. Transcriptome signature of immature and in vitro-matured equine Cumulus–Oocytes complex. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2023;24(18).
IEEE
[1]
A. de la Fuente et al., “Transcriptome signature of immature and in vitro-matured equine Cumulus–Oocytes complex,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 18, 2023.
@article{01HMDQP4JMEGS7WZWRSFWFYSH5,
  abstract     = {{Maturation is a critical step in the development of an oocyte, and it is during this time that the oocyte advances to metaphase II (MII) of the meiotic cycle and acquires developmental competence to be fertilized and become an embryo. However, in vitro maturation (IVM) remains one of the limiting steps in the in vitro production of embryos (IVP), with a variable percentage of oocytes reaching the MII stage and unpredictable levels of developmental competence. Understanding the dynamics of oocyte maturation is essential for the optimization of IVM culture conditions and subsequent IVP outcomes. Thus, the aim of this study was to elucidate the transcriptome dynamics of oocyte maturation by comparing transcriptomic changes during in vitro maturation in both oocytes and their surrounding cumulus cells. Cumulus-oocyte complexes were obtained from antral follicles and divided into two groups: immature and in vitro-matured (MII). RNA was extracted separately from oocytes (OC) and cumulus cells (CC), followed by library preparation and RNA sequencing. A total of 13,918 gene transcripts were identified in OC, with 538 differentially expressed genes (DEG) between immature OC and in vitro-matured OC. In CC, 13,104 genes were expressed with 871 DEG. Gene ontology (GO) analysis showed an association between the DEGs and pathways relating to nuclear maturation in OC and GTPase activity, extracellular matrix organization, and collagen trimers in CC. Additionally, the follicle-stimulating hormone receptor gene (FSHR) and luteinizing hormone/choriogonadotropin receptor gene (LHCGR) showed differential expressions between CC-MII and immature CC samples. Overall, these results serve as a foundation to further investigate the biological pathways relevant to oocyte maturation in horses and pave the road to improve the IVP outcomes and the overall clinical management of equine assisted reproductive technologies (ART).}},
  articleno    = {{13718}},
  author       = {{de la Fuente, Alejandro and Scoggin, Charles and Bradecamp, Etta and Martin-Pelaez, Soledad and van Heule, Machteld and Troedsson, Mats and Daels, Peter and Meyers, Stuart and Dini, Pouya}},
  issn         = {{1661-6596}},
  journal      = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
  keywords     = {{Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis,oocyte,cumulus cell,in vitro embryo production,in vitro oocyte maturation,equine,transcriptome,ANTI-MULLERIAN HORMONE,INTRACYTOPLASMIC SPERM INJECTION,HYALURONIC-ACID SYNTHESIS,MOUSE OVARIAN-FOLLICLES,MATRIX METALLOPROTEINASES,GRANULOSA-CELLS,GAP-JUNCTIONS,TISSUE INHIBITORS,CYCLIC-GMP,IN-VIVO}},
  language     = {{eng}},
  number       = {{18}},
  pages        = {{15}},
  publisher    = {{MDPI}},
  title        = {{Transcriptome signature of immature and in vitro-matured equine Cumulus–Oocytes complex}},
  url          = {{http://doi.org/10.3390/ijms241813718}},
  volume       = {{24}},
  year         = {{2023}},
}
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