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New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications

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Abstract
Surface modification of hydrophobic nanofibers (NFs) to introduce cell-interactive chemical functionalities re-mains a challenge in biomedical applications. This study presents a novel three-step plasma-based method for synthesizing coatings with improved chemical selectivity compared to conventional plasma polymers. The process involved hexamethyldisiloxane (HMDSO) plasma polymerization followed by helium plasma activation, both performed in a medium-pressure dielectric barrier discharge. Scanning electron microscopy analysis demonstrated that the plasma-based steps did not cause damage to the NFs. X-ray photoelectron spectroscopy (XPS) and water contact angle measurements revealed the formation of a hydrophilic silanol-rich layer after HMDSO plasma polymerization and helium plasma activation. In the third step, (3-aminopropyl)triethoxysilane (APTES) was grafted onto the plasma polymer to introduce primary amine groups onto the surface, as confirmed by XPS. Although the APTES-based layer exhibited partial removal when exposed to aqueous environments, a stable aminated layer remained on the NF surface, which significantly enhanced Schwann cell responses compared to untreated and HMDSO-based coated NFs. This enhancement was confirmed through fluorescent imaging using live-dead staining, immunostaining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazo-lium bromide (MTT) assay. These coatings with a high selectivity in their chemical functionality (amines, or other functionalities via silanization agent selection), offer a promising surface functionalization approach for tissue engineering scaffolds.
Keywords
Surfaces, Coatings and Films, Condensed Matter Physics, Surfaces and Interfaces, General Physics and Astronomy, General Chemistry, Tissue engineering, Electrospinning, HMDSO plasma polymerization, Plasma, activation, Primary amines, Schwann cells, SURFACE-CHEMISTRY, POLYMERIC NANOFIBERS, PCL NANOFIBERS, SCHWANN-CELLS, STEM-CELLS, ADHESION, FILMS, HEXAMETHYLDISILOXANE, FUNCTIONALIZATION, DIFFERENTIATION

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MLA
Aliakbarshirazi, Sheida, et al. “New Plasma-Assisted Polymerization/Activation Route Leading to a High Density Primary Amine Silanization of PCL/PLGA Nanofibers for Biomedical Applications.” APPLIED SURFACE SCIENCE, vol. 640, 2023, doi:10.1016/j.apsusc.2023.158380.
APA
Aliakbarshirazi, S., Ghobeira, R., Egghe, T., De Geyter, N., Declercq, H., & Morent, R. (2023). New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications. APPLIED SURFACE SCIENCE, 640. https://doi.org/10.1016/j.apsusc.2023.158380
Chicago author-date
Aliakbarshirazi, Sheida, Rouba Ghobeira, Tim Egghe, Nathalie De Geyter, Heidi Declercq, and Rino Morent. 2023. “New Plasma-Assisted Polymerization/Activation Route Leading to a High Density Primary Amine Silanization of PCL/PLGA Nanofibers for Biomedical Applications.” APPLIED SURFACE SCIENCE 640. https://doi.org/10.1016/j.apsusc.2023.158380.
Chicago author-date (all authors)
Aliakbarshirazi, Sheida, Rouba Ghobeira, Tim Egghe, Nathalie De Geyter, Heidi Declercq, and Rino Morent. 2023. “New Plasma-Assisted Polymerization/Activation Route Leading to a High Density Primary Amine Silanization of PCL/PLGA Nanofibers for Biomedical Applications.” APPLIED SURFACE SCIENCE 640. doi:10.1016/j.apsusc.2023.158380.
Vancouver
1.
Aliakbarshirazi S, Ghobeira R, Egghe T, De Geyter N, Declercq H, Morent R. New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications. APPLIED SURFACE SCIENCE. 2023;640.
IEEE
[1]
S. Aliakbarshirazi, R. Ghobeira, T. Egghe, N. De Geyter, H. Declercq, and R. Morent, “New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications,” APPLIED SURFACE SCIENCE, vol. 640, 2023.
@article{01HEQRTD79JSQ4SQBR121TVQGK,
  abstract     = {{Surface modification of hydrophobic nanofibers (NFs) to introduce cell-interactive chemical functionalities re-mains a challenge in biomedical applications. This study presents a novel three-step plasma-based method for synthesizing coatings with improved chemical selectivity compared to conventional plasma polymers. The process involved hexamethyldisiloxane (HMDSO) plasma polymerization followed by helium plasma activation, both performed in a medium-pressure dielectric barrier discharge. Scanning electron microscopy analysis demonstrated that the plasma-based steps did not cause damage to the NFs. X-ray photoelectron spectroscopy (XPS) and water contact angle measurements revealed the formation of a hydrophilic silanol-rich layer after HMDSO plasma polymerization and helium plasma activation. In the third step, (3-aminopropyl)triethoxysilane (APTES) was grafted onto the plasma polymer to introduce primary amine groups onto the surface, as confirmed by XPS. Although the APTES-based layer exhibited partial removal when exposed to aqueous environments, a stable aminated layer remained on the NF surface, which significantly enhanced Schwann cell responses compared to untreated and HMDSO-based coated NFs. This enhancement was confirmed through fluorescent imaging using live-dead staining, immunostaining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazo-lium bromide (MTT) assay. These coatings with a high selectivity in their chemical functionality (amines, or other functionalities via silanization agent selection), offer a promising surface functionalization approach for tissue engineering scaffolds.}},
  articleno    = {{158380}},
  author       = {{Aliakbarshirazi, Sheida and Ghobeira, Rouba and Egghe, Tim and De Geyter, Nathalie and Declercq, Heidi and Morent, Rino}},
  issn         = {{0169-4332}},
  journal      = {{APPLIED SURFACE SCIENCE}},
  keywords     = {{Surfaces, Coatings and Films,Condensed Matter Physics,Surfaces and Interfaces,General Physics and Astronomy,General Chemistry,Tissue engineering,Electrospinning,HMDSO plasma polymerization,Plasma,activation,Primary amines,Schwann cells,SURFACE-CHEMISTRY,POLYMERIC NANOFIBERS,PCL NANOFIBERS,SCHWANN-CELLS,STEM-CELLS,ADHESION,FILMS,HEXAMETHYLDISILOXANE,FUNCTIONALIZATION,DIFFERENTIATION}},
  language     = {{eng}},
  pages        = {{20}},
  title        = {{New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications}},
  url          = {{http://doi.org/10.1016/j.apsusc.2023.158380}},
  volume       = {{640}},
  year         = {{2023}},
}

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