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Liver transcriptomic and methylomic analyses identify transcriptional mitogen-activated protein kinase regulation in facultative hibernation of Syrian hamster

Louis Coussement (UGent) , Marloes M. Oosterhof, Victor Guryev, Vera A. Reitsema, Jojanneke J. Bruintjes, Maaike Goris, Hjalmar R. Bouma, Tim De Meyer (UGent) , Marianne G. Rots and Robert H. Henning
(2023) PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES. 290(1999).
Author
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Abstract
Hibernation consists of alternating torpor-arousal phases, during which animals cope with repetitive hypothermia and ischaemia-reperfusion. Due to limited transcriptomic and methylomic information for facultative hibernators, we here conducted RNA and whole-genome bisulfide sequencing in liver of hibernating Syrian hamster (Mesocricetus auratus). Gene ontology analysis was performed on 844 differentially expressed genes and confirmed the shift in metabolic fuel utilization, inhibition of RNA transcription and cell cycle regulation as found in seasonal hibernators. Additionally, we showed a so far unreported suppression of mitogen-activated protein kinase (MAPK) and protein phosphatase 1 pathways during torpor. Notably, hibernating hamsters showed upregulation of MAPK inhibitors (dual-specificity phosphatases and sproutys) and reduced levels of MAPK-induced transcription factors (TFs). Promoter methylation was found to modulate the expression of genes targeted by these TFs. In conclusion, we document gene regulation between hibernation phases, which may aid the identification of pathways and targets to prevent organ damage in transplantation or ischaemia-reperfusion.
Keywords
facultative hibernator, torpor, gene regulation, cell cycle, transcription factor-binding sites, DNA methylation, GROUND-SQUIRREL, MAMMALIAN HIBERNATION, CELL-PROLIFERATION, DNA METHYLATION, EXPRESSION, CYCLE, PHOSPHORYLATION, SUPPRESSION, METABOLISM, RESISTANCE

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Citation

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MLA
Coussement, Louis, et al. “Liver Transcriptomic and Methylomic Analyses Identify Transcriptional Mitogen-Activated Protein Kinase Regulation in Facultative Hibernation of Syrian Hamster.” PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, vol. 290, no. 1999, 2023, doi:10.1098/rspb.2023.0368.
APA
Coussement, L., Oosterhof, M. M., Guryev, V., Reitsema, V. A., Bruintjes, J. J., Goris, M., … Henning, R. H. (2023). Liver transcriptomic and methylomic analyses identify transcriptional mitogen-activated protein kinase regulation in facultative hibernation of Syrian hamster. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 290(1999). https://doi.org/10.1098/rspb.2023.0368
Chicago author-date
Coussement, Louis, Marloes M. Oosterhof, Victor Guryev, Vera A. Reitsema, Jojanneke J. Bruintjes, Maaike Goris, Hjalmar R. Bouma, Tim De Meyer, Marianne G. Rots, and Robert H. Henning. 2023. “Liver Transcriptomic and Methylomic Analyses Identify Transcriptional Mitogen-Activated Protein Kinase Regulation in Facultative Hibernation of Syrian Hamster.” PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 290 (1999). https://doi.org/10.1098/rspb.2023.0368.
Chicago author-date (all authors)
Coussement, Louis, Marloes M. Oosterhof, Victor Guryev, Vera A. Reitsema, Jojanneke J. Bruintjes, Maaike Goris, Hjalmar R. Bouma, Tim De Meyer, Marianne G. Rots, and Robert H. Henning. 2023. “Liver Transcriptomic and Methylomic Analyses Identify Transcriptional Mitogen-Activated Protein Kinase Regulation in Facultative Hibernation of Syrian Hamster.” PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES 290 (1999). doi:10.1098/rspb.2023.0368.
Vancouver
1.
Coussement L, Oosterhof MM, Guryev V, Reitsema VA, Bruintjes JJ, Goris M, et al. Liver transcriptomic and methylomic analyses identify transcriptional mitogen-activated protein kinase regulation in facultative hibernation of Syrian hamster. PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES. 2023;290(1999).
IEEE
[1]
L. Coussement et al., “Liver transcriptomic and methylomic analyses identify transcriptional mitogen-activated protein kinase regulation in facultative hibernation of Syrian hamster,” PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, vol. 290, no. 1999, 2023.
@article{01HCHE721CP7176C4RJNM6Q94B,
  abstract     = {{Hibernation consists of alternating torpor-arousal phases, during which animals cope with repetitive hypothermia and ischaemia-reperfusion. Due to limited transcriptomic and methylomic information for facultative hibernators, we here conducted RNA and whole-genome bisulfide sequencing in liver of hibernating Syrian hamster (Mesocricetus auratus). Gene ontology analysis was performed on 844 differentially expressed genes and confirmed the shift in metabolic fuel utilization, inhibition of RNA transcription and cell cycle regulation as found in seasonal hibernators. Additionally, we showed a so far unreported suppression of mitogen-activated protein kinase (MAPK) and protein phosphatase 1 pathways during torpor. Notably, hibernating hamsters showed upregulation of MAPK inhibitors (dual-specificity phosphatases and sproutys) and reduced levels of MAPK-induced transcription factors (TFs). Promoter methylation was found to modulate the expression of genes targeted by these TFs. In conclusion, we document gene regulation between hibernation phases, which may aid the identification of pathways and targets to prevent organ damage in transplantation or ischaemia-reperfusion.}},
  articleno    = {{20230368}},
  author       = {{Coussement, Louis and Oosterhof, Marloes M. and  Guryev, Victor and  Reitsema, Vera A. and  Bruintjes, Jojanneke J. and  Goris, Maaike and  Bouma, Hjalmar R. and De Meyer, Tim and Rots, Marianne G. and Henning, Robert H.}},
  issn         = {{0962-8452}},
  journal      = {{PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES}},
  keywords     = {{facultative hibernator,torpor,gene regulation,cell cycle,transcription factor-binding sites,DNA methylation,GROUND-SQUIRREL,MAMMALIAN HIBERNATION,CELL-PROLIFERATION,DNA METHYLATION,EXPRESSION,CYCLE,PHOSPHORYLATION,SUPPRESSION,METABOLISM,RESISTANCE}},
  language     = {{eng}},
  number       = {{1999}},
  pages        = {{11}},
  title        = {{Liver transcriptomic and methylomic analyses identify transcriptional mitogen-activated protein kinase regulation in facultative hibernation of Syrian hamster}},
  url          = {{http://doi.org/10.1098/rspb.2023.0368}},
  volume       = {{290}},
  year         = {{2023}},
}
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