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Blood plasma and FFPE derived total RNA seq dataset from DLBCL and PMBCL patients

Philippe Decruyenaere (UGent) , Jo Vandesompele (UGent) and Fritz Offner (UGent)
(2023)
Author
Organization
Project
Abstract
The goal of this study was to investigate cell-free RNA (cfRNA) biomarkers in DLBCL and PMBCL patients. Blood plasma samples (n=168) and matched diagnostic formalin-fixed paraffin-embedded (FFPE) tissue samples (n=69) of DLBCL patients, PMBCL patients and healthy controls were collected between 2016-2021. Plasma samples were collected at diagnosis, at interim evaluation, after treatment, and in case of refractory or relapsed disease. RNA was extracted from 200 µl plasma using the miRNeasy serum/plasma kit and from FFPE tissue using the miRNeasy FFPE kit. RNA was subsequently sequenced on a NovaSeq 6000 instrument using the SMARTer Stranded Total RNA-seq pico v3 library preparation kit.
Keywords
Illumina NovaSeq 6000
License
CC-BY-NC-ND-4.0
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restricted access

Citation

Please use this url to cite or link to this publication: 

@misc{01HMBT3C9T83GSRBY7YY9JEEXT,
  abstract     = {{The goal of this study was to investigate cell-free RNA (cfRNA) biomarkers in DLBCL and PMBCL patients. Blood plasma samples (n=168) and matched diagnostic formalin-fixed paraffin-embedded (FFPE) tissue samples (n=69) of DLBCL patients, PMBCL patients and healthy controls were collected between 2016-2021. Plasma samples were collected at diagnosis, at interim evaluation, after treatment, and in case of refractory or relapsed disease. RNA was extracted from 200 µl plasma using the miRNeasy serum/plasma kit and from FFPE tissue using the miRNeasy FFPE kit. RNA was subsequently sequenced on a NovaSeq 6000 instrument using the SMARTer Stranded Total RNA-seq pico v3 library preparation kit.}},
  author       = {{Decruyenaere, Philippe and Vandesompele, Jo and Offner, Fritz}},
  keywords     = {{Illumina NovaSeq 6000}},
  language     = {{eng}},
  publisher    = {{European Genome-Phenome Archive}},
  title        = {{Blood plasma and FFPE derived total RNA seq dataset from DLBCL and PMBCL patients}},
  year         = {{2023}},
}