Increasing the overall proteome coverage by combining protein digestion by Tryp-N and trypsin
- Author
- Jade Hawksworth (UGent) , Lode Denolf (UGent) , Evy Timmerman (UGent) and Kris Gevaert (UGent)
- Organization
- Abstract
- Mass spectrometry-based proteomics combining more than one protease in parallel facilitates the identification of more peptides and proteins than when a single protease is used. Trypsin cleaves proteins C-terminally to arginine and lysine, while its mirroring protease Tryp-N cleaves N-terminally to the same amino acids. Here, we combine trypsin and Tryp-N with the commercially available S-Trap columns, which purify protein samples and catalyze digestion. Comparison of trypsin or Tryp-N coupled with S-Trap columns demonstrates plasma and cell lysate proteins unique to one protease. We thus suggest the use of both proteases in a complementary manner to obtain deeper proteome coverage.
- Keywords
- Proteases, Trypsin, Tryp-N, S-Trap, Mass spectrometry
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01H9K9990K228FB1GQ9PTJWM3A
- MLA
- Hawksworth, Jade, et al. “Increasing the Overall Proteome Coverage by Combining Protein Digestion by Tryp-N and Trypsin.” Mass Spectrometry-Based Proteomics, edited by Kris Gevaert, vol. 2718, Humana, 2023, pp. 1–10, doi:10.1007/978-1-0716-3457-8_1.
- APA
- Hawksworth, J., Denolf, L., Timmerman, E., & Gevaert, K. (2023). Increasing the overall proteome coverage by combining protein digestion by Tryp-N and trypsin. In K. Gevaert (Ed.), Mass spectrometry-based proteomics (Vol. 2718, pp. 1–10). https://doi.org/10.1007/978-1-0716-3457-8_1
- Chicago author-date
- Hawksworth, Jade, Lode Denolf, Evy Timmerman, and Kris Gevaert. 2023. “Increasing the Overall Proteome Coverage by Combining Protein Digestion by Tryp-N and Trypsin.” In Mass Spectrometry-Based Proteomics, edited by Kris Gevaert, 2718:1–10. New York, NY: Humana. https://doi.org/10.1007/978-1-0716-3457-8_1.
- Chicago author-date (all authors)
- Hawksworth, Jade, Lode Denolf, Evy Timmerman, and Kris Gevaert. 2023. “Increasing the Overall Proteome Coverage by Combining Protein Digestion by Tryp-N and Trypsin.” In Mass Spectrometry-Based Proteomics, ed by. Kris Gevaert, 2718:1–10. New York, NY: Humana. doi:10.1007/978-1-0716-3457-8_1.
- Vancouver
- 1.Hawksworth J, Denolf L, Timmerman E, Gevaert K. Increasing the overall proteome coverage by combining protein digestion by Tryp-N and trypsin. In: Gevaert K, editor. Mass spectrometry-based proteomics. New York, NY: Humana; 2023. p. 1–10.
- IEEE
- [1]J. Hawksworth, L. Denolf, E. Timmerman, and K. Gevaert, “Increasing the overall proteome coverage by combining protein digestion by Tryp-N and trypsin,” in Mass spectrometry-based proteomics, vol. 2718, K. Gevaert, Ed. New York, NY: Humana, 2023, pp. 1–10.
@incollection{01H9K9990K228FB1GQ9PTJWM3A,
abstract = {{Mass spectrometry-based proteomics combining more than one protease in parallel facilitates the identification of more peptides and proteins than when a single protease is used. Trypsin cleaves proteins C-terminally to arginine and lysine, while its mirroring protease Tryp-N cleaves N-terminally to the same amino acids. Here, we combine trypsin and Tryp-N with the commercially available S-Trap columns, which purify protein samples and catalyze digestion. Comparison of trypsin or Tryp-N coupled with S-Trap columns demonstrates plasma and cell lysate proteins unique to one protease. We thus suggest the use of both proteases in a complementary manner to obtain deeper proteome coverage.}},
author = {{Hawksworth, Jade and Denolf, Lode and Timmerman, Evy and Gevaert, Kris}},
booktitle = {{Mass spectrometry-based proteomics}},
editor = {{Gevaert, Kris}},
isbn = {{9781071634561}},
issn = {{1064-3745}},
keywords = {{Proteases,Trypsin,Tryp-N,S-Trap,Mass spectrometry}},
language = {{eng}},
pages = {{1--10}},
publisher = {{Humana}},
series = {{Methods in Molecular Biology}},
title = {{Increasing the overall proteome coverage by combining protein digestion by Tryp-N and trypsin}},
url = {{http://doi.org/10.1007/978-1-0716-3457-8_1}},
volume = {{2718}},
year = {{2023}},
}
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