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Bacterial responses to plant antimicrobials : the case of alkannin and shikonin derivatives

Angélique Rat (UGent) , Antigoni E. Koletti, Nebojsa Rodic, Vassilios P. Papageorgiou, Anne Willems (UGent) and Andreana N. Assimopoulou
(2023) FRONTIERS IN PHARMACOLOGY. 14.
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Abstract
Alkannin, shikonin and their derivatives (A/S) are secondary metabolites produced in the roots of certain plants of the Boraginaceae family such as Lithospermum erythrorhizon Siebold & Zucc. and Alkanna tinctoria (L.) Tausch. These naphthoquinones express anti-cancer, wound healing, and antimicrobial activities. To study the interactions between endophytic bacteria isolated from A. tinctoria and the antimicrobials A/S, endophytic bacteria known to be resistant to the compounds were screened for their effect on A/S in liquid medium. Thereafter, the strain Pseudomonas sp. R-72008, was selected and tested for its ability to modify A/S in nutrient medium and minimal medium with A/S as sole carbon source. Bacterial growth was recorded, and high performance liquid chromatography-diode array and ultra-high performance liquid chromatography-electrospray ionization-mass spectrometry analyses were performed to detect and quantify metabolites. In nutrient medium inoculated with R-72008, a decrease in the amount of A/S monomers initially present was observed and correlated with an increase of A/S oligomers. Moreover, a significant decrease of initial A/S monomers in minimal medium was correlated with bacterial growth, showing for the first time that a bacterial strain, Pseudomonas sp. R-72008, was able to use the naphthoquinones A/S as sole carbon source. This study opens new perspectives on the interactions between bacteria and plant antimicrobials.
Keywords
IN-VITRO, NAPHTHOQUINONES, PIGMENTS, BIOLOGY, shikonin, Pseudomonas sp, oligomers, metabolization, naphthoquinone, bacteria, metabolites, antimicrobial activity

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Citation

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MLA
Rat, Angélique, et al. “Bacterial Responses to Plant Antimicrobials : The Case of Alkannin and Shikonin Derivatives.” FRONTIERS IN PHARMACOLOGY, vol. 14, 2023, doi:10.3389/fphar.2023.1244270.
APA
Rat, A., Koletti, A. E., Rodic, N., Papageorgiou, V. P., Willems, A., & Assimopoulou, A. N. (2023). Bacterial responses to plant antimicrobials : the case of alkannin and shikonin derivatives. FRONTIERS IN PHARMACOLOGY, 14. https://doi.org/10.3389/fphar.2023.1244270
Chicago author-date
Rat, Angélique, Antigoni E. Koletti, Nebojsa Rodic, Vassilios P. Papageorgiou, Anne Willems, and Andreana N. Assimopoulou. 2023. “Bacterial Responses to Plant Antimicrobials : The Case of Alkannin and Shikonin Derivatives.” FRONTIERS IN PHARMACOLOGY 14. https://doi.org/10.3389/fphar.2023.1244270.
Chicago author-date (all authors)
Rat, Angélique, Antigoni E. Koletti, Nebojsa Rodic, Vassilios P. Papageorgiou, Anne Willems, and Andreana N. Assimopoulou. 2023. “Bacterial Responses to Plant Antimicrobials : The Case of Alkannin and Shikonin Derivatives.” FRONTIERS IN PHARMACOLOGY 14. doi:10.3389/fphar.2023.1244270.
Vancouver
1.
Rat A, Koletti AE, Rodic N, Papageorgiou VP, Willems A, Assimopoulou AN. Bacterial responses to plant antimicrobials : the case of alkannin and shikonin derivatives. FRONTIERS IN PHARMACOLOGY. 2023;14.
IEEE
[1]
A. Rat, A. E. Koletti, N. Rodic, V. P. Papageorgiou, A. Willems, and A. N. Assimopoulou, “Bacterial responses to plant antimicrobials : the case of alkannin and shikonin derivatives,” FRONTIERS IN PHARMACOLOGY, vol. 14, 2023.
@article{01HA9XA9V4B7A4S0F7ZC29RVBA,
  abstract     = {{Alkannin, shikonin and their derivatives (A/S) are secondary metabolites produced in the roots of certain plants of the Boraginaceae family such as Lithospermum erythrorhizon Siebold & Zucc. and Alkanna tinctoria (L.) Tausch. These naphthoquinones express anti-cancer, wound healing, and antimicrobial activities. To study the interactions between endophytic bacteria isolated from A. tinctoria and the antimicrobials A/S, endophytic bacteria known to be resistant to the compounds were screened for their effect on A/S in liquid medium. Thereafter, the strain Pseudomonas sp. R-72008, was selected and tested for its ability to modify A/S in nutrient medium and minimal medium with A/S as sole carbon source. Bacterial growth was recorded, and high performance liquid chromatography-diode array and ultra-high performance liquid chromatography-electrospray ionization-mass spectrometry analyses were performed to detect and quantify metabolites. In nutrient medium inoculated with R-72008, a decrease in the amount of A/S monomers initially present was observed and correlated with an increase of A/S oligomers. Moreover, a significant decrease of initial A/S monomers in minimal medium was correlated with bacterial growth, showing for the first time that a bacterial strain, Pseudomonas sp. R-72008, was able to use the naphthoquinones A/S as sole carbon source. This study opens new perspectives on the interactions between bacteria and plant antimicrobials.}},
  articleno    = {{1244270}},
  author       = {{Rat, Angélique and  Koletti, Antigoni E. and  Rodic, Nebojsa and  Papageorgiou, Vassilios P. and Willems, Anne and  Assimopoulou, Andreana N.}},
  issn         = {{1663-9812}},
  journal      = {{FRONTIERS IN PHARMACOLOGY}},
  keywords     = {{IN-VITRO,NAPHTHOQUINONES,PIGMENTS,BIOLOGY,shikonin,Pseudomonas sp,oligomers,metabolization,naphthoquinone,bacteria,metabolites,antimicrobial activity}},
  language     = {{eng}},
  pages        = {{11}},
  title        = {{Bacterial responses to plant antimicrobials : the case of alkannin and shikonin derivatives}},
  url          = {{http://doi.org/10.3389/fphar.2023.1244270}},
  volume       = {{14}},
  year         = {{2023}},
}
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