Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 2.60 MB
Add to list
  1. Search results
  2. Improved compact growth habit of Viol...

Improved compact growth habit of Viola × wittrockiana through Rhizobium rhizogenes transformation

Siel Desmet, ellen de keyser (UGent) , Leen Leus, Johan Van Huylenbroeck, Danny Geelen (UGent) and Emmy Dhooghe (UGent)
(2024) PLANT GROWTH REGULATION. 102(3). p.515-527
Author
Organization
Project
Abstract
Compactness is an important breeding goal in the improvement of garden pansy varieties (Viola × wittrockiana Gams.). The desired compact stature is primarily obtained through the use of chemical growth retardants. Recently, an alternative strategy for creating compact ornamentals was proposed based on transformation by wild type rhizogenic agrobacteria and the regeneration of transformed hairy roots. Here, we report on the successful transformation and creation of compact garden pansy lines. The most efficient hairy root induction was obtained by Rhizobium rhizogenes strain ATCC15834. Shoot regeneration from hairy roots was obtained using 3 mg L−1 2iP and 0.3 mg L−1 2,4-D. The presence of R. rhizogenes T-DNA genes was confirmed in the regenerated lines. These lines displayed a varying degrees of dwarfed growth. Most notably the shoot length was reduced up to 49%. Other phenotypic traits that were induced include reduced peduncle length and smaller, wrinkled flowers. Several of these Ri lines with promising compactness have been selected for further breeding towards compact garden pansy varieties that are less dependent on chemical growth regulation.
Keywords
Garden pansy, Growth regulation, Ri phenotype, Rhizobium rhizogenes, Agrobacterium rhizogenes, HAIRY ROOT INDUCTION, AGROBACTERIUM-RHIZOGENES, MEDIATED TRANSFORMATION, PLANT-REGENERATION, PCR PRIMERS, HOST RANGE, ROL-GENES, T-DNA, PLASMID, REGIONS

Downloads

  • Download
    s10725-023-01073-2.pdf
    • full text (Published version)
    • |
    • open access
    • |
    • PDF
    • |
    • 2.60 MB

Citation

Please use this url to cite or link to this publication:

MLA
Desmet, Siel, et al. “Improved Compact Growth Habit of Viola × Wittrockiana through Rhizobium Rhizogenes Transformation.” PLANT GROWTH REGULATION, vol. 102, no. 3, 2024, pp. 515–27, doi:10.1007/s10725-023-01073-2.
APA
Desmet, S., de keyser, ellen, Leus, L., Van Huylenbroeck, J., Geelen, D., & Dhooghe, E. (2024). Improved compact growth habit of Viola × wittrockiana through Rhizobium rhizogenes transformation. PLANT GROWTH REGULATION, 102(3), 515–527. https://doi.org/10.1007/s10725-023-01073-2
Chicago author-date
Desmet, Siel, ellen de keyser, Leen Leus, Johan Van Huylenbroeck, Danny Geelen, and Emmy Dhooghe. 2024. “Improved Compact Growth Habit of Viola × Wittrockiana through Rhizobium Rhizogenes Transformation.” PLANT GROWTH REGULATION 102 (3): 515–27. https://doi.org/10.1007/s10725-023-01073-2.
Chicago author-date (all authors)
Desmet, Siel, ellen de keyser, Leen Leus, Johan Van Huylenbroeck, Danny Geelen, and Emmy Dhooghe. 2024. “Improved Compact Growth Habit of Viola × Wittrockiana through Rhizobium Rhizogenes Transformation.” PLANT GROWTH REGULATION 102 (3): 515–527. doi:10.1007/s10725-023-01073-2.
Vancouver
1.
Desmet S, de keyser ellen, Leus L, Van Huylenbroeck J, Geelen D, Dhooghe E. Improved compact growth habit of Viola × wittrockiana through Rhizobium rhizogenes transformation. PLANT GROWTH REGULATION. 2024;102(3):515–27.
IEEE
[1]
S. Desmet, ellen de keyser, L. Leus, J. Van Huylenbroeck, D. Geelen, and E. Dhooghe, “Improved compact growth habit of Viola × wittrockiana through Rhizobium rhizogenes transformation,” PLANT GROWTH REGULATION, vol. 102, no. 3, pp. 515–527, 2024.
@article{01HAKTEV35MN2MHKB1G2S22YSR,
  abstract     = {{Compactness is an important breeding goal in the improvement of garden pansy varieties (Viola × wittrockiana Gams.). The desired compact stature is primarily obtained through the use of chemical growth retardants. Recently, an alternative strategy for creating compact ornamentals was proposed based on transformation by wild type rhizogenic agrobacteria and the regeneration of transformed hairy roots. Here, we report on the successful transformation and creation of compact garden pansy lines. The most efficient hairy root induction was obtained by Rhizobium rhizogenes strain ATCC15834. Shoot regeneration from hairy roots was obtained using 3 mg L−1 2iP and 0.3 mg L−1 2,4-D. The presence of R. rhizogenes T-DNA genes was confirmed in the regenerated lines. These lines displayed a varying degrees of dwarfed growth. Most notably the shoot length was reduced up to 49%. Other phenotypic traits that were induced include reduced peduncle length and smaller, wrinkled flowers. Several of these Ri lines with promising compactness have been selected for further breeding towards compact garden pansy varieties that are less dependent on chemical growth regulation.}},
  author       = {{Desmet, Siel and de keyser, ellen and Leus, Leen and Van Huylenbroeck, Johan and Geelen, Danny and Dhooghe, Emmy}},
  issn         = {{0167-6903}},
  journal      = {{PLANT GROWTH REGULATION}},
  keywords     = {{Garden pansy,Growth regulation,Ri phenotype,Rhizobium rhizogenes,Agrobacterium rhizogenes,HAIRY ROOT INDUCTION,AGROBACTERIUM-RHIZOGENES,MEDIATED TRANSFORMATION,PLANT-REGENERATION,PCR PRIMERS,HOST RANGE,ROL-GENES,T-DNA,PLASMID,REGIONS}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{515--527}},
  title        = {{Improved compact growth habit of Viola × wittrockiana through Rhizobium rhizogenes transformation}},
  url          = {{http://doi.org/10.1007/s10725-023-01073-2}},
  volume       = {{102}},
  year         = {{2024}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: