Quantitative measurements of biochemical and molecular markers of oxidative stress signaling and responses
- Author
- Graham Noctor, Mathias Cohen (UGent) , Lug Trémulot, Frank Van Breusegem (UGent) and Amna M'Hamdi (UGent)
- Organization
- Abstract
- Increases in cellular oxidation are a part of most plant responses to challenging conditions and are commonly described as oxidative stress. While this phenomenon is closely related to the accumulation of reactive oxygen species, these latter compounds can be difficult to measure. Complementary measurements to assess cellular redox state are, therefore, very useful in studies of plant responses to stress. Here, we detail protocols for three complementary approaches that can be used to assess the intensity of oxidative stress. These involve quantification of marker transcripts, assays of the extractable activities of major antioxidative enzymes, and measurement of antioxidant buffers. We confirm experimentally that the data obtained by such approaches can provide reliable information on the intensity of oxidative stress.
- Keywords
- Antioxidants, Ascorbate, Ascorbate peroxidase, Catalase, Dehydroascorbate reductase, Glutathione, Glutathione reductase, Marker transcript, Redox state, UDP glycosyl transferase
Downloads
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 366.58 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HKW5TX9AD98HNPFESGY2C94K
- MLA
- Noctor, Graham, et al. “Quantitative Measurements of Biochemical and Molecular Markers of Oxidative Stress Signaling and Responses.” Plant Abiotic Stress Signaling, edited by Ivan Couée, vol. 2642, Humana, 2023, pp. 197–214, doi:10.1007/978-1-0716-3044-0_11.
- APA
- Noctor, G., Cohen, M., Trémulot, L., Van Breusegem, F., & M’Hamdi, A. (2023). Quantitative measurements of biochemical and molecular markers of oxidative stress signaling and responses. In I. Couée (Ed.), Plant abiotic stress signaling (Vol. 2642, pp. 197–214). https://doi.org/10.1007/978-1-0716-3044-0_11
- Chicago author-date
- Noctor, Graham, Mathias Cohen, Lug Trémulot, Frank Van Breusegem, and Amna M’Hamdi. 2023. “Quantitative Measurements of Biochemical and Molecular Markers of Oxidative Stress Signaling and Responses.” In Plant Abiotic Stress Signaling, edited by Ivan Couée, 2642:197–214. New York, NY, USA: Humana. https://doi.org/10.1007/978-1-0716-3044-0_11.
- Chicago author-date (all authors)
- Noctor, Graham, Mathias Cohen, Lug Trémulot, Frank Van Breusegem, and Amna M’Hamdi. 2023. “Quantitative Measurements of Biochemical and Molecular Markers of Oxidative Stress Signaling and Responses.” In Plant Abiotic Stress Signaling, ed by. Ivan Couée, 2642:197–214. New York, NY, USA: Humana. doi:10.1007/978-1-0716-3044-0_11.
- Vancouver
- 1.Noctor G, Cohen M, Trémulot L, Van Breusegem F, M’Hamdi A. Quantitative measurements of biochemical and molecular markers of oxidative stress signaling and responses. In: Couée I, editor. Plant abiotic stress signaling. New York, NY, USA: Humana; 2023. p. 197–214.
- IEEE
- [1]G. Noctor, M. Cohen, L. Trémulot, F. Van Breusegem, and A. M’Hamdi, “Quantitative measurements of biochemical and molecular markers of oxidative stress signaling and responses,” in Plant abiotic stress signaling, vol. 2642, I. Couée, Ed. New York, NY, USA: Humana, 2023, pp. 197–214.
@incollection{01HKW5TX9AD98HNPFESGY2C94K,
abstract = {{Increases in cellular oxidation are a part of most plant responses to challenging conditions and are commonly described as oxidative stress. While this phenomenon is closely related to the accumulation of reactive oxygen species, these latter compounds can be difficult to measure. Complementary measurements to assess cellular redox state are, therefore, very useful in studies of plant responses to stress. Here, we detail protocols for three complementary approaches that can be used to assess the intensity of oxidative stress. These involve quantification of marker transcripts, assays of the extractable activities of major antioxidative enzymes, and measurement of antioxidant buffers. We confirm experimentally that the data obtained by such approaches can provide reliable information on the intensity of oxidative stress.}},
author = {{Noctor, Graham and Cohen, Mathias and Trémulot, Lug and Van Breusegem, Frank and M'Hamdi, Amna}},
booktitle = {{Plant abiotic stress signaling}},
editor = {{Couée, Ivan}},
isbn = {{9781071630433}},
issn = {{1064-3745}},
keywords = {{Antioxidants,Ascorbate,Ascorbate peroxidase,Catalase,Dehydroascorbate reductase,Glutathione,Glutathione reductase,Marker transcript,Redox state,UDP glycosyl transferase}},
language = {{eng}},
pages = {{197--214}},
publisher = {{Humana}},
series = {{Methods in Molecular Biology}},
title = {{Quantitative measurements of biochemical and molecular markers of oxidative stress signaling and responses}},
url = {{http://doi.org/10.1007/978-1-0716-3044-0_11}},
volume = {{2642}},
year = {{2023}},
}
- Altmetric
- View in Altmetric