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The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps

Zhaofeng Xu (UGent) , Yanran Huang (UGent) , Tim Meese, Sharon Van Nevel, Gabriële Holtappels (UGent) , Stijn Vanhee (UGent) , Barbara M. Bröker, Zhengqi Li, Ellen De Meester (UGent) , Natalie De Ruyck (UGent) , et al.
(2023) CLINICAL IMMUNOLOGY. 256.
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Abstract
Uncontrolled severe chronic rhinosinusitis with nasal polyps (CRSwNP) is associated with elevated levels of type 2 inflammatory cytokines and raised immunoglobulin concentrations in nasal polyp tissue. By using single-cell RNA sequencing, transcriptomics, surface proteomics, and T cell and B cell receptor sequencing, we found the predominant cell types in nasal polyps were shifted from epithelial and mesenchymal cells to inflammatory cells compared to nasal mucosa from healthy controls. Broad expansions of CD4 T effector memory cells, CD4 tissueresident memory T cells, CD8 T effector memory cells and all subtypes of B cells in nasal polyp tissues. The T and B cell receptor repertoires were skewed in NP. This study highlights the deviated immune response and remodeling mechanisms that contribute to the pathogenesis of uncontrolled severe CRSwNP. Clinical implications: We identified differences in the cellular compositions, transcriptomes, proteomes, and deviations in the immune profiles of T cell and B cell receptors as well as alterations in the intercellular communications in uncontrolled severe CRSwNP patients versus healthy controls, which might help to define potential therapeutic targets in the future.
Keywords
Immunology, Immunology and Allergy, Chronic rhinosinusitis with nasal polyps, scRNA-seq, Immune profiles, Tissue remodeling, Type 2 immune responses, DIFFERENTIAL EXPRESSION ANALYSIS, T-CELLS, SUBSETS, TISSUE, SUBPOPULATION, ACTIVATION, MIGRATION, PACKAGE, IGE

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MLA
Xu, Zhaofeng, et al. “The Multi-Omics Single-Cell Landscape of Sinus Mucosa in Uncontrolled Severe Chronic Rhinosinusitis with Nasal Polyps.” CLINICAL IMMUNOLOGY, vol. 256, 2023, doi:10.1016/j.clim.2023.109791.
APA
Xu, Z., Huang, Y., Meese, T., Van Nevel, S., Holtappels, G., Vanhee, S., … Bachert, C. (2023). The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps. CLINICAL IMMUNOLOGY, 256. https://doi.org/10.1016/j.clim.2023.109791
Chicago author-date
Xu, Zhaofeng, Yanran Huang, Tim Meese, Sharon Van Nevel, Gabriële Holtappels, Stijn Vanhee, Barbara M. Bröker, et al. 2023. “The Multi-Omics Single-Cell Landscape of Sinus Mucosa in Uncontrolled Severe Chronic Rhinosinusitis with Nasal Polyps.” CLINICAL IMMUNOLOGY 256. https://doi.org/10.1016/j.clim.2023.109791.
Chicago author-date (all authors)
Xu, Zhaofeng, Yanran Huang, Tim Meese, Sharon Van Nevel, Gabriële Holtappels, Stijn Vanhee, Barbara M. Bröker, Zhengqi Li, Ellen De Meester, Natalie De Ruyck, Thibaut Van Zele, Philippe Gevaert, Filip Van Nieuwerburgh, Luo Zhang, Mohamed H. Shamji, Weiping Wen, Nan Zhang, and Claus Bachert. 2023. “The Multi-Omics Single-Cell Landscape of Sinus Mucosa in Uncontrolled Severe Chronic Rhinosinusitis with Nasal Polyps.” CLINICAL IMMUNOLOGY 256. doi:10.1016/j.clim.2023.109791.
Vancouver
1.
Xu Z, Huang Y, Meese T, Van Nevel S, Holtappels G, Vanhee S, et al. The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps. CLINICAL IMMUNOLOGY. 2023;256.
IEEE
[1]
Z. Xu et al., “The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps,” CLINICAL IMMUNOLOGY, vol. 256, 2023.
@article{01HBR296HRGWY8J63S16AQASAA,
  abstract     = {{Uncontrolled severe chronic rhinosinusitis with nasal polyps (CRSwNP) is associated with elevated levels of type 2 inflammatory cytokines and raised immunoglobulin concentrations in nasal polyp tissue. By using single-cell RNA sequencing, transcriptomics, surface proteomics, and T cell and B cell receptor sequencing, we found the predominant cell types in nasal polyps were shifted from epithelial and mesenchymal cells to inflammatory cells compared to nasal mucosa from healthy controls. Broad expansions of CD4 T effector memory cells, CD4 tissueresident memory T cells, CD8 T effector memory cells and all subtypes of B cells in nasal polyp tissues. The T and B cell receptor repertoires were skewed in NP. This study highlights the deviated immune response and remodeling mechanisms that contribute to the pathogenesis of uncontrolled severe CRSwNP.
Clinical implications: We identified differences in the cellular compositions, transcriptomes, proteomes, and deviations in the immune profiles of T cell and B cell receptors as well as alterations in the intercellular communications in uncontrolled severe CRSwNP patients versus healthy controls, which might help to define potential therapeutic targets in the future.}},
  articleno    = {{109791}},
  author       = {{Xu, Zhaofeng and Huang, Yanran and Meese, Tim and Van Nevel, Sharon and Holtappels, Gabriële and Vanhee, Stijn and Bröker, Barbara M. and Li, Zhengqi and De Meester, Ellen and De Ruyck, Natalie and Van Zele, Thibaut and Gevaert, Philippe and Van Nieuwerburgh, Filip and Zhang, Luo and Shamji, Mohamed H. and Wen, Weiping and Zhang, Nan and Bachert, Claus}},
  issn         = {{1521-6616}},
  journal      = {{CLINICAL IMMUNOLOGY}},
  keywords     = {{Immunology,Immunology and Allergy,Chronic rhinosinusitis with nasal polyps,scRNA-seq,Immune profiles,Tissue remodeling,Type 2 immune responses,DIFFERENTIAL EXPRESSION ANALYSIS,T-CELLS,SUBSETS,TISSUE,SUBPOPULATION,ACTIVATION,MIGRATION,PACKAGE,IGE}},
  language     = {{eng}},
  pages        = {{14}},
  title        = {{The multi-omics single-cell landscape of sinus mucosa in uncontrolled severe chronic rhinosinusitis with nasal polyps}},
  url          = {{http://doi.org/10.1016/j.clim.2023.109791}},
  volume       = {{256}},
  year         = {{2023}},
}
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