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Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm

(2023) BIOFILM. 6.
Author
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Abstract
Chronic wound management is extremely challenging because of the persistence of biofilm-forming pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, which are the prevailing bacterial species that coinfect chronic wounds. Phage therapy has gained an increased interest to treat biofilm-associated infections, namely when combined with antibiotics. Here, we tested the effect of gentamicin as a co-adjuvant of phages in a dual species-biofilm wound model formed on artificial dermis. The biofilm-killing capacity of the tested treatments was significantly increased when phages were combined with gentamicin and applied multiple times as multiple dose (three doses, every 8 h). Our results suggest that gentamycin is an effective adjuvant of phage therapy particularly when applied simultaneously with phages and in three consecutive doses. The multiple and simultaneous dose treatment seems to be essential to avoid bacterial resistance development to each of the antimicrobial agents.
Keywords
Cell Biology, Molecular Biology, Applied Microbiology and Biotechnology, Microbiology, Pseudomonas aeruginosa, Staphylococcus aureus, Phage-antibiotic combination, Gentamicin, Phage-antibiotic synergy (PAS), Sequential treatment, Dual-species biofilm, Artificial wound model, Wound infection, PSEUDOMONAS-AERUGINOSA, STAPHYLOCOCCUS-AUREUS, BACTERIA, GROWTH

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Citation

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MLA
Akturk, Ergun, et al. “Combining Phages and Antibiotic to Enhance Antibiofilm Efficacy against an in Vitro Dual Species Wound Biofilm.” BIOFILM, vol. 6, 2023, p. 100147, doi:10.1016/j.bioflm.2023.100147.
APA
Akturk, E., Melo, L. D. R., Oliveira, H., Crabbé, A., Coenye, T., & Azeredo, J. (2023). Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm. BIOFILM, 6, 100147. https://doi.org/10.1016/j.bioflm.2023.100147
Chicago author-date
Akturk, Ergun, Luís D.R. Melo, Hugo Oliveira, Aurélie Crabbé, Tom Coenye, and Joana Azeredo. 2023. “Combining Phages and Antibiotic to Enhance Antibiofilm Efficacy against an in Vitro Dual Species Wound Biofilm.” BIOFILM 6: 100147. https://doi.org/10.1016/j.bioflm.2023.100147.
Chicago author-date (all authors)
Akturk, Ergun, Luís D.R. Melo, Hugo Oliveira, Aurélie Crabbé, Tom Coenye, and Joana Azeredo. 2023. “Combining Phages and Antibiotic to Enhance Antibiofilm Efficacy against an in Vitro Dual Species Wound Biofilm.” BIOFILM 6: 100147. doi:10.1016/j.bioflm.2023.100147.
Vancouver
1.
Akturk E, Melo LDR, Oliveira H, Crabbé A, Coenye T, Azeredo J. Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm. BIOFILM. 2023;6:100147.
IEEE
[1]
E. Akturk, L. D. R. Melo, H. Oliveira, A. Crabbé, T. Coenye, and J. Azeredo, “Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm,” BIOFILM, vol. 6, p. 100147, 2023.
@article{01H93682GVS7S6DP609AQ5M3AD,
  abstract     = {{Chronic wound management is extremely challenging because of the persistence of biofilm-forming pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, which are the prevailing bacterial species that coinfect chronic wounds. Phage therapy has gained an increased interest to treat biofilm-associated infections, namely when combined with antibiotics. Here, we tested the effect of gentamicin as a co-adjuvant of phages in a dual species-biofilm wound model formed on artificial dermis. The biofilm-killing capacity of the tested treatments was significantly increased when phages were combined with gentamicin and applied multiple times as multiple dose (three doses, every 8 h). Our results suggest that gentamycin is an effective adjuvant of phage therapy particularly when applied simultaneously with phages and in three consecutive doses. The multiple and simultaneous dose treatment seems to be essential to avoid bacterial resistance development to each of the antimicrobial agents.}},
  articleno    = {{100147}},
  author       = {{Akturk, Ergun and Melo, Luís D.R. and Oliveira, Hugo and Crabbé, Aurélie and Coenye, Tom and Azeredo, Joana}},
  issn         = {{2590-2075}},
  journal      = {{BIOFILM}},
  keywords     = {{Cell Biology,Molecular Biology,Applied Microbiology and Biotechnology,Microbiology,Pseudomonas aeruginosa,Staphylococcus aureus,Phage-antibiotic combination,Gentamicin,Phage-antibiotic synergy (PAS),Sequential treatment,Dual-species biofilm,Artificial wound model,Wound infection,PSEUDOMONAS-AERUGINOSA,STAPHYLOCOCCUS-AUREUS,BACTERIA,GROWTH}},
  language     = {{eng}},
  pages        = {{7}},
  title        = {{Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm}},
  url          = {{http://doi.org/10.1016/j.bioflm.2023.100147}},
  volume       = {{6}},
  year         = {{2023}},
}

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