
The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing
- Author
- Susana Endah Ratnawati (UGent) , Lotta Kuuliala (UGent) , Nele Verschuere (UGent) , Margo Cnockaert (UGent) , Peter Vandamme (UGent) and Frank Devlieghere (UGent)
- Organization
- Project
- Abstract
- Few studies have addressed species-level identification of spoilage bacteria in blue mussels packed under modified atmospheres (MAs). We investigated the effect of MAs and seasons on the tentative species-level of dominant spoilage bacteria in blue mussels. Summer (s) and winter (w) blue mussels were stored at 4 °C in the atmospheres (%CO2/O2/N2): A40s (30/40/30), B60s (40/60/0), C60s (0/60/40), A40w (30/40/30), and D75w (25/75/0). In total, 122 culturable isolates were obtained at the final stage of shelf life, when mortality was high (56–100%) and total psychrotrophic bacteria counted > 7 log CFU g-1. Biochemical properties were analyzed using gram reactions, catalase and oxidase activities, and salt tolerance tests. Culturable isolates were identified through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequence analysis. Spoilage potential tests were investigated by evaluating protease, lipase, and fermentation activities as well as gas and H2S production. The culturable isolates showed tolerance to varied salt concentrations. Psychromonas arctica, Pseudoalteromonas elyakovii, and Shewanella frigidimarina were dominating in specific MAs. Winter blue mussels resulted in a higher variation of spoilage bacteria, including S. frigidimarina, S. vesiculosa, S. polaris, Micrococcus luteus, Paeniglutamicibacter terrestris sp. nov., and Alteromonas sp.
- Keywords
- Microbiology, Food Science, Spoilage bacteria, 16S rRNA gene sequencing, MALDI-TOF MS, Blue mussels, Atmospheres
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01HDJXKHJ0FDS3JH02PM7TSDP7
- MLA
- Ratnawati, Susana Endah, et al. “The Exploration of Dominant Spoilage Bacteria in Blue Mussels (Mytilus Edulis) Stored under Different Modified Atmospheres by MALDI-TOF MS in Combination with 16S RRNA Sequencing.” FOOD MICROBIOLOGY, vol. 118, Elsevier BV, 2024, doi:10.1016/j.fm.2023.104407.
- APA
- Ratnawati, S. E., Kuuliala, L., Verschuere, N., Cnockaert, M., Vandamme, P., & Devlieghere, F. (2024). The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing. FOOD MICROBIOLOGY, 118. https://doi.org/10.1016/j.fm.2023.104407
- Chicago author-date
- Ratnawati, Susana Endah, Lotta Kuuliala, Nele Verschuere, Margo Cnockaert, Peter Vandamme, and Frank Devlieghere. 2024. “The Exploration of Dominant Spoilage Bacteria in Blue Mussels (Mytilus Edulis) Stored under Different Modified Atmospheres by MALDI-TOF MS in Combination with 16S RRNA Sequencing.” FOOD MICROBIOLOGY 118. https://doi.org/10.1016/j.fm.2023.104407.
- Chicago author-date (all authors)
- Ratnawati, Susana Endah, Lotta Kuuliala, Nele Verschuere, Margo Cnockaert, Peter Vandamme, and Frank Devlieghere. 2024. “The Exploration of Dominant Spoilage Bacteria in Blue Mussels (Mytilus Edulis) Stored under Different Modified Atmospheres by MALDI-TOF MS in Combination with 16S RRNA Sequencing.” FOOD MICROBIOLOGY 118. doi:10.1016/j.fm.2023.104407.
- Vancouver
- 1.Ratnawati SE, Kuuliala L, Verschuere N, Cnockaert M, Vandamme P, Devlieghere F. The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing. FOOD MICROBIOLOGY. 2024;118.
- IEEE
- [1]S. E. Ratnawati, L. Kuuliala, N. Verschuere, M. Cnockaert, P. Vandamme, and F. Devlieghere, “The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing,” FOOD MICROBIOLOGY, vol. 118, 2024.
@article{01HDJXKHJ0FDS3JH02PM7TSDP7, abstract = {{Few studies have addressed species-level identification of spoilage bacteria in blue mussels packed under modified atmospheres (MAs). We investigated the effect of MAs and seasons on the tentative species-level of dominant spoilage bacteria in blue mussels. Summer (s) and winter (w) blue mussels were stored at 4 °C in the atmospheres (%CO2/O2/N2): A40s (30/40/30), B60s (40/60/0), C60s (0/60/40), A40w (30/40/30), and D75w (25/75/0). In total, 122 culturable isolates were obtained at the final stage of shelf life, when mortality was high (56–100%) and total psychrotrophic bacteria counted > 7 log CFU g-1. Biochemical properties were analyzed using gram reactions, catalase and oxidase activities, and salt tolerance tests. Culturable isolates were identified through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequence analysis. Spoilage potential tests were investigated by evaluating protease, lipase, and fermentation activities as well as gas and H2S production. The culturable isolates showed tolerance to varied salt concentrations. Psychromonas arctica, Pseudoalteromonas elyakovii, and Shewanella frigidimarina were dominating in specific MAs. Winter blue mussels resulted in a higher variation of spoilage bacteria, including S. frigidimarina, S. vesiculosa, S. polaris, Micrococcus luteus, Paeniglutamicibacter terrestris sp. nov., and Alteromonas sp. }}, articleno = {{104407}}, author = {{Ratnawati, Susana Endah and Kuuliala, Lotta and Verschuere, Nele and Cnockaert, Margo and Vandamme, Peter and Devlieghere, Frank}}, issn = {{0740-0020}}, journal = {{FOOD MICROBIOLOGY}}, keywords = {{Microbiology,Food Science,Spoilage bacteria,16S rRNA gene sequencing,MALDI-TOF MS,Blue mussels,Atmospheres}}, language = {{eng}}, pages = {{11}}, publisher = {{Elsevier BV}}, title = {{The exploration of dominant spoilage bacteria in blue mussels (Mytilus edulis) stored under different modified atmospheres by MALDI-TOF MS in combination with 16S rRNA sequencing}}, url = {{http://doi.org/10.1016/j.fm.2023.104407}}, volume = {{118}}, year = {{2024}}, }
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