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In vitro investigation into the effect of cryopreservation on the mechanical characteristics of dental hard tissues

Noëmi De Roo (UGent) , Kaat Toulouse (UGent) , Laurent Thierens (UGent) , Silke Henry, Stefanie De Buyser (UGent) , Liesbeth Temmerman (UGent) , Ronald Verbeeck (UGent) and Guy De Pauw (UGent)
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Abstract
Previous research has reported on hidden damage within the dentin introduced by cryopreservation, but the effect on the mechanical properties of the hard tissues at tooth level remains unclear. The main objective of this study is to investigate the effect of cryopreservation on the mechanical properties of teeth. A matched sample of 234 premolars of 117 children (9 ≤ age ≤ 16 years), bilaterally extracted for orthodontic reasons, were included. For each child, one tooth was randomly allocated to the cryopreservation group and the contralateral tooth was assigned to the control group. Static compression tests were performed to determine load to failure, stiffness, and toughness. In a subgroup of 20 teeth, a cyclic preloading or chewing simulation was performed. Additionally, the fracture mode was determined, and the microstructure of the fractured surfaces was examined using a scanning electron microscope (SEM). Linear mixed model analyses could not detect a statistical difference in the mean load to failure (p = 0.549), mean toughness (p = 0.968), or mean stiffness (p = 0.150) between cryopreserved and non-cryopreserved teeth. No significant difference in load to failure after cyclic preloading was detected between groups (p = 0.734). SEM analysis revealed comparable fracture characteristics between groups. It is concluded that cryopreservation does not affect the mean load to failure, stiffness, or toughness of teeth, indicating that hidden damage in the dentin is not critical at tooth level.
Keywords
Biomedical Engineering, Biomaterials, scanning electron microscope, fracture, mechanical properties, cryopreservation, teeth

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MLA
De Roo, Noëmi, et al. “In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues.” JOURNAL OF FUNCTIONAL BIOMATERIALS, vol. 14, no. 11, MDPI AG, 2023, doi:10.3390/jfb14110551.
APA
De Roo, N., Toulouse, K., Thierens, L., Henry, S., De Buyser, S., Temmerman, L., … De Pauw, G. (2023). In vitro investigation into the effect of cryopreservation on the mechanical characteristics of dental hard tissues. JOURNAL OF FUNCTIONAL BIOMATERIALS, 14(11). https://doi.org/10.3390/jfb14110551
Chicago author-date
De Roo, Noëmi, Kaat Toulouse, Laurent Thierens, Silke Henry, Stefanie De Buyser, Liesbeth Temmerman, Ronald Verbeeck, and Guy De Pauw. 2023. “In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues.” JOURNAL OF FUNCTIONAL BIOMATERIALS 14 (11). https://doi.org/10.3390/jfb14110551.
Chicago author-date (all authors)
De Roo, Noëmi, Kaat Toulouse, Laurent Thierens, Silke Henry, Stefanie De Buyser, Liesbeth Temmerman, Ronald Verbeeck, and Guy De Pauw. 2023. “In Vitro Investigation into the Effect of Cryopreservation on the Mechanical Characteristics of Dental Hard Tissues.” JOURNAL OF FUNCTIONAL BIOMATERIALS 14 (11). doi:10.3390/jfb14110551.
Vancouver
1.
De Roo N, Toulouse K, Thierens L, Henry S, De Buyser S, Temmerman L, et al. In vitro investigation into the effect of cryopreservation on the mechanical characteristics of dental hard tissues. JOURNAL OF FUNCTIONAL BIOMATERIALS. 2023;14(11).
IEEE
[1]
N. De Roo et al., “In vitro investigation into the effect of cryopreservation on the mechanical characteristics of dental hard tissues,” JOURNAL OF FUNCTIONAL BIOMATERIALS, vol. 14, no. 11, 2023.
@article{01HG7YQ03E4AWN66NFWSGVZ7Q1,
  abstract     = {{Previous research has reported on hidden damage within the dentin introduced by cryopreservation, but the effect on the mechanical properties of the hard tissues at tooth level remains unclear. The main objective of this study is to investigate the effect of cryopreservation on the mechanical properties of teeth. A matched sample of 234 premolars of 117 children (9 ≤ age ≤ 16 years), bilaterally extracted for orthodontic reasons, were included. For each child, one tooth was randomly allocated to the cryopreservation group and the contralateral tooth was assigned to the control group. Static compression tests were performed to determine load to failure, stiffness, and toughness. In a subgroup of 20 teeth, a cyclic preloading or chewing simulation was performed. Additionally, the fracture mode was determined, and the microstructure of the fractured surfaces was examined using a scanning electron microscope (SEM). Linear mixed model analyses could not detect a statistical difference in the mean load to failure (p = 0.549), mean toughness (p = 0.968), or mean stiffness (p = 0.150) between cryopreserved and non-cryopreserved teeth. No significant difference in load to failure after cyclic preloading was detected between groups (p = 0.734). SEM analysis revealed comparable fracture characteristics between groups. It is concluded that cryopreservation does not affect the mean load to failure, stiffness, or toughness of teeth, indicating that hidden damage in the dentin is not critical at tooth level.}},
  articleno    = {{551}},
  author       = {{De Roo, Noëmi and Toulouse, Kaat and Thierens, Laurent and Henry, Silke and De Buyser, Stefanie and Temmerman, Liesbeth and Verbeeck, Ronald and De Pauw, Guy}},
  issn         = {{2079-4983}},
  journal      = {{JOURNAL OF FUNCTIONAL BIOMATERIALS}},
  keywords     = {{Biomedical Engineering,Biomaterials,scanning electron microscope,fracture,mechanical properties,cryopreservation,teeth}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{12}},
  publisher    = {{MDPI AG}},
  title        = {{In vitro investigation into the effect of cryopreservation on the mechanical characteristics of dental hard tissues}},
  url          = {{http://doi.org/10.3390/jfb14110551}},
  volume       = {{14}},
  year         = {{2023}},
}

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