Opportunities in optical and electrical single-cell technologies to study microbial ecosystems
- Author
- Fabian Mermans (UGent) , Valérie Mattelin, Ruben van den Eeckhoudt, Cristina García Timermans (UGent) , Josefien Van Landuyt (UGent) , Yuting Guo, Irene Taurino, Filip Tavernier, Michael Kraft, Hira Khan (UGent) and Nico Boon (UGent)
- Organization
- Project
-
- Antiseptic induced oral microbial massacres: a fast-food feast for oral pathogens?
- Geavanceerde microbiële degradeerbaarheid van plastics door nieuwe enzyme polymeer-interacties
- BIOSTABLE: safe drinking water now and in the future
- From dawn till dusk: synthetic granule development for tailor-made microbial community engineering
- Microbial Chip to Capture, Analyse and Steer communities (MICCAS)
- Abstract
- New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions.
- Keywords
- single-cell, microbial ecology, optical techniques, electrical techniques, flow cytometry, Raman spectroscopy, CMOS-MEA, impedance flow cytometry, FLOW-CYTOMETRIC ANALYSIS, IN-SITU HYBRIDIZATION, DRINKING-WATER, RAMAN-SPECTROSCOPY, ESCHERICHIA-COLI, FLUORESCENCE MICROSCOPY, LIVING CELLS, CARD-FISH, BACTERIAL, MICROORGANISMS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HKCX7QA1CXWKSHXERX4JKHZ6
- MLA
- Mermans, Fabian, et al. “Opportunities in Optical and Electrical Single-Cell Technologies to Study Microbial Ecosystems.” FRONTIERS IN MICROBIOLOGY, vol. 14, 2023, doi:10.3389/fmicb.2023.1233705.
- APA
- Mermans, F., Mattelin, V., van den Eeckhoudt, R., García Timermans, C., Van Landuyt, J., Guo, Y., … Boon, N. (2023). Opportunities in optical and electrical single-cell technologies to study microbial ecosystems. FRONTIERS IN MICROBIOLOGY, 14. https://doi.org/10.3389/fmicb.2023.1233705
- Chicago author-date
- Mermans, Fabian, Valérie Mattelin, Ruben van den Eeckhoudt, Cristina García Timermans, Josefien Van Landuyt, Yuting Guo, Irene Taurino, et al. 2023. “Opportunities in Optical and Electrical Single-Cell Technologies to Study Microbial Ecosystems.” FRONTIERS IN MICROBIOLOGY 14. https://doi.org/10.3389/fmicb.2023.1233705.
- Chicago author-date (all authors)
- Mermans, Fabian, Valérie Mattelin, Ruben van den Eeckhoudt, Cristina García Timermans, Josefien Van Landuyt, Yuting Guo, Irene Taurino, Filip Tavernier, Michael Kraft, Hira Khan, and Nico Boon. 2023. “Opportunities in Optical and Electrical Single-Cell Technologies to Study Microbial Ecosystems.” FRONTIERS IN MICROBIOLOGY 14. doi:10.3389/fmicb.2023.1233705.
- Vancouver
- 1.Mermans F, Mattelin V, van den Eeckhoudt R, García Timermans C, Van Landuyt J, Guo Y, et al. Opportunities in optical and electrical single-cell technologies to study microbial ecosystems. FRONTIERS IN MICROBIOLOGY. 2023;14.
- IEEE
- [1]F. Mermans et al., “Opportunities in optical and electrical single-cell technologies to study microbial ecosystems,” FRONTIERS IN MICROBIOLOGY, vol. 14, 2023.
@article{01HKCX7QA1CXWKSHXERX4JKHZ6,
abstract = {{New techniques are revolutionizing single-cell research, allowing us to study microbes at unprecedented scales and in unparalleled depth. This review highlights the state-of-the-art technologies in single-cell analysis in microbial ecology applications, with particular attention to both optical tools, i.e., specialized use of flow cytometry and Raman spectroscopy and emerging electrical techniques. The objectives of this review include showcasing the diversity of single-cell optical approaches for studying microbiological phenomena, highlighting successful applications in understanding microbial systems, discussing emerging techniques, and encouraging the combination of established and novel approaches to address research questions. The review aims to answer key questions such as how single-cell approaches have advanced our understanding of individual and interacting cells, how they have been used to study uncultured microbes, which new analysis tools will become widespread, and how they contribute to our knowledge of ecological interactions.}},
articleno = {{1233705}},
author = {{Mermans, Fabian and Mattelin, Valérie and van den Eeckhoudt, Ruben and García Timermans, Cristina and Van Landuyt, Josefien and Guo, Yuting and Taurino, Irene and Tavernier, Filip and Kraft, Michael and Khan, Hira and Boon, Nico}},
issn = {{1664-302X}},
journal = {{FRONTIERS IN MICROBIOLOGY}},
keywords = {{single-cell,microbial ecology,optical techniques,electrical techniques,flow cytometry,Raman spectroscopy,CMOS-MEA,impedance flow cytometry,FLOW-CYTOMETRIC ANALYSIS,IN-SITU HYBRIDIZATION,DRINKING-WATER,RAMAN-SPECTROSCOPY,ESCHERICHIA-COLI,FLUORESCENCE MICROSCOPY,LIVING CELLS,CARD-FISH,BACTERIAL,MICROORGANISMS}},
language = {{eng}},
pages = {{22}},
title = {{Opportunities in optical and electrical single-cell technologies to study microbial ecosystems}},
url = {{http://doi.org/10.3389/fmicb.2023.1233705}},
volume = {{14}},
year = {{2023}},
}
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