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Smoking-related dysregulation of plasma circulating microRNAs : the Rotterdam study

(2023) HUMAN GENOMICS. 17(1).
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Abstract
BackgroundMicroRNAs (miRNAs) are post-transcriptional regulators of gene expression. Differential miRNA expression, which is widely shown to be associated with the pathogenesis of various diseases, can be influenced by lifestyle factors, including smoking. This study aimed to investigate the plasma miRNA signature of smoking habits, the potential effect of smoking cessation on miRNA levels, and relate the findings with lung cancer incidence.ResultsA targeted RNA-sequencing approach measured plasma miRNA levels in 2686 participants from the population-based Rotterdam study cohort. The association between cigarette smoking (current versus never) and 591 well-expressed miRNAs was assessed via adjusted linear regression models, identifying 41 smoking-associated miRNAs that passed the Bonferroni-corrected threshold (P < 0.05/591 = 8.46 x 10(-5)). Moreover, we found 42 miRNAs with a significant association (P < 8.46 x 10(-5)) between current (reference group) and former smokers. Then, we used adjusted linear regression models to explore the effect of smoking cessation time on miRNA expression levels. The expression levels of two miRNAs were significantly different within 5 years of cessation (P < 0.05/41 = 1.22 x 10(-3)) from current smokers, while for cessation time between 5 and 15 years we found 19 miRNAs to be significantly different from current smokers, and finally, 38 miRNAs were significantly different after more than 15 years of cessation time (P < 1.22 x 10(-3)). These results imply the reversibility of the smoking effect on plasma levels of at least 38 out of the 41 smoking-miRNAs following smoking cessation. Next, we found 8 out of the 41 smoking-related miRNAs to be nominally associated (P < 0.05) with the incidence of lung cancer.ConclusionsThis study demonstrates smoking-related dysregulation of plasma miRNAs, which might have a potential for reversibility when comparing different smoking cessation groups. The identified miRNAs are involved in several cancer-related pathways and include 8 miRNAs associated with lung cancer incidence. Our results may lay the groundwork for further investigation of miRNAs as potential mechanism linking smoking, gene expression and cancer.
Keywords
Circulating miRNAs, Smoking, Smoking cessation, Lung cancer, HEALTH CONSEQUENCES, CANCER, EPIGENETICS, MIR-150-5P, EXPRESSION, DISEASE

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MLA
Karabegovic, Irma, et al. “Smoking-Related Dysregulation of Plasma Circulating MicroRNAs : The Rotterdam Study.” HUMAN GENOMICS, vol. 17, no. 1, BMC, 2023, doi:10.1186/s40246-023-00504-5.
APA
Karabegovic, I., Maas, S. C. E., Shuai, Y., Ikram, M. A., Stricker, B., Aerts, J., … Ghanbari, M. (2023). Smoking-related dysregulation of plasma circulating microRNAs : the Rotterdam study. HUMAN GENOMICS, 17(1). https://doi.org/10.1186/s40246-023-00504-5
Chicago author-date
Karabegovic, Irma, Silvana C. E. Maas, Yu Shuai, M. Arfan Ikram, Bruno Stricker, Joachim Aerts, Guy Brusselle, Lies Lahousse, Trudy Voortman, and Mohsen Ghanbari. 2023. “Smoking-Related Dysregulation of Plasma Circulating MicroRNAs : The Rotterdam Study.” HUMAN GENOMICS 17 (1). https://doi.org/10.1186/s40246-023-00504-5.
Chicago author-date (all authors)
Karabegovic, Irma, Silvana C. E. Maas, Yu Shuai, M. Arfan Ikram, Bruno Stricker, Joachim Aerts, Guy Brusselle, Lies Lahousse, Trudy Voortman, and Mohsen Ghanbari. 2023. “Smoking-Related Dysregulation of Plasma Circulating MicroRNAs : The Rotterdam Study.” HUMAN GENOMICS 17 (1). doi:10.1186/s40246-023-00504-5.
Vancouver
1.
Karabegovic I, Maas SCE, Shuai Y, Ikram MA, Stricker B, Aerts J, et al. Smoking-related dysregulation of plasma circulating microRNAs : the Rotterdam study. HUMAN GENOMICS. 2023;17(1).
IEEE
[1]
I. Karabegovic et al., “Smoking-related dysregulation of plasma circulating microRNAs : the Rotterdam study,” HUMAN GENOMICS, vol. 17, no. 1, 2023.
@article{01HASCAG6BYDKJ863RWZE3F5P3,
  abstract     = {{BackgroundMicroRNAs (miRNAs) are post-transcriptional regulators of gene expression. Differential miRNA expression, which is widely shown to be associated with the pathogenesis of various diseases, can be influenced by lifestyle factors, including smoking. This study aimed to investigate the plasma miRNA signature of smoking habits, the potential effect of smoking cessation on miRNA levels, and relate the findings with lung cancer incidence.ResultsA targeted RNA-sequencing approach measured plasma miRNA levels in 2686 participants from the population-based Rotterdam study cohort. The association between cigarette smoking (current versus never) and 591 well-expressed miRNAs was assessed via adjusted linear regression models, identifying 41 smoking-associated miRNAs that passed the Bonferroni-corrected threshold (P < 0.05/591 = 8.46 x 10(-5)). Moreover, we found 42 miRNAs with a significant association (P < 8.46 x 10(-5)) between current (reference group) and former smokers. Then, we used adjusted linear regression models to explore the effect of smoking cessation time on miRNA expression levels. The expression levels of two miRNAs were significantly different within 5 years of cessation (P < 0.05/41 = 1.22 x 10(-3)) from current smokers, while for cessation time between 5 and 15 years we found 19 miRNAs to be significantly different from current smokers, and finally, 38 miRNAs were significantly different after more than 15 years of cessation time (P < 1.22 x 10(-3)). These results imply the reversibility of the smoking effect on plasma levels of at least 38 out of the 41 smoking-miRNAs following smoking cessation. Next, we found 8 out of the 41 smoking-related miRNAs to be nominally associated (P < 0.05) with the incidence of lung cancer.ConclusionsThis study demonstrates smoking-related dysregulation of plasma miRNAs, which might have a potential for reversibility when comparing different smoking cessation groups. The identified miRNAs are involved in several cancer-related pathways and include 8 miRNAs associated with lung cancer incidence. Our results may lay the groundwork for further investigation of miRNAs as potential mechanism linking smoking, gene expression and cancer.}},
  articleno    = {{61}},
  author       = {{Karabegovic, Irma and Maas, Silvana C. E. and  Shuai, Yu and  Ikram, M. Arfan and  Stricker, Bruno and  Aerts, Joachim and Brusselle, Guy and Lahousse, Lies and  Voortman, Trudy and  Ghanbari, Mohsen}},
  issn         = {{1473-9542}},
  journal      = {{HUMAN GENOMICS}},
  keywords     = {{Circulating miRNAs,Smoking,Smoking cessation,Lung cancer,HEALTH CONSEQUENCES,CANCER,EPIGENETICS,MIR-150-5P,EXPRESSION,DISEASE}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{12}},
  publisher    = {{BMC}},
  title        = {{Smoking-related dysregulation of plasma circulating microRNAs : the Rotterdam study}},
  url          = {{http://doi.org/10.1186/s40246-023-00504-5}},
  volume       = {{17}},
  year         = {{2023}},
}

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