Enhanced removal of warfare agent tri-nitro-toluene by a Methylophaga-dominated microbiome
- Author
- Kankana Kundu (UGent) , Josefien Van Landuyt (UGent) , Valérie Mattelin (UGent) , Bram Martin (UGent) , Marijke Neyts, Koen Parmentier and Nico Boon (UGent)
- Organization
- Project
-
- DISARM (Disseminating Innovative Solutions for Antibiotic Resistance Management - DISARM)
- Abstract
- Historical exposure of the marine environment to 2,4,6-trinitrotoluene (TNT) happened due to the dumping of left-over munitions. Despite significant research on TNT decontamination, the potential of marine microbiome for TNT degradation remains only little explored. In this study, TNT degradation experiments were conducted with sediment located near the World War I munition dumpsite - Paardenmarkt in the Belgian part of North Sea. A slow removal was observed using TNT as sole source of C and N, which could be enhanced by adding methanol. Degradation was reflected in nitro-reduced metabolites and microbial growth. 16S Illumina sequencing analysis revealed several enriched genera that used TNT as a sole source of C and N -Colwellia, Thalossospira, and Methylophaga. Addition of methanol resulted in increased abundance of Methylophaga, which corresponded to the rapid removal of TNT. Methanol enhanced the degradation by providing additional energy and establishing syntrophic association between methanol-utilizing and TNT-utilizing bacteria.
- Keywords
- Explosives, Tri -nitro -toluene, Degradation, Marine environment, Methylophaga, TNT-CONTAMINATED SOIL, BIOLOGICAL TREATMENT, 2,4,6-TRINITROTOLUENE, EXPLOSIVES, BIODEGRADATION, TRANSFORMATION, SEDIMENT, BACTERIA, WATER, HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE
Downloads
-
Enhanced removal - NBoon.pdf
- full text (Published version)
- |
- open access
- |
- |
- 4.57 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HJ14Q38WHV98C49GWD3JG2G8
- MLA
- Kundu, Kankana, et al. “Enhanced Removal of Warfare Agent Tri-Nitro-Toluene by a Methylophaga-Dominated Microbiome.” MARINE POLLUTION BULLETIN, vol. 190, 2023, doi:10.1016/j.marpolbul.2023.114866.
- APA
- Kundu, K., Van Landuyt, J., Mattelin, V., Martin, B., Neyts, M., Parmentier, K., & Boon, N. (2023). Enhanced removal of warfare agent tri-nitro-toluene by a Methylophaga-dominated microbiome. MARINE POLLUTION BULLETIN, 190. https://doi.org/10.1016/j.marpolbul.2023.114866
- Chicago author-date
- Kundu, Kankana, Josefien Van Landuyt, Valérie Mattelin, Bram Martin, Marijke Neyts, Koen Parmentier, and Nico Boon. 2023. “Enhanced Removal of Warfare Agent Tri-Nitro-Toluene by a Methylophaga-Dominated Microbiome.” MARINE POLLUTION BULLETIN 190. https://doi.org/10.1016/j.marpolbul.2023.114866.
- Chicago author-date (all authors)
- Kundu, Kankana, Josefien Van Landuyt, Valérie Mattelin, Bram Martin, Marijke Neyts, Koen Parmentier, and Nico Boon. 2023. “Enhanced Removal of Warfare Agent Tri-Nitro-Toluene by a Methylophaga-Dominated Microbiome.” MARINE POLLUTION BULLETIN 190. doi:10.1016/j.marpolbul.2023.114866.
- Vancouver
- 1.Kundu K, Van Landuyt J, Mattelin V, Martin B, Neyts M, Parmentier K, et al. Enhanced removal of warfare agent tri-nitro-toluene by a Methylophaga-dominated microbiome. MARINE POLLUTION BULLETIN. 2023;190.
- IEEE
- [1]K. Kundu et al., “Enhanced removal of warfare agent tri-nitro-toluene by a Methylophaga-dominated microbiome,” MARINE POLLUTION BULLETIN, vol. 190, 2023.
@article{01HJ14Q38WHV98C49GWD3JG2G8,
abstract = {{Historical exposure of the marine environment to 2,4,6-trinitrotoluene (TNT) happened due to the dumping of left-over munitions. Despite significant research on TNT decontamination, the potential of marine microbiome for TNT degradation remains only little explored. In this study, TNT degradation experiments were conducted with sediment located near the World War I munition dumpsite - Paardenmarkt in the Belgian part of North Sea. A slow removal was observed using TNT as sole source of C and N, which could be enhanced by adding methanol. Degradation was reflected in nitro-reduced metabolites and microbial growth. 16S Illumina sequencing analysis revealed several enriched genera that used TNT as a sole source of C and N -Colwellia, Thalossospira, and Methylophaga. Addition of methanol resulted in increased abundance of Methylophaga, which corresponded to the rapid removal of TNT. Methanol enhanced the degradation by providing additional energy and establishing syntrophic association between methanol-utilizing and TNT-utilizing bacteria.}},
articleno = {{114866}},
author = {{Kundu, Kankana and Van Landuyt, Josefien and Mattelin, Valérie and Martin, Bram and Neyts, Marijke and Parmentier, Koen and Boon, Nico}},
issn = {{0025-326X}},
journal = {{MARINE POLLUTION BULLETIN}},
keywords = {{Explosives,Tri -nitro -toluene,Degradation,Marine environment,Methylophaga,TNT-CONTAMINATED SOIL,BIOLOGICAL TREATMENT,2,4,6-TRINITROTOLUENE,EXPLOSIVES,BIODEGRADATION,TRANSFORMATION,SEDIMENT,BACTERIA,WATER,HEXAHYDRO-1,3,5-TRINITRO-1,3,5-TRIAZINE}},
language = {{eng}},
pages = {{10}},
title = {{Enhanced removal of warfare agent tri-nitro-toluene by a Methylophaga-dominated microbiome}},
url = {{http://doi.org/10.1016/j.marpolbul.2023.114866}},
volume = {{190}},
year = {{2023}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: