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Gravitational effects on fibroblasts’ function in relation to wound healing

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Abstract
The spaceflight environment imposes risks for maintaining a healthy skin function as the observed delayed wound healing can contribute to increased risks of infection. To counteract delayed wound healing in space, a better understanding of the fibroblasts' reaction to altered gravity levels is needed. In this paper, we describe experiments that were carried out at the Large Diameter Centrifuge located in ESA-ESTEC as part of the ESA Academy 2021 Spin Your Thesis! Campaign. We exposed dermal fibroblasts to a set of altered gravity levels, including transitions between simulated microgravity and hypergravity. The addition of the stress hormone cortisol to the cell culture medium was done to account for possible interaction effects of gravity and cortisol exposure. Results show a main impact of cortisol on the secretion of pro-inflammatory cytokines as well as extracellular matrix proteins. Altered gravity mostly induced a delay in cellular migration and changes in mechanosensitive cell structures. Furthermore, 20 x g hypergravity transitions induced changes in nuclear morphology. These findings provide insights into the effect of gravity transitions on the fibroblasts' function related to wound healing, which may be useful for the development of countermeasures.
Keywords
Space and Planetary Science, Physics and Astronomy (miscellaneous), Agricultural and Biological Sciences (miscellaneous), Biochemistry, Genetics and Molecular Biology (miscellaneous), Materials Science (miscellaneous), Medicine (miscellaneous)

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MLA
Radstake, Wilhelmina, et al. “Gravitational Effects on Fibroblasts’ Function in Relation to Wound Healing.” NPJ MICROGRAVITY, vol. 9, no. 1, Springer Science and Business Media LLC, 2023, doi:10.1038/s41526-023-00286-z.
APA
Radstake, W., Gautam, K., Ferreira Da Silva Miranda, S., Van Rompay, C., Vermeesen, R., Tabury, K., … Baselet, B. (2023). Gravitational effects on fibroblasts’ function in relation to wound healing. NPJ MICROGRAVITY, 9(1). https://doi.org/10.1038/s41526-023-00286-z
Chicago author-date
Radstake, Wilhelmina, Kiran Gautam, Silvana Ferreira Da Silva Miranda, Cynthia Van Rompay, Randy Vermeesen, Kevin Tabury, Mieke Verslegers, et al. 2023. “Gravitational Effects on Fibroblasts’ Function in Relation to Wound Healing.” NPJ MICROGRAVITY 9 (1). https://doi.org/10.1038/s41526-023-00286-z.
Chicago author-date (all authors)
Radstake, Wilhelmina, Kiran Gautam, Silvana Ferreira Da Silva Miranda, Cynthia Van Rompay, Randy Vermeesen, Kevin Tabury, Mieke Verslegers, Alan Dowson, Jeffrey Gorissen, Jack J. W. A. van Loon, Nigel D. L. Savage, Sarah Baatout, and Bjorn Baselet. 2023. “Gravitational Effects on Fibroblasts’ Function in Relation to Wound Healing.” NPJ MICROGRAVITY 9 (1). doi:10.1038/s41526-023-00286-z.
Vancouver
1.
Radstake W, Gautam K, Ferreira Da Silva Miranda S, Van Rompay C, Vermeesen R, Tabury K, et al. Gravitational effects on fibroblasts’ function in relation to wound healing. NPJ MICROGRAVITY. 2023;9(1).
IEEE
[1]
W. Radstake et al., “Gravitational effects on fibroblasts’ function in relation to wound healing,” NPJ MICROGRAVITY, vol. 9, no. 1, 2023.
@article{01H488SEFMD939AQR94V6SW2JX,
  abstract     = {{The spaceflight environment imposes risks for maintaining a healthy skin function as the observed delayed wound healing can contribute to increased risks of infection. To counteract delayed wound healing in space, a better understanding of the fibroblasts' reaction to altered gravity levels is needed. In this paper, we describe experiments that were carried out at the Large Diameter Centrifuge located in ESA-ESTEC as part of the ESA Academy 2021 Spin Your Thesis! Campaign. We exposed dermal fibroblasts to a set of altered gravity levels, including transitions between simulated microgravity and hypergravity. The addition of the stress hormone cortisol to the cell culture medium was done to account for possible interaction effects of gravity and cortisol exposure. Results show a main impact of cortisol on the secretion of pro-inflammatory cytokines as well as extracellular matrix proteins. Altered gravity mostly induced a delay in cellular migration and changes in mechanosensitive cell structures. Furthermore, 20 x g hypergravity transitions induced changes in nuclear morphology. These findings provide insights into the effect of gravity transitions on the fibroblasts' function related to wound healing, which may be useful for the development of countermeasures.}},
  articleno    = {{48}},
  author       = {{Radstake, Wilhelmina and Gautam, Kiran and Ferreira Da Silva Miranda, Silvana and Van Rompay, Cynthia and Vermeesen, Randy and Tabury, Kevin and Verslegers, Mieke and Dowson, Alan and Gorissen, Jeffrey and van Loon, Jack J. W. A. and Savage, Nigel D. L. and Baatout, Sarah and Baselet, Bjorn}},
  issn         = {{2373-8065}},
  journal      = {{NPJ MICROGRAVITY}},
  keywords     = {{Space and Planetary Science,Physics and Astronomy (miscellaneous),Agricultural and Biological Sciences (miscellaneous),Biochemistry, Genetics and Molecular Biology (miscellaneous),Materials Science (miscellaneous),Medicine (miscellaneous)}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{11}},
  publisher    = {{Springer Science and Business Media LLC}},
  title        = {{Gravitational effects on fibroblasts’ function in relation to wound healing}},
  url          = {{http://doi.org/10.1038/s41526-023-00286-z}},
  volume       = {{9}},
  year         = {{2023}},
}

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