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Perspectives in hydrophobic interaction temperature-responsive liquid chromatography (TRLC)

(2023) LCGC EUROPE. 36(2). p.63-69
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Abstract
Temperature-responsive liquid chromatography (TRLC) is an emerging green high performance liquid chromatography (HPLC) mode allowing reversed-phase-type separations while necessitating only water as the mobile phase.The columns therein are typically packed with silica particles to which stimuli-responsive polymers are anchored. In hydrophobic interaction TRLC, such polymers depict a loss of water solubility when increasing the temperature above a characteristic conversion temperature, causing large changes in retention over quite narrow and mild temperature ranges (similar to 5-55 degrees C). TRLC circumvents the concerns about analyte or column degradation that can occur when implementing high temperatures (>80 degrees C) on conventional reversed-phase columns. It allows for HPLC using only water often spiked with the additives typically used in reversed-phase LC. Therefore, this separation mode allows for greener, cheaper, and isocratic analyses under non-denaturing conditions. The absence of compositional solvent gradients also allows for the exploitation of temperature gradients in combination with refractive index detection. Purely aqueous hydrophobic interaction TRLC is mostly applicable for solutes depicting a 1 < LogP < 5, yet these ranges can be expanded through implementation of combined aqueous or organic mobiles phases, while preserving the temperature-responsive effects. In this first TRLC instalment, our recent developments, new possibilities, and current limitations of the use of 1D TRLC are discussed, while the column performance is described with respect to the fundamentals of HPLC.
Keywords
PHASE OPTIMIZED SELECTIVITY, N-ISOPROPYLACRYLAMIDE, LC

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Citation

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MLA
Lynen, Frederic, et al. “Perspectives in Hydrophobic Interaction Temperature-Responsive Liquid Chromatography (TRLC).” LCGC EUROPE, vol. 36, no. 2, 2023, pp. 63–69.
APA
Lynen, F., Ampe, A., Bandini, E., Baert, M., Wicht, K., Kajtazi, A., … Spileers, G. (2023). Perspectives in hydrophobic interaction temperature-responsive liquid chromatography (TRLC). LCGC EUROPE, 36(2), 63–69.
Chicago author-date
Lynen, Frederic, Adriaan Ampe, Elena Bandini, Mathijs Baert, Kristina Wicht, Ardiana Kajtazi, Turaj Rahmani, Jonas Veenhoven, and Gaëlle Spileers. 2023. “Perspectives in Hydrophobic Interaction Temperature-Responsive Liquid Chromatography (TRLC).” LCGC EUROPE 36 (2): 63–69.
Chicago author-date (all authors)
Lynen, Frederic, Adriaan Ampe, Elena Bandini, Mathijs Baert, Kristina Wicht, Ardiana Kajtazi, Turaj Rahmani, Jonas Veenhoven, and Gaëlle Spileers. 2023. “Perspectives in Hydrophobic Interaction Temperature-Responsive Liquid Chromatography (TRLC).” LCGC EUROPE 36 (2): 63–69.
Vancouver
1.
Lynen F, Ampe A, Bandini E, Baert M, Wicht K, Kajtazi A, et al. Perspectives in hydrophobic interaction temperature-responsive liquid chromatography (TRLC). LCGC EUROPE. 2023;36(2):63–9.
IEEE
[1]
F. Lynen et al., “Perspectives in hydrophobic interaction temperature-responsive liquid chromatography (TRLC),” LCGC EUROPE, vol. 36, no. 2, pp. 63–69, 2023.
@article{01H42ZRZ4P15E6TRVJFN1SVNYM,
  abstract     = {{Temperature-responsive liquid chromatography (TRLC) is an emerging green high performance liquid chromatography (HPLC) mode allowing reversed-phase-type separations while necessitating only water as the mobile phase.The columns therein are typically packed with silica particles to which stimuli-responsive polymers are anchored. In hydrophobic interaction TRLC, such polymers depict a loss of water solubility when increasing the temperature above a characteristic conversion temperature, causing large changes in retention over quite narrow and mild temperature ranges (similar to 5-55 degrees C). TRLC circumvents the concerns about analyte or column degradation that can occur when implementing high temperatures (>80 degrees C) on conventional reversed-phase columns. It allows for HPLC using only water often spiked with the additives typically used in reversed-phase LC. Therefore, this separation mode allows for greener, cheaper, and isocratic analyses under non-denaturing conditions. The absence of compositional solvent gradients also allows for the exploitation of temperature gradients in combination with refractive index detection. Purely aqueous hydrophobic interaction TRLC is mostly applicable for solutes depicting a 1 < LogP < 5, yet these ranges can be expanded through implementation of combined aqueous or organic mobiles phases, while preserving the temperature-responsive effects. In this first TRLC instalment, our recent developments, new possibilities, and current limitations of the use of 1D TRLC are discussed, while the column performance is described with respect to the fundamentals of HPLC.}},
  author       = {{Lynen, Frederic and Ampe, Adriaan and Bandini, Elena and Baert, Mathijs and Wicht, Kristina and Kajtazi, Ardiana and Rahmani, Turaj and Veenhoven, Jonas and Spileers, Gaëlle}},
  issn         = {{1471-6577}},
  journal      = {{LCGC EUROPE}},
  keywords     = {{PHASE OPTIMIZED SELECTIVITY,N-ISOPROPYLACRYLAMIDE,LC}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{63--69}},
  title        = {{Perspectives in hydrophobic interaction temperature-responsive liquid chromatography (TRLC)}},
  volume       = {{36}},
  year         = {{2023}},
}

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