Contrasting roles of cytochrome P450s in amitraz and chlorfenapyr resistance in the crop pest Tetranychus urticae
- Author
- Marilou Vandenhole (UGent) , Xueping Lu (UGent) , Dimitra Tsakireli, Catherine Mermans (UGent) , Sander De Rouck (UGent) , Berdien De Beer, Eba Alemayehu Simma, Spiros A. Pergantis, Wim Jonckheere (UGent) , John Vontas and Thomas Van Leeuwen (UGent)
- Organization
- Project
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- SuperPests (Innovative tools for rational control of the most difficult-to-manage pests (super pests) and the diseases they transmit)
- POLYADAPT (Molecular-genetic mechanisms of extreme adaptation in a polyphagous agricultural pest)
- Mi(gh)ty CRISPR: developing CRISPR/Cas9 as a functional genetic tool for spider mite research
- Abstract
- The molecular mechanisms of amitraz and chlorfenapyr resistance remain only poorly understood for major agricultural pests and vectors of human diseases. This study focusses on a multi-resistant field strain of the crop pest Tetranychus urticae, which could be readily selected in the laboratory to high levels of amitraz and chlorfenapyr resistance. Toxicity experiments using tralopyril, the active toxophore of chlorfenapyr, suggested decreased activation as a likely mechanism underlying resistance. Starting from the same parental strain, transcriptome profiling revealed that a cluster of detoxifying genes was upregulated after amitraz selection, but unexpectedly downregulated after chlorfenapyr selection. Further functional validation associated the upregulation of CYP392A16 with amitraz metabolism and the downregulation of CYP392D8 with reduced activation of chlorfenapyr to tralopyril. Genetic mapping (QTL analysis by BSA) was conducted in an attempt to unravel the genetic mechanisms of expression variation and resistance. This revealed that chlorfenapyr resistance was associated with a single QTL, while 3 QTLs were uncovered for amitraz resistance. Together with the observed contrasting gene expression patterns, we argue that transcriptional regulators most likely underly the distinct expression profiles associated with resistance, but these await further functional validation.
- Keywords
- SPIDER-MITE ACARI, INCREASED SUSCEPTIBILITY, INSECTICIDE RESISTANCE, BIOCHEMICAL-ANALYSIS, BOOPHILUS-MICROPLUS, NEW-CALEDONIA, MODE, POPULATIONS, STRAIN, GENE, Gene regulation, Acaricide resistance, Detoxification, Genetic mapping, Mitochondrial uncouplers, Octopamine receptor, Spider mite
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HM97E46E4422286FYCZAFRQM
- MLA
- Vandenhole, Marilou, et al. “Contrasting Roles of Cytochrome P450s in Amitraz and Chlorfenapyr Resistance in the Crop Pest Tetranychus Urticae.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 164, 2024, doi:10.1016/j.ibmb.2023.104039.
- APA
- Vandenhole, M., Lu, X., Tsakireli, D., Mermans, C., De Rouck, S., De Beer, B., … Van Leeuwen, T. (2024). Contrasting roles of cytochrome P450s in amitraz and chlorfenapyr resistance in the crop pest Tetranychus urticae. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 164. https://doi.org/10.1016/j.ibmb.2023.104039
- Chicago author-date
- Vandenhole, Marilou, Xueping Lu, Dimitra Tsakireli, Catherine Mermans, Sander De Rouck, Berdien De Beer, Eba Alemayehu Simma, et al. 2024. “Contrasting Roles of Cytochrome P450s in Amitraz and Chlorfenapyr Resistance in the Crop Pest Tetranychus Urticae.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 164. https://doi.org/10.1016/j.ibmb.2023.104039.
- Chicago author-date (all authors)
- Vandenhole, Marilou, Xueping Lu, Dimitra Tsakireli, Catherine Mermans, Sander De Rouck, Berdien De Beer, Eba Alemayehu Simma, Spiros A. Pergantis, Wim Jonckheere, John Vontas, and Thomas Van Leeuwen. 2024. “Contrasting Roles of Cytochrome P450s in Amitraz and Chlorfenapyr Resistance in the Crop Pest Tetranychus Urticae.” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 164. doi:10.1016/j.ibmb.2023.104039.
- Vancouver
- 1.Vandenhole M, Lu X, Tsakireli D, Mermans C, De Rouck S, De Beer B, et al. Contrasting roles of cytochrome P450s in amitraz and chlorfenapyr resistance in the crop pest Tetranychus urticae. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY. 2024;164.
- IEEE
- [1]M. Vandenhole et al., “Contrasting roles of cytochrome P450s in amitraz and chlorfenapyr resistance in the crop pest Tetranychus urticae,” INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 164, 2024.
@article{01HM97E46E4422286FYCZAFRQM,
abstract = {{The molecular mechanisms of amitraz and chlorfenapyr resistance remain only poorly understood for major agricultural pests and vectors of human diseases. This study focusses on a multi-resistant field strain of the crop pest Tetranychus urticae, which could be readily selected in the laboratory to high levels of amitraz and chlorfenapyr resistance. Toxicity experiments using tralopyril, the active toxophore of chlorfenapyr, suggested decreased activation as a likely mechanism underlying resistance. Starting from the same parental strain, transcriptome profiling revealed that a cluster of detoxifying genes was upregulated after amitraz selection, but unexpectedly downregulated after chlorfenapyr selection. Further functional validation associated the upregulation of CYP392A16 with amitraz metabolism and the downregulation of CYP392D8 with reduced activation of chlorfenapyr to tralopyril. Genetic mapping (QTL analysis by BSA) was conducted in an attempt to unravel the genetic mechanisms of expression variation and resistance. This revealed that chlorfenapyr resistance was associated with a single QTL, while 3 QTLs were uncovered for amitraz resistance. Together with the observed contrasting gene expression patterns, we argue that transcriptional regulators most likely underly the distinct expression profiles associated with resistance, but these await further functional validation.}},
articleno = {{104039}},
author = {{Vandenhole, Marilou and Lu, Xueping and Tsakireli, Dimitra and Mermans, Catherine and De Rouck, Sander and De Beer, Berdien and Simma, Eba Alemayehu and Pergantis, Spiros A. and Jonckheere, Wim and Vontas, John and Van Leeuwen, Thomas}},
issn = {{0965-1748}},
journal = {{INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY}},
keywords = {{SPIDER-MITE ACARI,INCREASED SUSCEPTIBILITY,INSECTICIDE RESISTANCE,BIOCHEMICAL-ANALYSIS,BOOPHILUS-MICROPLUS,NEW-CALEDONIA,MODE,POPULATIONS,STRAIN,GENE,Gene regulation,Acaricide resistance,Detoxification,Genetic mapping,Mitochondrial uncouplers,Octopamine receptor,Spider mite}},
language = {{eng}},
pages = {{17}},
title = {{Contrasting roles of cytochrome P450s in amitraz and chlorfenapyr resistance in the crop pest Tetranychus urticae}},
url = {{http://doi.org/10.1016/j.ibmb.2023.104039}},
volume = {{164}},
year = {{2024}},
}
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