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2D fibrillar osteoid niche mimicry through inclusion of visco-elastic and topographical cues in gelatin-based networks

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Abstract
Given the clinical need for osteoregenerative materials incorporating controlled biomimetic and biophysical cues, a novel highly-substituted norbornene-modified gelatin was developed enabling thiol-ene crosslinking exploiting thiolated gelatin as cell-interactive crosslinker. Comparing the number of physical crosslinks, the degree of hydrolytic degradation upon modification, the network density and the chemical crosslinking type, the osteogenic effect of visco-elastic and topographical properties was evaluated. This novel network outperformed conventional gelatin-based networks in terms of osteogenesis induction, as evidenced in 2D dental pulp stem cell seeding assays, resulting from the presentation of both a local (substrate elasticity, 25-40 kPa) and a bulk (compressive modulus, 25-45 kPa) osteogenic substrate modulus in combination with adequate fibrillar cell adhesion spacing to optimally transfer traction forces from the fibrillar ECM (as evidenced by mesh size determination with the rubber elasticity theory) and resulting in a 1.7-fold increase in calcium production (compared to the gold standard gelatin methacryloyl (GelMA)).
Keywords
Mesenchymal stem cells, Modified gelatins, Biophysical cues, MESENCHYMAL STEM-CELLS, ADIPOSE-TISSUE, BONE-MARROW, IN-VITRO, SCAFFOLDS, HYDROGELS

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Citation

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MLA
Parmentier, Laurens, et al. “2D Fibrillar Osteoid Niche Mimicry through Inclusion of Visco-Elastic and Topographical Cues in Gelatin-Based Networks.” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 254, no. Part 1, 2024, doi:10.1016/j.ijbiomac.2023.127619.
APA
Parmentier, L., D’Haese, S., Duquesne, J., Bray, F., Van der Meeren, L., Skirtach, A., … Van Vlierberghe, S. (2024). 2D fibrillar osteoid niche mimicry through inclusion of visco-elastic and topographical cues in gelatin-based networks. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 254(Part 1). https://doi.org/10.1016/j.ijbiomac.2023.127619
Chicago author-date
Parmentier, Laurens, Sophie D’Haese, Jessie Duquesne, Fabrice Bray, Louis Van der Meeren, Andre Skirtach, Christian Rolando, Ruslan Dmitriev, and Sandra Van Vlierberghe. 2024. “2D Fibrillar Osteoid Niche Mimicry through Inclusion of Visco-Elastic and Topographical Cues in Gelatin-Based Networks.” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 254 (Part 1). https://doi.org/10.1016/j.ijbiomac.2023.127619.
Chicago author-date (all authors)
Parmentier, Laurens, Sophie D’Haese, Jessie Duquesne, Fabrice Bray, Louis Van der Meeren, Andre Skirtach, Christian Rolando, Ruslan Dmitriev, and Sandra Van Vlierberghe. 2024. “2D Fibrillar Osteoid Niche Mimicry through Inclusion of Visco-Elastic and Topographical Cues in Gelatin-Based Networks.” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 254 (Part 1). doi:10.1016/j.ijbiomac.2023.127619.
Vancouver
1.
Parmentier L, D’Haese S, Duquesne J, Bray F, Van der Meeren L, Skirtach A, et al. 2D fibrillar osteoid niche mimicry through inclusion of visco-elastic and topographical cues in gelatin-based networks. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 2024;254(Part 1).
IEEE
[1]
L. Parmentier et al., “2D fibrillar osteoid niche mimicry through inclusion of visco-elastic and topographical cues in gelatin-based networks,” INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol. 254, no. Part 1, 2024.
@article{01HJ13J7T17RCGV65JJR5F89A9,
  abstract     = {{Given the clinical need for osteoregenerative materials incorporating controlled biomimetic and biophysical cues, a novel highly-substituted norbornene-modified gelatin was developed enabling thiol-ene crosslinking exploiting thiolated gelatin as cell-interactive crosslinker. Comparing the number of physical crosslinks, the degree of hydrolytic degradation upon modification, the network density and the chemical crosslinking type, the osteogenic effect of visco-elastic and topographical properties was evaluated. This novel network outperformed conventional gelatin-based networks in terms of osteogenesis induction, as evidenced in 2D dental pulp stem cell seeding assays, resulting from the presentation of both a local (substrate elasticity, 25-40 kPa) and a bulk (compressive modulus, 25-45 kPa) osteogenic substrate modulus in combination with adequate fibrillar cell adhesion spacing to optimally transfer traction forces from the fibrillar ECM (as evidenced by mesh size determination with the rubber elasticity theory) and resulting in a 1.7-fold increase in calcium production (compared to the gold standard gelatin methacryloyl (GelMA)).}},
  articleno    = {{127619}},
  author       = {{Parmentier, Laurens and D'Haese, Sophie and Duquesne, Jessie and Bray, Fabrice and Van der Meeren, Louis and Skirtach, Andre and Rolando, Christian and Dmitriev, Ruslan and Van Vlierberghe, Sandra}},
  issn         = {{0141-8130}},
  journal      = {{INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES}},
  keywords     = {{Mesenchymal stem cells,Modified gelatins,Biophysical cues,MESENCHYMAL STEM-CELLS,ADIPOSE-TISSUE,BONE-MARROW,IN-VITRO,SCAFFOLDS,HYDROGELS}},
  language     = {{eng}},
  number       = {{Part 1}},
  pages        = {{13}},
  title        = {{2D fibrillar osteoid niche mimicry through inclusion of visco-elastic and topographical cues in gelatin-based networks}},
  url          = {{http://doi.org/10.1016/j.ijbiomac.2023.127619}},
  volume       = {{254}},
  year         = {{2024}},
}

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