Lost in space? Unmasking the T cell reaction to simulated space stressors
- Author
- Silvana Ferreira Da Silva Miranda (UGent) , Randy Vermeesen, Wilhelmina Radstake, Alessio Parisi, Anna Ivanova, Sarah Baatout (UGent) , Kevin Tabury and Bjorn Baselet
- Organization
- Abstract
- The space environment will expose astronauts to stressors like ionizing radiation, altered gravity fields and elevated cortisol levels, which pose a health risk. Understanding how the interplay between these stressors changes T cells’ response is important to better characterize space-related immune dysfunction. We have exposed stimulated Jurkat cells to simulated space stressors (1 Gy, carbon ions/1 Gy photons, 1 µM hydrocortisone (HC), Mars, moon, and microgravity) in a single or combined manner. Pro-inflammatory cytokine IL-2 was measured in the supernatant of Jurkat cells and at the mRNA level. Results show that alone, HC, Mars gravity and microgravity significantly decrease IL-2 presence in the supernatant. 1 Gy carbon ion irradiation showed a smaller impact on IL-2 levels than photon irradiation. Combining exposure to different simulated space stressors seems to have less immunosuppressive effects. Gene expression was less impacted at the time-point collected. These findings showcase a complex T cell response to different conditions and suggest the importance of elevated cortisol levels in the context of space flight, also highlighting the need to use simulated partial gravity technologies to better understand the immune system’s response to the space environment.
- Keywords
- Inorganic Chemistry, Organic Chemistry, Physical and Theoretical Chemistry, Computer Science Applications, Spectroscopy, Molecular Biology, General Medicine, Catalysis, altered gravity, space radiobiology, stress immunity
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HHT34RB4YQ128W4TM62YBZYK
- MLA
- Ferreira Da Silva Miranda, Silvana, et al. “Lost in Space? Unmasking the T Cell Reaction to Simulated Space Stressors.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 23, MDPI AG, 2023, doi:10.3390/ijms242316943.
- APA
- Ferreira Da Silva Miranda, S., Vermeesen, R., Radstake, W., Parisi, A., Ivanova, A., Baatout, S., … Baselet, B. (2023). Lost in space? Unmasking the T cell reaction to simulated space stressors. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(23). https://doi.org/10.3390/ijms242316943
- Chicago author-date
- Ferreira Da Silva Miranda, Silvana, Randy Vermeesen, Wilhelmina Radstake, Alessio Parisi, Anna Ivanova, Sarah Baatout, Kevin Tabury, and Bjorn Baselet. 2023. “Lost in Space? Unmasking the T Cell Reaction to Simulated Space Stressors.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (23). https://doi.org/10.3390/ijms242316943.
- Chicago author-date (all authors)
- Ferreira Da Silva Miranda, Silvana, Randy Vermeesen, Wilhelmina Radstake, Alessio Parisi, Anna Ivanova, Sarah Baatout, Kevin Tabury, and Bjorn Baselet. 2023. “Lost in Space? Unmasking the T Cell Reaction to Simulated Space Stressors.” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 (23). doi:10.3390/ijms242316943.
- Vancouver
- 1.Ferreira Da Silva Miranda S, Vermeesen R, Radstake W, Parisi A, Ivanova A, Baatout S, et al. Lost in space? Unmasking the T cell reaction to simulated space stressors. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. 2023;24(23).
- IEEE
- [1]S. Ferreira Da Silva Miranda et al., “Lost in space? Unmasking the T cell reaction to simulated space stressors,” INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, vol. 24, no. 23, 2023.
@article{01HHT34RB4YQ128W4TM62YBZYK,
abstract = {{The space environment will expose astronauts to stressors like ionizing radiation, altered gravity fields and elevated cortisol levels, which pose a health risk. Understanding how the interplay between these stressors changes T cells’ response is important to better characterize space-related immune dysfunction. We have exposed stimulated Jurkat cells to simulated space stressors (1 Gy, carbon ions/1 Gy photons, 1 µM hydrocortisone (HC), Mars, moon, and microgravity) in a single or combined manner. Pro-inflammatory cytokine IL-2 was measured in the supernatant of Jurkat cells and at the mRNA level. Results show that alone, HC, Mars gravity and microgravity significantly decrease IL-2 presence in the supernatant. 1 Gy carbon ion irradiation showed a smaller impact on IL-2 levels than photon irradiation. Combining exposure to different simulated space stressors seems to have less immunosuppressive effects. Gene expression was less impacted at the time-point collected. These findings showcase a complex T cell response to different conditions and suggest the importance of elevated cortisol levels in the context of space flight, also highlighting the need to use simulated partial gravity technologies to better understand the immune system’s response to the space environment.}},
articleno = {{16943}},
author = {{Ferreira Da Silva Miranda, Silvana and Vermeesen, Randy and Radstake, Wilhelmina and Parisi, Alessio and Ivanova, Anna and Baatout, Sarah and Tabury, Kevin and Baselet, Bjorn}},
issn = {{1661-6596}},
journal = {{INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES}},
keywords = {{Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis,altered gravity,space radiobiology,stress immunity}},
language = {{eng}},
number = {{23}},
pages = {{21}},
publisher = {{MDPI AG}},
title = {{Lost in space? Unmasking the T cell reaction to simulated space stressors}},
url = {{http://doi.org/10.3390/ijms242316943}},
volume = {{24}},
year = {{2023}},
}
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