Age-dependent prebiotic effects of soluble corn fiber in M-SHIME® gut microbial ecosystems
- Author
- Marta Calatayud Arroyo (UGent) , Ieva Laurie, Chloe Rotsaert, Massimo Marzorati (UGent) , Davide Risso and Kavita Karnik
- Organization
- Abstract
- Soluble corn fiber (SCF) has demonstrated prebiotic effects in clinical studies. Using an in vitro mucosal simulator of the human intestinal microbial ecosystem (M-SHIME (R)) model, the effects of SCF treatment on colonic microbiota composition and metabolic activity and on host-microbiome interactions were evaluated using fecal samples from healthy donors of different ages (baby [<= 2 years], n = 4; adult [18-45 years], n = 2; elderly [70 years], n = 1). During the 3-week treatment period, M-SHIME (R) systems were supplemented with SCF daily (baby, 1.5, 3, or 4.5 g/d; adult, 3 or 8.5 g/d; and elderly, 8.5 g/d). M-SHIME (R) supernatants were evaluated for their effect on the intestinal epithelial cell barrier and inflammatory responses in lipopolysaccharide. (LPS)-stimulated cells. Additionally, short-chain fatty acid (SCFA) production and microbial community composition were assessed. In the baby and adult models, M-SHIME (R) supernatants from SCF treated vessels protected Caco-2 membrane integrity from LPS-induced damage. SCF treatment resulted in the expansion of Bacteroidetes, Firmicutes, and Bifidobacterial, as well as increased SCFA production in all age groups. SCF tended to have the greatest effect on propionate production. These findings demonstrate the prebiotic potential of SCF in babies, adults, and the elderly and provide insight into the mechanisms behind the observed prebiotic effects.
- Keywords
- Elderly, Infant, Microbiota, Prebiotic, Short-chain fatty acid, Soluble corn fiber, IN-VITRO, PROPIONATE, BUTYRATE, POLYDEXTROSE, INFLAMMATION, WEIGHT
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HKPWFWAETAPQRM95NC81P578
- MLA
- Calatayud Arroyo, Marta, et al. “Age-Dependent Prebiotic Effects of Soluble Corn Fiber in M-SHIME® Gut Microbial Ecosystems.” PLANT FOODS FOR HUMAN NUTRITION, vol. 78, no. 1, 2023, pp. 213–20, doi:10.1007/s11130-023-01043-z.
- APA
- Calatayud Arroyo, M., Laurie, I., Rotsaert, C., Marzorati, M., Risso, D., & Karnik, K. (2023). Age-dependent prebiotic effects of soluble corn fiber in M-SHIME® gut microbial ecosystems. PLANT FOODS FOR HUMAN NUTRITION, 78(1), 213–220. https://doi.org/10.1007/s11130-023-01043-z
- Chicago author-date
- Calatayud Arroyo, Marta, Ieva Laurie, Chloe Rotsaert, Massimo Marzorati, Davide Risso, and Kavita Karnik. 2023. “Age-Dependent Prebiotic Effects of Soluble Corn Fiber in M-SHIME® Gut Microbial Ecosystems.” PLANT FOODS FOR HUMAN NUTRITION 78 (1): 213–20. https://doi.org/10.1007/s11130-023-01043-z.
- Chicago author-date (all authors)
- Calatayud Arroyo, Marta, Ieva Laurie, Chloe Rotsaert, Massimo Marzorati, Davide Risso, and Kavita Karnik. 2023. “Age-Dependent Prebiotic Effects of Soluble Corn Fiber in M-SHIME® Gut Microbial Ecosystems.” PLANT FOODS FOR HUMAN NUTRITION 78 (1): 213–220. doi:10.1007/s11130-023-01043-z.
- Vancouver
- 1.Calatayud Arroyo M, Laurie I, Rotsaert C, Marzorati M, Risso D, Karnik K. Age-dependent prebiotic effects of soluble corn fiber in M-SHIME® gut microbial ecosystems. PLANT FOODS FOR HUMAN NUTRITION. 2023;78(1):213–20.
- IEEE
- [1]M. Calatayud Arroyo, I. Laurie, C. Rotsaert, M. Marzorati, D. Risso, and K. Karnik, “Age-dependent prebiotic effects of soluble corn fiber in M-SHIME® gut microbial ecosystems,” PLANT FOODS FOR HUMAN NUTRITION, vol. 78, no. 1, pp. 213–220, 2023.
@article{01HKPWFWAETAPQRM95NC81P578,
abstract = {{Soluble corn fiber (SCF) has demonstrated prebiotic effects in clinical studies. Using an in vitro mucosal simulator of the human intestinal microbial ecosystem (M-SHIME (R)) model, the effects of SCF treatment on colonic microbiota composition and metabolic activity and on host-microbiome interactions were evaluated using fecal samples from healthy donors of different ages (baby [<= 2 years], n = 4; adult [18-45 years], n = 2; elderly [70 years], n = 1). During the 3-week treatment period, M-SHIME (R) systems were supplemented with SCF daily (baby, 1.5, 3, or 4.5 g/d; adult, 3 or 8.5 g/d; and elderly, 8.5 g/d). M-SHIME (R) supernatants were evaluated for their effect on the intestinal epithelial cell barrier and inflammatory responses in lipopolysaccharide. (LPS)-stimulated cells. Additionally, short-chain fatty acid (SCFA) production and microbial community composition were assessed. In the baby and adult models, M-SHIME (R) supernatants from SCF treated vessels protected Caco-2 membrane integrity from LPS-induced damage. SCF treatment resulted in the expansion of Bacteroidetes, Firmicutes, and Bifidobacterial, as well as increased SCFA production in all age groups. SCF tended to have the greatest effect on propionate production. These findings demonstrate the prebiotic potential of SCF in babies, adults, and the elderly and provide insight into the mechanisms behind the observed prebiotic effects.}},
author = {{Calatayud Arroyo, Marta and Laurie, Ieva and Rotsaert, Chloe and Marzorati, Massimo and Risso, Davide and Karnik, Kavita}},
issn = {{0921-9668}},
journal = {{PLANT FOODS FOR HUMAN NUTRITION}},
keywords = {{Elderly,Infant,Microbiota,Prebiotic,Short-chain fatty acid,Soluble corn fiber,IN-VITRO,PROPIONATE,BUTYRATE,POLYDEXTROSE,INFLAMMATION,WEIGHT}},
language = {{eng}},
number = {{1}},
pages = {{213--220}},
title = {{Age-dependent prebiotic effects of soluble corn fiber in M-SHIME® gut microbial ecosystems}},
url = {{http://doi.org/10.1007/s11130-023-01043-z}},
volume = {{78}},
year = {{2023}},
}
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