Transport by circulating myeloid cells drives liposomal accumulation in inflamed synovium
- Author
- Joke Deprez (UGent) , Rein Verbeke (UGent) , Sofie Meulewaeter (UGent) , Ilke Aernout (UGent) , Heleen Dewitte (UGent) , Tine Decruy (UGent) , Julie Coudenys (UGent) , Julie Van Duyse (UGent) , Gert Van Isterdael (UGent) , Dan Peer, Roy van der Meel, Stefaan De Smedt (UGent) , Peggy Jacques (UGent) , Dirk Elewaut (UGent) and Ine Lentacker (UGent)
- Organization
- Project
-
- Liposome loaded microbubbles for the ultrasound triggered release of corticosteroids and TNF-α siRNA in the inflamed synovial tissue during rheumatoid arthritis.
- Understanding and modulating the pro-inflammatory activity of lipid nanoparticles (LNP) in the COVID-19 mRNA vaccines: towards the development of more effective LNP formulations.
- Exploration of an mRNA based nanovaccine platform and immunopeptidomics for the development of more effective tuberculosis vaccines.
- Further development of mRNA Galsomes as nanovaccines for cancer immunotherapy: combination with checkpoint inhibition and translation to the clinic.
- High intensity focused ultrasound and mRNA nanomedicines: a new strategy to induce and boost anti-tumor immunity
- The role of innate-like T cells in combined gut-joint disease in spondyloarthritis
- How T cell autoreactivity develops in early rheumatoid arthritis: the smoking gun hypothesis
- Mechanoinflammation in arthritic disease
- Proteomics-derived epitopes for dramatically improved anticancer and antibacterial vaccine development
- Role of endogenous NKT ligands induced by Endoplasmic Reticulum stress in NASH-driven hepatocellular carcinoma
- Cofunding core facility - Animalarium,Radiological and Radiobiological Techniques, Histology Core
- Abstract
- The therapeutic potential of liposomes to deliver drugs into inflamed tissue is well documented. Liposomes are believed to largely transport drugs into inflamed joints by selective extravasation through endothelial gaps at the inflammatory sites, known as the enhanced permeation and retention effect. However, the potential of blood-circulating myeloid cells for the uptake and delivery of liposomes has been largely overlooked. Here we show that myeloid cells can transport liposomes to inflammatory sites in a collagen-induced arthritis model. It is shown that the selective depletion of the circulating myeloid cells reduces the accumulation of liposomes up to 50-60%, suggesting that myeloid-cell-mediated transport accounts for more than half of liposomal accumulation in inflamed regions. Although it is widely believed that PEGylation inhibits premature liposome clearance by the mononuclear phagocytic system, our data show that the long blood circulation times of PEGylated liposomes rather favours uptake by myeloid cells. This challenges the prevailing theory that synovial liposomal accumulation is primarily due to the enhanced permeation and retention effect and highlights the potential for other pathways of delivery in inflammatory diseases. PEGylated liposomal accumulation in inflamed regions has mainly been attributed to the enhanced permeation and retention effect. An arthritis model that chemotactically attracted myeloid cells shows that monocytes and neutrophils play an essential role in liposome delivery towards inflamed joints.
Downloads
-
Deprez et al AAM.pdf
- full text (Accepted manuscript)
- |
- open access
- |
- |
- 4.87 MB
-
(...).pdf
- full text (Published version)
- |
- UGent only
- |
- |
- 5.98 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01H8GVMRZM95PYG0CJRV0YTHJ6
- MLA
- Deprez, Joke, et al. “Transport by Circulating Myeloid Cells Drives Liposomal Accumulation in Inflamed Synovium.” NATURE NANOTECHNOLOGY, vol. 18, 2023, pp. 1341–50, doi:10.1038/s41565-023-01444-w.
- APA
- Deprez, J., Verbeke, R., Meulewaeter, S., Aernout, I., Dewitte, H., Decruy, T., … Lentacker, I. (2023). Transport by circulating myeloid cells drives liposomal accumulation in inflamed synovium. NATURE NANOTECHNOLOGY, 18, 1341–1350. https://doi.org/10.1038/s41565-023-01444-w
- Chicago author-date
- Deprez, Joke, Rein Verbeke, Sofie Meulewaeter, Ilke Aernout, Heleen Dewitte, Tine Decruy, Julie Coudenys, et al. 2023. “Transport by Circulating Myeloid Cells Drives Liposomal Accumulation in Inflamed Synovium.” NATURE NANOTECHNOLOGY 18: 1341–50. https://doi.org/10.1038/s41565-023-01444-w.
- Chicago author-date (all authors)
- Deprez, Joke, Rein Verbeke, Sofie Meulewaeter, Ilke Aernout, Heleen Dewitte, Tine Decruy, Julie Coudenys, Julie Van Duyse, Gert Van Isterdael, Dan Peer, Roy van der Meel, Stefaan De Smedt, Peggy Jacques, Dirk Elewaut, and Ine Lentacker. 2023. “Transport by Circulating Myeloid Cells Drives Liposomal Accumulation in Inflamed Synovium.” NATURE NANOTECHNOLOGY 18: 1341–1350. doi:10.1038/s41565-023-01444-w.
- Vancouver
- 1.Deprez J, Verbeke R, Meulewaeter S, Aernout I, Dewitte H, Decruy T, et al. Transport by circulating myeloid cells drives liposomal accumulation in inflamed synovium. NATURE NANOTECHNOLOGY. 2023;18:1341–50.
- IEEE
- [1]J. Deprez et al., “Transport by circulating myeloid cells drives liposomal accumulation in inflamed synovium,” NATURE NANOTECHNOLOGY, vol. 18, pp. 1341–1350, 2023.
@article{01H8GVMRZM95PYG0CJRV0YTHJ6,
abstract = {{The therapeutic potential of liposomes to deliver drugs into inflamed tissue is well documented. Liposomes are believed to largely transport drugs into inflamed joints by selective extravasation through endothelial gaps at the inflammatory sites, known as the enhanced permeation and retention effect. However, the potential of blood-circulating myeloid cells for the uptake and delivery of liposomes has been largely overlooked. Here we show that myeloid cells can transport liposomes to inflammatory sites in a collagen-induced arthritis model. It is shown that the selective depletion of the circulating myeloid cells reduces the accumulation of liposomes up to 50-60%, suggesting that myeloid-cell-mediated transport accounts for more than half of liposomal accumulation in inflamed regions. Although it is widely believed that PEGylation inhibits premature liposome clearance by the mononuclear phagocytic system, our data show that the long blood circulation times of PEGylated liposomes rather favours uptake by myeloid cells. This challenges the prevailing theory that synovial liposomal accumulation is primarily due to the enhanced permeation and retention effect and highlights the potential for other pathways of delivery in inflammatory diseases.
PEGylated liposomal accumulation in inflamed regions has mainly been attributed to the enhanced permeation and retention effect. An arthritis model that chemotactically attracted myeloid cells shows that monocytes and neutrophils play an essential role in liposome delivery towards inflamed joints.}},
author = {{Deprez, Joke and Verbeke, Rein and Meulewaeter, Sofie and Aernout, Ilke and Dewitte, Heleen and Decruy, Tine and Coudenys, Julie and Van Duyse, Julie and Van Isterdael, Gert and Peer, Dan and van der Meel, Roy and De Smedt, Stefaan and Jacques, Peggy and Elewaut, Dirk and Lentacker, Ine}},
issn = {{1748-3387}},
journal = {{NATURE NANOTECHNOLOGY}},
language = {{eng}},
pages = {{1341--1350}},
title = {{Transport by circulating myeloid cells drives liposomal accumulation in inflamed synovium}},
url = {{http://doi.org/10.1038/s41565-023-01444-w}},
volume = {{18}},
year = {{2023}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: