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A new plant-parasitic nematode species associated with coffee, Rotylenchus bunae n. sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia

(2023) NEMATOLOGY. 25(7). p.791-800
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Abstract
Rotylenchus bunae n. sp. was discovered in the coffee rhizosphere in Gera district of Jimma, Ethiopia, and has been morphologically and molecularly characterised. The new species is identified by a female body length of 725-876 & mu;m, hemispherical lip region with 4-5 lip annuli, stylet length of 28-30 & mu;m, lateral field areolation only in the pharyngeal region, no cuticular striations, pharyngeal gland overlapping intestine dorsally by 15-26 & mu;m, double epiptygma, no clear spermathecae, vulva position at 54-58% of body length from anterior end, prominent fasciculi in mid and posterior body, rounded or sometimes slightly truncated, coarsely crenated tail with 11-13 annuli, and phasmids located at 7-16 annuli anterior to anus. No males were detected. This species was found closely related to other African Rotylenchus species, such as R. unisexus and R. wimbii; however, it could be well separated from them by both morphology and molecular data (D2-D3 of 28S and partial 18S of rDNA).
Keywords
MOLECULAR PHYLOGENY, MORPHOLOGICAL CHARACTERIZATION, COFFEE, TYLENCHIDA, IRAN, Gera, morphology, morphometrics, nematodes, new species, spiral nematodes, systematics, taxonomy

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MLA
Singh, Phougeishangbam Rolish, et al. “A New Plant-Parasitic Nematode Species Associated with Coffee, Rotylenchus Bunae n. Sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia.” NEMATOLOGY, vol. 25, no. 7, 2023, pp. 791–800, doi:10.1163/15685411-bja10256.
APA
Singh, P. R., Aseffa, A. W., Hajihassani, A., Karssen, G., Bert, W., & Meressa, B. H. (2023). A new plant-parasitic nematode species associated with coffee, Rotylenchus bunae n. sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia. NEMATOLOGY, 25(7), 791–800. https://doi.org/10.1163/15685411-bja10256
Chicago author-date
Singh, Phougeishangbam Rolish, Abebe Woldesenbet Aseffa, Abolfazl Hajihassani, Gerrit Karssen, Wim Bert, and Beira Hailu Meressa. 2023. “A New Plant-Parasitic Nematode Species Associated with Coffee, Rotylenchus Bunae n. Sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia.” NEMATOLOGY 25 (7): 791–800. https://doi.org/10.1163/15685411-bja10256.
Chicago author-date (all authors)
Singh, Phougeishangbam Rolish, Abebe Woldesenbet Aseffa, Abolfazl Hajihassani, Gerrit Karssen, Wim Bert, and Beira Hailu Meressa. 2023. “A New Plant-Parasitic Nematode Species Associated with Coffee, Rotylenchus Bunae n. Sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia.” NEMATOLOGY 25 (7): 791–800. doi:10.1163/15685411-bja10256.
Vancouver
1.
Singh PR, Aseffa AW, Hajihassani A, Karssen G, Bert W, Meressa BH. A new plant-parasitic nematode species associated with coffee, Rotylenchus bunae n. sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia. NEMATOLOGY. 2023;25(7):791–800.
IEEE
[1]
P. R. Singh, A. W. Aseffa, A. Hajihassani, G. Karssen, W. Bert, and B. H. Meressa, “A new plant-parasitic nematode species associated with coffee, Rotylenchus bunae n. sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia,” NEMATOLOGY, vol. 25, no. 7, pp. 791–800, 2023.
@article{01HGDJPF25ED1ZK3E98P0D0BHK,
  abstract     = {{Rotylenchus bunae n. sp. was discovered in the coffee rhizosphere in Gera district of Jimma, Ethiopia, and has been morphologically and molecularly characterised. The new species is identified by a female body length of 725-876 & mu;m, hemispherical lip region with 4-5 lip annuli, stylet length of 28-30 & mu;m, lateral field areolation only in the pharyngeal region, no cuticular striations, pharyngeal gland overlapping intestine dorsally by 15-26 & mu;m, double epiptygma, no clear spermathecae, vulva position at 54-58% of body length from anterior end, prominent fasciculi in mid and posterior body, rounded or sometimes slightly truncated, coarsely crenated tail with 11-13 annuli, and phasmids located at 7-16 annuli anterior to anus. No males were detected. This species was found closely related to other African Rotylenchus species, such as R. unisexus and R. wimbii; however, it could be well separated from them by both morphology and molecular data (D2-D3 of 28S and partial 18S of rDNA).}},
  author       = {{Singh, Phougeishangbam Rolish and Aseffa, Abebe Woldesenbet and  Hajihassani, Abolfazl and Karssen, Gerrit and Bert, Wim and  Meressa, Beira Hailu}},
  issn         = {{1388-5545}},
  journal      = {{NEMATOLOGY}},
  keywords     = {{MOLECULAR PHYLOGENY,MORPHOLOGICAL CHARACTERIZATION,COFFEE,TYLENCHIDA,IRAN,Gera,morphology,morphometrics,nematodes,new species,spiral nematodes,systematics,taxonomy}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{791--800}},
  title        = {{A new plant-parasitic nematode species associated with coffee, Rotylenchus bunae n. sp. (Nematoda: Hoplolaimidae) from Jimma, Ethiopia}},
  url          = {{http://doi.org/10.1163/15685411-bja10256}},
  volume       = {{25}},
  year         = {{2023}},
}

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