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Validation of a targeted LC-MS/MS method for cereulide and application in food and faeces

(2024) TOXINS. 16(1).
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Abstract
Cereulide is an emetic toxin produced by some strains of Bacillus cereus. This bacterial toxin, a cyclic 1.2 kDa dodecadepsipeptide, is stable to heat and acids and causes nausea and vomiting when ingested via contaminated food. This work aimed to develop and validate a targeted analytical method applying liquid chromatography-tandem mass spectrometry (LC–MS/MS) to quantify this toxin in food and human faeces. Samples were extracted with acetonitrile in the presence of 13C6-cereulide, a labelled internal standard, and purified by centrifugation and filtration. The limits of quantification were 0.5 and 0.3 µg kg−1 for food and faeces, respectively. The linearity of the method was very good, with calculated R2 values above 0.995. The mean recovery of the method was within the acceptable range of 70.0%–120.0%, the repeatability was not higher than 7.3%, and the highest intra-laboratory reproducibility was 8.9%. The estimated range for the expanded measurement uncertainty was between 5.1% and 18.0%. The LC–MS/MS method was used to analyse one food sample (rice) from a Belgian foodborne outbreak and five faecal samples from patients with clinical symptoms after consumption of the contaminated rice. The levels of cereulide were 12.22 µg g−1 for food and between 6.32 and 773.37 ng g−1 for faecal samples.
Keywords
cereulide, LC-MS/MS, validation, food, faeces, bacterial toxin, EMETIC TOXIN CEREULIDE, BACILLUS-CEREUS, SYNTHETIC CEREULIDE, QUANTIFICATION, PRODUCTS, PASTA, DODECADEPSIPEPTIDE, OUTBREAK, ASSAY, RICE

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MLA
Masquelier, Julien, et al. “Validation of a Targeted LC-MS/MS Method for Cereulide and Application in Food and Faeces.” TOXINS, vol. 16, no. 1, 2024, doi:10.3390/toxins16010013.
APA
Masquelier, J., Segers, C., Jacobs, B., Van Nieuwenhuysen, T., Delbrassinne, L., & Van Hoeck, E. (2024). Validation of a targeted LC-MS/MS method for cereulide and application in food and faeces. TOXINS, 16(1). https://doi.org/10.3390/toxins16010013
Chicago author-date
Masquelier, Julien, Céline Segers, Bram Jacobs, Tom Van Nieuwenhuysen, Laurence Delbrassinne, and Els Van Hoeck. 2024. “Validation of a Targeted LC-MS/MS Method for Cereulide and Application in Food and Faeces.” TOXINS 16 (1). https://doi.org/10.3390/toxins16010013.
Chicago author-date (all authors)
Masquelier, Julien, Céline Segers, Bram Jacobs, Tom Van Nieuwenhuysen, Laurence Delbrassinne, and Els Van Hoeck. 2024. “Validation of a Targeted LC-MS/MS Method for Cereulide and Application in Food and Faeces.” TOXINS 16 (1). doi:10.3390/toxins16010013.
Vancouver
1.
Masquelier J, Segers C, Jacobs B, Van Nieuwenhuysen T, Delbrassinne L, Van Hoeck E. Validation of a targeted LC-MS/MS method for cereulide and application in food and faeces. TOXINS. 2024;16(1).
IEEE
[1]
J. Masquelier, C. Segers, B. Jacobs, T. Van Nieuwenhuysen, L. Delbrassinne, and E. Van Hoeck, “Validation of a targeted LC-MS/MS method for cereulide and application in food and faeces,” TOXINS, vol. 16, no. 1, 2024.
@article{01HK4J65PG316ZP8834FW5ZXDC,
  abstract     = {{Cereulide is an emetic toxin produced by some strains of Bacillus cereus. This bacterial toxin, a cyclic 1.2 kDa dodecadepsipeptide, is stable to heat and acids and causes nausea and vomiting when ingested via contaminated food. This work aimed to develop and validate a targeted analytical method applying liquid chromatography-tandem mass spectrometry (LC–MS/MS) to quantify this toxin in food and human faeces. Samples were extracted with acetonitrile in the presence of 13C6-cereulide, a labelled internal standard, and purified by centrifugation and filtration. The limits of quantification were 0.5 and 0.3 µg kg−1 for food and faeces, respectively. The linearity of the method was very good, with calculated R2 values above 0.995. The mean recovery of the method was within the acceptable range of 70.0%–120.0%, the repeatability was not higher than 7.3%, and the highest intra-laboratory reproducibility was 8.9%. The estimated range for the expanded measurement uncertainty was between 5.1% and 18.0%. The LC–MS/MS method was used to analyse one food sample (rice) from a Belgian foodborne outbreak and five faecal samples from patients with clinical symptoms after consumption of the contaminated rice. The levels of cereulide were 12.22 µg g−1 for food and between 6.32 and 773.37 ng g−1 for faecal samples.}},
  articleno    = {{13}},
  author       = {{Masquelier, Julien and Segers, Céline and Jacobs, Bram and Van Nieuwenhuysen, Tom and Delbrassinne, Laurence and Van Hoeck, Els}},
  issn         = {{2072-6651}},
  journal      = {{TOXINS}},
  keywords     = {{cereulide,LC-MS/MS,validation,food,faeces,bacterial toxin,EMETIC TOXIN CEREULIDE,BACILLUS-CEREUS,SYNTHETIC CEREULIDE,QUANTIFICATION,PRODUCTS,PASTA,DODECADEPSIPEPTIDE,OUTBREAK,ASSAY,RICE}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{13}},
  title        = {{Validation of a targeted LC-MS/MS method for cereulide and application in food and faeces}},
  url          = {{http://doi.org/10.3390/toxins16010013}},
  volume       = {{16}},
  year         = {{2024}},
}

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