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Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori

Javad Sahandi, Patrick Sorgeloos (UGent) , Kam W. Tang, Fanghong Mu, Tatyana Mayor and Wenbing Zhang
(2023) MICROBIAL PATHOGENESIS. 183.
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Abstract
The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum, and Bacillus subtilis, to suppress Vibrio and increase survival, population growth and digestive enzyme activity (protease, lipase, and amylase) in the harpacticoid copepod, Tigriopus japonicus. Copepod, T. japonicus stock culture with an initial mean density of 50 individual/mL (25 adult male and 25 adult female) was distributed into five treatments (i.e., four experimental and a control, each with four replicates; repeated twice) using 20 beakers (100 mL capacity each). The copepods were fed a mixture of the dinoflagellate Alexandrium tamarense and the diatom Phyaeodactylum tricornutum (3 x 10(4) cells/mL(-1)). Each microbe's concentration was adjusted at 10(8) CFU/mL(-1 )and applied to the culture condition. D. hansenii, L. plantarum, and B. subtilis all improved the copepods' survival and population growth, likely by including a higher lipase activity (P < 0.05). In contrast, using R. mobilis did not improve the copepod's culture performance compared to control. B. subtilis was the most effective in decreasing the copepod's external and internal Vibrio loading. The probiotic concentrations in the copepod decreased within days during starvation, suggesting that routine re-application of the probiotics would be needed to sustain the microbial populations and the benefits they provide. Our results demonstrated that D. hansenii and B. subtilis are promising probiotics for mass copepod culture as live food for mariculture purposes.
Keywords
ACARTIA-TONSA, INTESTINAL MICROFLORA, HARPACTICOID COPEPODS, FRESH-WATER, FOOD, LARVAL, ZOOPLANKTON, BACTERIA, GROWTH, CULTIVATION, Bacillus subtilis, Copepod, Debaryomyces hansenii, Larvae, Microbe, Tigriopus japonicus

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Citation

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MLA
Sahandi, Javad, et al. “Beneficial Microbes to Suppress Vibrio and Improve the Culture Performance of Copepod Tigriopus Japonicus Mori.” MICROBIAL PATHOGENESIS, vol. 183, 2023, doi:10.1016/j.micpath.2023.106334.
APA
Sahandi, J., Sorgeloos, P., Tang, K. W., Mu, F., Mayor, T., & Zhang, W. (2023). Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori. MICROBIAL PATHOGENESIS, 183. https://doi.org/10.1016/j.micpath.2023.106334
Chicago author-date
Sahandi, Javad, Patrick Sorgeloos, Kam W. Tang, Fanghong Mu, Tatyana Mayor, and Wenbing Zhang. 2023. “Beneficial Microbes to Suppress Vibrio and Improve the Culture Performance of Copepod Tigriopus Japonicus Mori.” MICROBIAL PATHOGENESIS 183. https://doi.org/10.1016/j.micpath.2023.106334.
Chicago author-date (all authors)
Sahandi, Javad, Patrick Sorgeloos, Kam W. Tang, Fanghong Mu, Tatyana Mayor, and Wenbing Zhang. 2023. “Beneficial Microbes to Suppress Vibrio and Improve the Culture Performance of Copepod Tigriopus Japonicus Mori.” MICROBIAL PATHOGENESIS 183. doi:10.1016/j.micpath.2023.106334.
Vancouver
1.
Sahandi J, Sorgeloos P, Tang KW, Mu F, Mayor T, Zhang W. Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori. MICROBIAL PATHOGENESIS. 2023;183.
IEEE
[1]
J. Sahandi, P. Sorgeloos, K. W. Tang, F. Mu, T. Mayor, and W. Zhang, “Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori,” MICROBIAL PATHOGENESIS, vol. 183, 2023.
@article{01HM8NR2AHPXTMF78SZGD1TV5W,
  abstract     = {{The use of beneficial microbes, i.e., probiotics, to reduce pathogens and promote the performance of the target species is an important management strategy in mariculture. This study aimed to investigate the potential of four microbes, Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum, and Bacillus subtilis, to suppress Vibrio and increase survival, population growth and digestive enzyme activity (protease, lipase, and amylase) in the harpacticoid copepod, Tigriopus japonicus. Copepod, T. japonicus stock culture with an initial mean density of 50 individual/mL (25 adult male and 25 adult female) was distributed into five treatments (i.e., four experimental and a control, each with four replicates; repeated twice) using 20 beakers (100 mL capacity each). The copepods were fed a mixture of the dinoflagellate Alexandrium tamarense and the diatom Phyaeodactylum tricornutum (3 x 10(4) cells/mL(-1)). Each microbe's concentration was adjusted at 10(8) CFU/mL(-1 )and applied to the culture condition. D. hansenii, L. plantarum, and B. subtilis all improved the copepods' survival and population growth, likely by including a higher lipase activity (P < 0.05). In contrast, using R. mobilis did not improve the copepod's culture performance compared to control. B. subtilis was the most effective in decreasing the copepod's external and internal Vibrio loading. The probiotic concentrations in the copepod decreased within days during starvation, suggesting that routine re-application of the probiotics would be needed to sustain the microbial populations and the benefits they provide. Our results demonstrated that D. hansenii and B. subtilis are promising probiotics for mass copepod culture as live food for mariculture purposes.}},
  articleno    = {{106334}},
  author       = {{Sahandi, Javad and Sorgeloos, Patrick and  Tang, Kam W. and  Mu, Fanghong and  Mayor, Tatyana and  Zhang, Wenbing}},
  issn         = {{0882-4010}},
  journal      = {{MICROBIAL PATHOGENESIS}},
  keywords     = {{ACARTIA-TONSA,INTESTINAL MICROFLORA,HARPACTICOID COPEPODS,FRESH-WATER,FOOD,LARVAL,ZOOPLANKTON,BACTERIA,GROWTH,CULTIVATION,Bacillus subtilis,Copepod,Debaryomyces hansenii,Larvae,Microbe,Tigriopus japonicus}},
  language     = {{eng}},
  pages        = {{9}},
  title        = {{Beneficial microbes to suppress Vibrio and improve the culture performance of copepod Tigriopus japonicus Mori}},
  url          = {{http://doi.org/10.1016/j.micpath.2023.106334}},
  volume       = {{183}},
  year         = {{2023}},
}
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