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Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana

Stefania Morales (UGent) , Joris Jourquin (UGent) , Frederic Coppé, Lorena Lopez Galvis (UGent) , Tom De Smet (UGent) , Alaeddine Safi (UGent) , Maria Njo (UGent) , Cara A. Griffiths, John D. Sidda, James S. O. Mccullagh, et al.
(2023) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 120(40).
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Abstract
Plant roots explore the soil for water and nutrients, thereby determining plant fitness and agricultural yield, as well as determining ground substructure, water levels, and global carbon sequestration. The colonization of the soil requires investment of carbon and energy, but how sugar and energy signaling are integrated with root branching is unknown. Here, we show through combined genetic and chemical modulation of signaling pathways that the sugar small-molecule signal, trehalose-6-phosphate (T6P) regulates root branching through master kinases SNF1-related kinase-1 (SnRK1) and Target of Rapamycin (TOR) and with the involvement of the plant hormone auxin. Increase of T6P levels both via genetic targeting in lateral root (LR) founder cells and through light-activated release of the presignaling T6P-precursor reveals that T6P increases root branching through coordinated inhibition of SnRK1 and activation of TOR. Auxin, the master regulator of LR formation, impacts this T6P function by transcriptionally down-regulating the T6P-degrader trehalose phosphate phosphatase B in LR cells. Our results reveal a regulatory energy-balance network for LR formation that links the ‘sugar signal’ T6P to both SnRK1 and TOR downstream of auxin.
Keywords
SnRK1, TOR, energy-balance, lateral root formation, trehalose-6-phosphate signaling

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MLA
Morales, Stefania, et al. “Trehalose-6-Phosphate Signaling Regulates Lateral Root Formation in Arabidopsis Thaliana.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 120, no. 40, 2023, doi:10.1073/pnas.2302996120.
APA
Morales, S., Jourquin, J., Coppé, F., Lopez Galvis, L., De Smet, T., Safi, A., … Beeckman, T. (2023). Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 120(40). https://doi.org/10.1073/pnas.2302996120
Chicago author-date
Morales, Stefania, Joris Jourquin, Frederic Coppé, Lorena Lopez Galvis, Tom De Smet, Alaeddine Safi, Maria Njo, et al. 2023. “Trehalose-6-Phosphate Signaling Regulates Lateral Root Formation in Arabidopsis Thaliana.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 120 (40). https://doi.org/10.1073/pnas.2302996120.
Chicago author-date (all authors)
Morales, Stefania, Joris Jourquin, Frederic Coppé, Lorena Lopez Galvis, Tom De Smet, Alaeddine Safi, Maria Njo, Cara A. Griffiths, John D. Sidda, James S. O. Mccullagh, Xiaochao Xue, Benjamin G. Davis, Johan Van der Eycken, Matthew J. Paul, Patrick Van Dijck, and Tom Beeckman. 2023. “Trehalose-6-Phosphate Signaling Regulates Lateral Root Formation in Arabidopsis Thaliana.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 120 (40). doi:10.1073/pnas.2302996120.
Vancouver
1.
Morales S, Jourquin J, Coppé F, Lopez Galvis L, De Smet T, Safi A, et al. Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2023;120(40).
IEEE
[1]
S. Morales et al., “Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana,” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 120, no. 40, 2023.
@article{01HBBCAZ6600XCCV4VHB8M2CBY,
  abstract     = {{Plant roots explore the soil for water and nutrients, thereby determining plant fitness and agricultural yield, as well as determining ground substructure, water levels, and global carbon sequestration. The colonization of the soil requires investment of carbon and energy, but how sugar and energy signaling are integrated with root branching is unknown. Here, we show through combined genetic and chemical modulation of signaling pathways that the sugar small-molecule signal, trehalose-6-phosphate (T6P) regulates root branching through master kinases SNF1-related kinase-1 (SnRK1) and Target of Rapamycin (TOR) and with the involvement of the plant hormone auxin. Increase of T6P levels both via genetic targeting in lateral root (LR) founder cells and through light-activated release of the presignaling T6P-precursor reveals that T6P increases root branching through coordinated inhibition of SnRK1 and activation of TOR. Auxin, the master regulator of LR formation, impacts this T6P function by transcriptionally down-regulating the T6P-degrader trehalose phosphate phosphatase B in LR cells. Our results reveal a regulatory energy-balance network for LR formation that links the ‘sugar signal’ T6P to both SnRK1 and TOR downstream of auxin.}},
  articleno    = {{e2302996120}},
  author       = {{Morales, Stefania and Jourquin, Joris and Coppé, Frederic and Lopez Galvis, Lorena and De Smet, Tom and Safi, Alaeddine and Njo, Maria and Griffiths, Cara A. and Sidda, John D. and Mccullagh, James S. O. and Xue, Xiaochao and Davis, Benjamin G. and Van der Eycken, Johan and Paul, Matthew J. and Van Dijck, Patrick and Beeckman, Tom}},
  issn         = {{0027-8424}},
  journal      = {{PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}},
  keywords     = {{SnRK1,TOR,energy-balance,lateral root formation,trehalose-6-phosphate signaling}},
  language     = {{eng}},
  number       = {{40}},
  pages        = {{10}},
  title        = {{Trehalose-6-phosphate signaling regulates lateral root formation in Arabidopsis thaliana}},
  url          = {{http://doi.org/10.1073/pnas.2302996120}},
  volume       = {{120}},
  year         = {{2023}},
}
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