
New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications
- Author
- Sheida Aliakbarshirazi (UGent) , Rouba Ghobeira (UGent) , Tim Egghe, Nathalie De Geyter (UGent) , Heidi Declercq and Rino Morent (UGent)
- Organization
- Abstract
- Surface modification of hydrophobic nanofibers (NFs) to introduce cell-interactive chemical functionalities re-mains a challenge in biomedical applications. This study presents a novel three-step plasma-based method for synthesizing coatings with improved chemical selectivity compared to conventional plasma polymers. The process involved hexamethyldisiloxane (HMDSO) plasma polymerization followed by helium plasma activation, both performed in a medium-pressure dielectric barrier discharge. Scanning electron microscopy analysis demonstrated that the plasma-based steps did not cause damage to the NFs. X-ray photoelectron spectroscopy (XPS) and water contact angle measurements revealed the formation of a hydrophilic silanol-rich layer after HMDSO plasma polymerization and helium plasma activation. In the third step, (3-aminopropyl)triethoxysilane (APTES) was grafted onto the plasma polymer to introduce primary amine groups onto the surface, as confirmed by XPS. Although the APTES-based layer exhibited partial removal when exposed to aqueous environments, a stable aminated layer remained on the NF surface, which significantly enhanced Schwann cell responses compared to untreated and HMDSO-based coated NFs. This enhancement was confirmed through fluorescent imaging using live-dead staining, immunostaining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazo-lium bromide (MTT) assay. These coatings with a high selectivity in their chemical functionality (amines, or other functionalities via silanization agent selection), offer a promising surface functionalization approach for tissue engineering scaffolds.
- Keywords
- Surfaces, Coatings and Films, Condensed Matter Physics, Surfaces and Interfaces, General Physics and Astronomy, General Chemistry, Tissue engineering, Electrospinning, HMDSO plasma polymerization, Plasma, activation, Primary amines, Schwann cells, SURFACE-CHEMISTRY, POLYMERIC NANOFIBERS, PCL NANOFIBERS, SCHWANN-CELLS, STEM-CELLS, ADHESION, FILMS, HEXAMETHYLDISILOXANE, FUNCTIONALIZATION, DIFFERENTIATION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HEQRTD79JSQ4SQBR121TVQGK
- MLA
- Aliakbarshirazi, Sheida, et al. “New Plasma-Assisted Polymerization/Activation Route Leading to a High Density Primary Amine Silanization of PCL/PLGA Nanofibers for Biomedical Applications.” APPLIED SURFACE SCIENCE, vol. 640, 2023, doi:10.1016/j.apsusc.2023.158380.
- APA
- Aliakbarshirazi, S., Ghobeira, R., Egghe, T., De Geyter, N., Declercq, H., & Morent, R. (2023). New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications. APPLIED SURFACE SCIENCE, 640. https://doi.org/10.1016/j.apsusc.2023.158380
- Chicago author-date
- Aliakbarshirazi, Sheida, Rouba Ghobeira, Tim Egghe, Nathalie De Geyter, Heidi Declercq, and Rino Morent. 2023. “New Plasma-Assisted Polymerization/Activation Route Leading to a High Density Primary Amine Silanization of PCL/PLGA Nanofibers for Biomedical Applications.” APPLIED SURFACE SCIENCE 640. https://doi.org/10.1016/j.apsusc.2023.158380.
- Chicago author-date (all authors)
- Aliakbarshirazi, Sheida, Rouba Ghobeira, Tim Egghe, Nathalie De Geyter, Heidi Declercq, and Rino Morent. 2023. “New Plasma-Assisted Polymerization/Activation Route Leading to a High Density Primary Amine Silanization of PCL/PLGA Nanofibers for Biomedical Applications.” APPLIED SURFACE SCIENCE 640. doi:10.1016/j.apsusc.2023.158380.
- Vancouver
- 1.Aliakbarshirazi S, Ghobeira R, Egghe T, De Geyter N, Declercq H, Morent R. New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications. APPLIED SURFACE SCIENCE. 2023;640.
- IEEE
- [1]S. Aliakbarshirazi, R. Ghobeira, T. Egghe, N. De Geyter, H. Declercq, and R. Morent, “New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications,” APPLIED SURFACE SCIENCE, vol. 640, 2023.
@article{01HEQRTD79JSQ4SQBR121TVQGK, abstract = {{Surface modification of hydrophobic nanofibers (NFs) to introduce cell-interactive chemical functionalities re-mains a challenge in biomedical applications. This study presents a novel three-step plasma-based method for synthesizing coatings with improved chemical selectivity compared to conventional plasma polymers. The process involved hexamethyldisiloxane (HMDSO) plasma polymerization followed by helium plasma activation, both performed in a medium-pressure dielectric barrier discharge. Scanning electron microscopy analysis demonstrated that the plasma-based steps did not cause damage to the NFs. X-ray photoelectron spectroscopy (XPS) and water contact angle measurements revealed the formation of a hydrophilic silanol-rich layer after HMDSO plasma polymerization and helium plasma activation. In the third step, (3-aminopropyl)triethoxysilane (APTES) was grafted onto the plasma polymer to introduce primary amine groups onto the surface, as confirmed by XPS. Although the APTES-based layer exhibited partial removal when exposed to aqueous environments, a stable aminated layer remained on the NF surface, which significantly enhanced Schwann cell responses compared to untreated and HMDSO-based coated NFs. This enhancement was confirmed through fluorescent imaging using live-dead staining, immunostaining, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazo-lium bromide (MTT) assay. These coatings with a high selectivity in their chemical functionality (amines, or other functionalities via silanization agent selection), offer a promising surface functionalization approach for tissue engineering scaffolds.}}, articleno = {{158380}}, author = {{Aliakbarshirazi, Sheida and Ghobeira, Rouba and Egghe, Tim and De Geyter, Nathalie and Declercq, Heidi and Morent, Rino}}, issn = {{0169-4332}}, journal = {{APPLIED SURFACE SCIENCE}}, keywords = {{Surfaces, Coatings and Films,Condensed Matter Physics,Surfaces and Interfaces,General Physics and Astronomy,General Chemistry,Tissue engineering,Electrospinning,HMDSO plasma polymerization,Plasma,activation,Primary amines,Schwann cells,SURFACE-CHEMISTRY,POLYMERIC NANOFIBERS,PCL NANOFIBERS,SCHWANN-CELLS,STEM-CELLS,ADHESION,FILMS,HEXAMETHYLDISILOXANE,FUNCTIONALIZATION,DIFFERENTIATION}}, language = {{eng}}, pages = {{20}}, title = {{New plasma-assisted polymerization/activation route leading to a high density primary amine silanization of PCL/PLGA nanofibers for biomedical applications}}, url = {{http://doi.org/10.1016/j.apsusc.2023.158380}}, volume = {{640}}, year = {{2023}}, }
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