Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 1.38 MB
Add to list
  1. Search results
  2. In vitro regeneration of immature zyg...

In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding

Priscilla N. Kimani, Lydia N. Wamalwa, Kahiu Ngugi, Stephen F. Omondi, Joseph M. Machua, Titus Magomere, Jackson Mulatya and Stefaan Werbrouck (UGent)
(2024) PLANT CELL TISSUE AND ORGAN CULTURE. 156(1).
Author
Organization
Abstract
Melia volkensii is a dryland mahogany tree species in East Africa that can be harvested for timber in 15 years and has potential for commercial planting in arid and semi-arid areas. The current breeding programme focusing on growth rate, growth habit and shape is delayed by the long juvenile period and slow seed development. This study evaluated early embryo regeneration as a way to circumvent slow seed maturation. Sixty days after pollination (DAP), zygotic embryos were isolated from immature fruits and transferred to Murashige & Skoog (MS) medium without plant growth regulator (PGR) or with different concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ), indole-3-acetic acid (IAA) and a combination of TDZ and IAA. After 147 days, survival ranged from 2 to 44%. Embryos on PGR-free media developed thick fleshy shoots. All treatments developed callus to varying degrees and TDZ induced somatic embryos that subsequently developed into shoot clusters. Microshoots also developed from non-embryogenic callus on media containing 1.5 mg/l BAP and directly on media containing IAA. These shoots developed further on hormone-free media and were then rooted on media containing indole-3-butyric acid (IBA). Overall, seedlings were regenerated in 207 days after pollination, compared with the natural seed development period of nearly 390 days, yielding a potential time saving of 47% for progeny generation. This study demonstrates that early embryo regeneration can not only shorten the breeding process, but also rapidly produce many clones so that their value can be tested immediately in different environments.
Keywords
Horticulture, Melia Volkensii, Regeneration, Immature zygotic embryos, Somatic embryogenesis, Breeding, SOMATIC EMBRYOGENESIS, PLANT-REGENERATION, CULTURE, THIDIAZURON, EFFICIENT, ROOT

Downloads

  • Download
    (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.38 MB

Citation

Please use this url to cite or link to this publication:

MLA
Kimani, Priscilla N., et al. “In Vitro Regeneration of Immature Zygotic Embryos of Melia Volkensii Gürke for Accelerated Breeding.” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 156, no. 1, 2024, doi:10.1007/s11240-023-02615-2.
APA
Kimani, P. N., Wamalwa, L. N., Ngugi, K., Omondi, S. F., Machua, J. M., Magomere, T., … Werbrouck, S. (2024). In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding. PLANT CELL TISSUE AND ORGAN CULTURE, 156(1). https://doi.org/10.1007/s11240-023-02615-2
Chicago author-date
Kimani, Priscilla N., Lydia N. Wamalwa, Kahiu Ngugi, Stephen F. Omondi, Joseph M. Machua, Titus Magomere, Jackson Mulatya, and Stefaan Werbrouck. 2024. “In Vitro Regeneration of Immature Zygotic Embryos of Melia Volkensii Gürke for Accelerated Breeding.” PLANT CELL TISSUE AND ORGAN CULTURE 156 (1). https://doi.org/10.1007/s11240-023-02615-2.
Chicago author-date (all authors)
Kimani, Priscilla N., Lydia N. Wamalwa, Kahiu Ngugi, Stephen F. Omondi, Joseph M. Machua, Titus Magomere, Jackson Mulatya, and Stefaan Werbrouck. 2024. “In Vitro Regeneration of Immature Zygotic Embryos of Melia Volkensii Gürke for Accelerated Breeding.” PLANT CELL TISSUE AND ORGAN CULTURE 156 (1). doi:10.1007/s11240-023-02615-2.
Vancouver
1.
Kimani PN, Wamalwa LN, Ngugi K, Omondi SF, Machua JM, Magomere T, et al. In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding. PLANT CELL TISSUE AND ORGAN CULTURE. 2024;156(1).
IEEE
[1]
P. N. Kimani et al., “In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding,” PLANT CELL TISSUE AND ORGAN CULTURE, vol. 156, no. 1, 2024.
@article{01HHV6EMHXNYW98QPCA130ADKJ,
  abstract     = {{Melia volkensii is a dryland mahogany tree species in East Africa that can be harvested for timber in 15 years and has potential for commercial planting in arid and semi-arid areas. The current breeding programme focusing on growth rate, growth habit and shape is delayed by the long juvenile period and slow seed development. This study evaluated early embryo regeneration as a way to circumvent slow seed maturation. Sixty days after pollination (DAP), zygotic embryos were isolated from immature fruits and transferred to Murashige & Skoog (MS) medium without plant growth regulator (PGR) or with different concentrations of 6-benzylaminopurine (BAP), thidiazuron (TDZ), indole-3-acetic acid (IAA) and a combination of TDZ and IAA. After 147 days, survival ranged from 2 to 44%. Embryos on PGR-free media developed thick fleshy shoots. All treatments developed callus to varying degrees and TDZ induced somatic embryos that subsequently developed into shoot clusters. Microshoots also developed from non-embryogenic callus on media containing 1.5 mg/l BAP and directly on media containing IAA. These shoots developed further on hormone-free media and were then rooted on media containing indole-3-butyric acid (IBA). Overall, seedlings were regenerated in 207 days after pollination, compared with the natural seed development period of nearly 390 days, yielding a potential time saving of 47% for progeny generation. This study demonstrates that early embryo regeneration can not only shorten the breeding process, but also rapidly produce many clones so that their value can be tested immediately in different environments.}},
  articleno    = {{27}},
  author       = {{Kimani, Priscilla N. and Wamalwa, Lydia N. and Ngugi, Kahiu and Omondi, Stephen F. and Machua, Joseph M. and Magomere, Titus and Mulatya, Jackson and Werbrouck, Stefaan}},
  issn         = {{0167-6857}},
  journal      = {{PLANT CELL TISSUE AND ORGAN CULTURE}},
  keywords     = {{Horticulture,Melia Volkensii,Regeneration,Immature zygotic embryos,Somatic embryogenesis,Breeding,SOMATIC EMBRYOGENESIS,PLANT-REGENERATION,CULTURE,THIDIAZURON,EFFICIENT,ROOT}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{9}},
  title        = {{In vitro regeneration of immature zygotic embryos of Melia Volkensii Gürke for accelerated breeding}},
  url          = {{http://doi.org/10.1007/s11240-023-02615-2}},
  volume       = {{156}},
  year         = {{2024}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: