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Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cyst: methodological framework, acquired insights and future perspectives.

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Abstract
Fourier-transform infrared (FTIR) spectroscopy is a spectrochemical technique able to retrieve macromolecular information from organic materials. When combined with a microscope (micro-FTIR), the bulk (geo)chemical composition of single specimen dinoflagellate cysts (dinocysts) can be determined. Micro-FTIR studies on dinocysts are sparse, partially due to the complexity of the method in terms of possible disruptive variables and the interpretability of the data it provides. Nevertheless, it can be a powerful, rapid, and relatively low-cost tool for retrieving macromolecular information from small organic particles. That is, if a certain degree of methodological insights and control can be ensured. This recently led to the development of a methodological framework (Meyvisch et al. 2021) wherein a wide array of variables are discussed. The result of this study is a method based on attenuated total reflection (ATR) micro-FTIR spectroscopy which allows for the collection of robust spectrochemical datasets, and which will be presented here. Earlier micro-FTIR studies on dinocysts have explored the potential of the method to be used as a chemotaxonomical tool (Bogus et al. 2012), a proxy for trophic affinity (Bogus et al. 2014) and to assess preservation of organic matter in oxic and sulphidic depositional environments (Versteegh et al. 2020). Within the light of the later developed methodological framework, some of these authors’ findings are re- evaluated and supplemented with more elaborate and robust datasets. The overview provided on the current state of this – relatively underexplored – research domain perfectly sets the stage to discuss its future perspectives, with a focus on providing answers to major standing questions regarding the (geo)chemical nature and variability of dinoflagellate cyst walls. Finally, other useful and complementary analytical methods which can contribute to elucidating these problems will be briefly discussed.
Keywords
Fourier-transform infrared spectroscopy, organic-walled dinoflagellate cysts, cyst wall composition, methodology, chemometrics

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MLA
Meyvisch, Pjotr, et al. “Recent Advances in Infrared Spectroscopy Applied to Single Specimen Dinoflagellate Cyst: Methodological Framework, Acquired Insights and Future Perspectives.” Modern and Fossil Dinoflagellates, 12th International Conference, Abstracts, 2022, pp. 141–42.
APA
Meyvisch, P., Neil Mertens, K., Vrielinck, H., Versteegh, G. J. M., Gurdebeke, P., & Louwye, S. (2022). Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cyst: methodological framework, acquired insights and future perspectives. Modern and Fossil Dinoflagellates, 12th International Conference, Abstracts, 141–142.
Chicago author-date
Meyvisch, Pjotr, Kenneth Neil Mertens, Henk Vrielinck, Gerard J. M. Versteegh, Pieter Gurdebeke, and Stephen Louwye. 2022. “Recent Advances in Infrared Spectroscopy Applied to Single Specimen Dinoflagellate Cyst: Methodological Framework, Acquired Insights and Future Perspectives.” In Modern and Fossil Dinoflagellates, 12th International Conference, Abstracts, 141–42.
Chicago author-date (all authors)
Meyvisch, Pjotr, Kenneth Neil Mertens, Henk Vrielinck, Gerard J. M. Versteegh, Pieter Gurdebeke, and Stephen Louwye. 2022. “Recent Advances in Infrared Spectroscopy Applied to Single Specimen Dinoflagellate Cyst: Methodological Framework, Acquired Insights and Future Perspectives.” In Modern and Fossil Dinoflagellates, 12th International Conference, Abstracts, 141–142.
Vancouver
1.
Meyvisch P, Neil Mertens K, Vrielinck H, Versteegh GJM, Gurdebeke P, Louwye S. Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cyst: methodological framework, acquired insights and future perspectives. In: Modern and Fossil Dinoflagellates, 12th International Conference, Abstracts. 2022. p. 141–2.
IEEE
[1]
P. Meyvisch, K. Neil Mertens, H. Vrielinck, G. J. M. Versteegh, P. Gurdebeke, and S. Louwye, “Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cyst: methodological framework, acquired insights and future perspectives.,” in Modern and Fossil Dinoflagellates, 12th International Conference, Abstracts, Las Palmas de Gran Canaria, Spain, 2022, pp. 141–142.
@inproceedings{01H52D7ECEE3K8HZ03PJ48K9BX,
  abstract     = {{Fourier-transform infrared (FTIR) spectroscopy is a spectrochemical technique able to retrieve macromolecular information from organic materials. When combined with a microscope (micro-FTIR), the bulk (geo)chemical composition of single specimen dinoflagellate cysts (dinocysts) can be determined. Micro-FTIR studies on dinocysts are sparse, partially due to the complexity of the method in terms of possible disruptive variables and the interpretability of the data it provides. Nevertheless, it can be a powerful, rapid, and relatively low-cost tool for retrieving macromolecular information from small organic particles. That is, if a certain degree of methodological insights and control can be ensured. This recently led to the development of a methodological framework (Meyvisch et al. 2021) wherein a wide array of variables are discussed. The result of this study is a method based on attenuated total reflection (ATR) micro-FTIR spectroscopy which allows for the collection of robust spectrochemical datasets, and which will be presented here.
Earlier micro-FTIR studies on dinocysts have explored the potential of the method to be used as a chemotaxonomical tool (Bogus et al. 2012), a proxy for trophic affinity (Bogus et al. 2014) and to assess preservation of organic matter in oxic and sulphidic depositional environments (Versteegh et al. 2020). Within the light of the later developed methodological framework, some of these authors’ findings are re- evaluated and supplemented with more elaborate and robust datasets.
The overview provided on the current state of this – relatively underexplored – research domain perfectly sets the stage to discuss its future perspectives, with a focus on providing answers to major standing questions regarding the (geo)chemical nature and variability of dinoflagellate cyst walls. Finally, other useful and complementary analytical methods which can contribute to elucidating these problems will be briefly discussed.}},
  articleno    = {{7.05}},
  author       = {{Meyvisch, Pjotr and Neil Mertens, Kenneth and Vrielinck, Henk and Versteegh, Gerard J. M. and Gurdebeke, Pieter and Louwye, Stephen}},
  booktitle    = {{Modern and Fossil Dinoflagellates, 12th International Conference, Abstracts}},
  keywords     = {{Fourier-transform infrared spectroscopy,organic-walled dinoflagellate cysts,cyst wall composition,methodology,chemometrics}},
  language     = {{eng}},
  location     = {{Las Palmas de Gran Canaria, Spain}},
  pages        = {{7.05:141--7.05:142}},
  title        = {{Recent advances in infrared spectroscopy applied to single specimen dinoflagellate cyst: methodological framework, acquired insights and future perspectives.}},
  url          = {{https://dino12conference.com/wp-content/uploads/2022/07/LIBRO-DE-RESUMENES-DINO12-definitivo.pdf}},
  year         = {{2022}},
}