Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) coiled-coil systems
- Author
- Dorien Aerssens, Laia Miret Casals (UGent) , D. Gomez, D. Sousa-Neves, Y. Levy, M. De Vleesschouwer, Alex Manicardi (UGent) and Annemieke Madder (UGent)
- Organization
- Project
- Abstract
- We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)(3) sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene-pyrene stacking.
- Keywords
- X-RAY-STRUCTURE, CRYSTAL-STRUCTURE, NUCLEIC-ACID, PROTEIN, PARALLEL, PYRENE, MODEL, PROBE, DESIGN, ZIPPER
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01H6XKSW1MG98YE40N1JRBBE7V
- MLA
- Aerssens, Dorien, et al. “Photo-Induced Crosslinking Uncovers an Antiparallel Strand Orientation in Heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) Coiled-Coil Systems.” ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 21, no. 27, Royal Soc Chemistry, 2023, pp. 5648–57, doi:10.1039/d2ob02181a.
- APA
- Aerssens, D., Miret Casals, L., Gomez, D., Sousa-Neves, D., Levy, Y., De Vleesschouwer, M., … Madder, A. (2023). Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) coiled-coil systems. ORGANIC & BIOMOLECULAR CHEMISTRY, 21(27), 5648–5657. https://doi.org/10.1039/d2ob02181a
- Chicago author-date
- Aerssens, Dorien, Laia Miret Casals, D. Gomez, D. Sousa-Neves, Y. Levy, M. De Vleesschouwer, Alex Manicardi, and Annemieke Madder. 2023. “Photo-Induced Crosslinking Uncovers an Antiparallel Strand Orientation in Heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) Coiled-Coil Systems.” ORGANIC & BIOMOLECULAR CHEMISTRY 21 (27): 5648–57. https://doi.org/10.1039/d2ob02181a.
- Chicago author-date (all authors)
- Aerssens, Dorien, Laia Miret Casals, D. Gomez, D. Sousa-Neves, Y. Levy, M. De Vleesschouwer, Alex Manicardi, and Annemieke Madder. 2023. “Photo-Induced Crosslinking Uncovers an Antiparallel Strand Orientation in Heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) Coiled-Coil Systems.” ORGANIC & BIOMOLECULAR CHEMISTRY 21 (27): 5648–5657. doi:10.1039/d2ob02181a.
- Vancouver
- 1.Aerssens D, Miret Casals L, Gomez D, Sousa-Neves D, Levy Y, De Vleesschouwer M, et al. Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) coiled-coil systems. ORGANIC & BIOMOLECULAR CHEMISTRY. 2023;21(27):5648–57.
- IEEE
- [1]D. Aerssens et al., “Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) coiled-coil systems,” ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 21, no. 27, pp. 5648–5657, 2023.
@article{01H6XKSW1MG98YE40N1JRBBE7V,
abstract = {{We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)(3) sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene-pyrene stacking.}},
author = {{Aerssens, Dorien and Miret Casals, Laia and Gomez, D. and Sousa-Neves, D. and Levy, Y. and De Vleesschouwer, M. and Manicardi, Alex and Madder, Annemieke}},
issn = {{1477-0520}},
journal = {{ORGANIC & BIOMOLECULAR CHEMISTRY}},
keywords = {{X-RAY-STRUCTURE,CRYSTAL-STRUCTURE,NUCLEIC-ACID,PROTEIN,PARALLEL,PYRENE,MODEL,PROBE,DESIGN,ZIPPER}},
language = {{eng}},
number = {{27}},
pages = {{5648--5657}},
publisher = {{Royal Soc Chemistry}},
title = {{Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)(3)/(KIAALKE)(3) and (EIAALEK)(3)/(RIAALRE)(3) coiled-coil systems}},
url = {{http://doi.org/10.1039/d2ob02181a}},
volume = {{21}},
year = {{2023}},
}
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