A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing
- Author
- Ming Feng, Ai Zhang, Van Nguyen, Anchal Bisht, Curt Almqvist, Lieven De Veylder (UGent) , Annelie Carlsbecker and Charles W. Melnyk
- Organization
- Abstract
- The widespread use of plant grafting enables eudicots and gymnosperms to join with closely related species and grow as one. Gymnosperms have dominated forests for over 200 million years, and despite their economic and ecological relevance, we know little about how they graft. Here we developed a micrografting method in conifers using young tissues that allowed efficient grafting with closely related species and between distantly related genera. Conifer graft junctions rapidly connected vasculature and differentially expressed thousands of genes including auxin and cell-wall-related genes. By comparing these genes to those induced during Arabidopsis thaliana graft formation, we found a common activation of cambium, cell division, phloem and xylem-related genes. A gene regulatory network analysis in Norway spruce (Picea abies) predicted that PHYTOCHROME A SIGNAL TRANSDUCTION 1 (PAT1) acted as a core regulator of graft healing. This gene was strongly up-regulated during both spruce and Arabidopsis grafting, and Arabidopsis mutants lacking PAT genes failed to attach tissues or successfully graft. Complementing Arabidopsis PAT mutants with the spruce PAT1 homolog rescued tissue attachment and enhanced callus formation. Together, our data show an ability for young tissues to graft with distantly related species and identifies the PAT gene family as conserved regulators of graft healing and tissue regeneration.
- Keywords
- Plant Science
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HKKXKF6NE90011S07HJQ6ZC1
- MLA
- Feng, Ming, et al. “A Conserved Graft Formation Process in Norway Spruce and Arabidopsis Identifies the PAT Gene Family as Central Regulators of Wound Healing.” NATURE PLANTS, vol. 10, Springer Science and Business Media LLC, 2024, pp. 53–65, doi:10.1038/s41477-023-01568-w.
- APA
- Feng, M., Zhang, A., Nguyen, V., Bisht, A., Almqvist, C., De Veylder, L., … Melnyk, C. W. (2024). A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing. NATURE PLANTS, 10, 53–65. https://doi.org/10.1038/s41477-023-01568-w
- Chicago author-date
- Feng, Ming, Ai Zhang, Van Nguyen, Anchal Bisht, Curt Almqvist, Lieven De Veylder, Annelie Carlsbecker, and Charles W. Melnyk. 2024. “A Conserved Graft Formation Process in Norway Spruce and Arabidopsis Identifies the PAT Gene Family as Central Regulators of Wound Healing.” NATURE PLANTS 10: 53–65. https://doi.org/10.1038/s41477-023-01568-w.
- Chicago author-date (all authors)
- Feng, Ming, Ai Zhang, Van Nguyen, Anchal Bisht, Curt Almqvist, Lieven De Veylder, Annelie Carlsbecker, and Charles W. Melnyk. 2024. “A Conserved Graft Formation Process in Norway Spruce and Arabidopsis Identifies the PAT Gene Family as Central Regulators of Wound Healing.” NATURE PLANTS 10: 53–65. doi:10.1038/s41477-023-01568-w.
- Vancouver
- 1.Feng M, Zhang A, Nguyen V, Bisht A, Almqvist C, De Veylder L, et al. A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing. NATURE PLANTS. 2024;10:53–65.
- IEEE
- [1]M. Feng et al., “A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing,” NATURE PLANTS, vol. 10, pp. 53–65, 2024.
@article{01HKKXKF6NE90011S07HJQ6ZC1,
abstract = {{The widespread use of plant grafting enables eudicots and gymnosperms to join with closely related species and grow as one. Gymnosperms have dominated forests for over 200 million years, and despite their economic and ecological relevance, we know little about how they graft. Here we developed a micrografting method in conifers using young tissues that allowed efficient grafting with closely related species and between distantly related genera. Conifer graft junctions rapidly connected vasculature and differentially expressed thousands of genes including auxin and cell-wall-related genes. By comparing these genes to those induced during Arabidopsis thaliana graft formation, we found a common activation of cambium, cell division, phloem and xylem-related genes. A gene regulatory network analysis in Norway spruce (Picea abies) predicted that PHYTOCHROME A SIGNAL TRANSDUCTION 1 (PAT1) acted as a core regulator of graft healing. This gene was strongly up-regulated during both spruce and Arabidopsis grafting, and Arabidopsis mutants lacking PAT genes failed to attach tissues or successfully graft. Complementing Arabidopsis PAT mutants with the spruce PAT1 homolog rescued tissue attachment and enhanced callus formation. Together, our data show an ability for young tissues to graft with distantly related species and identifies the PAT gene family as conserved regulators of graft healing and tissue regeneration.}},
author = {{Feng, Ming and Zhang, Ai and Nguyen, Van and Bisht, Anchal and Almqvist, Curt and De Veylder, Lieven and Carlsbecker, Annelie and Melnyk, Charles W.}},
issn = {{2055-026X}},
journal = {{NATURE PLANTS}},
keywords = {{Plant Science}},
language = {{eng}},
pages = {{53--65}},
publisher = {{Springer Science and Business Media LLC}},
title = {{A conserved graft formation process in Norway spruce and Arabidopsis identifies the PAT gene family as central regulators of wound healing}},
url = {{http://doi.org/10.1038/s41477-023-01568-w}},
volume = {{10}},
year = {{2024}},
}
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