
Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina
- Author
- Gowri Amanda Hettiarachchi (UGent) , Margo Cnockaert (UGent) , Marie Joossens (UGent) , David Laureys (UGent) , Jessika De Clippeleer (UGent) , Nicolas J. Vereecken, Denis Michez, Guy Smagghe (UGent) , Dirk de Graaf (UGent) and Peter Vandamme (UGent)
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- Abstract
- We provide a culturomics analysis of the cultivable bacterial communities of the crop, midgut and hindgut compartments, as well as the ovaries, of the invasive insect Vespa velutina, along with a cultivation-independent analysis of samples of the same nest through 16S rRNA amplicon sequencing. The Vespa velutina bacterial symbiont community was dominated by the genera Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma. Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core lactic acid bacteria (LAB) symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core LAB symbionts with strongly reduced genome sizes. Sphingomonas and Spiroplasma were the only non-LAB core symbionts but were not isolated. Convivina bacteria were particularly enriched in the hornet crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. which was adapted towards carbohydrate metabolism.
- Keywords
- Convivina, culturomics, gut symbiont, invasive insect, Vespa velutina, LACTIC-ACID BACTERIA, INTESTINI GEN. NOV., MICROBIAL COMMUNITIES, HONEY-BEES, DIVERSITY, IDENTIFICATION, GENOMICS, DATABASE, IMPACTS, QUALITY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01H3XTAPQG22ARCK3E9QJHCV6M
- MLA
- Hettiarachchi, Gowri Amanda, et al. “Convivina Is a Specialised Core Gut Symbiont of the Invasive Hornet Vespa Velutina.” INSECT MOLECULAR BIOLOGY, vol. 32, no. 5, 2023, pp. 510–27, doi:10.1111/imb.12847.
- APA
- Hettiarachchi, G. A., Cnockaert, M., Joossens, M., Laureys, D., De Clippeleer, J., Vereecken, N. J., … Vandamme, P. (2023). Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina. INSECT MOLECULAR BIOLOGY, 32(5), 510–527. https://doi.org/10.1111/imb.12847
- Chicago author-date
- Hettiarachchi, Gowri Amanda, Margo Cnockaert, Marie Joossens, David Laureys, Jessika De Clippeleer, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, Dirk de Graaf, and Peter Vandamme. 2023. “Convivina Is a Specialised Core Gut Symbiont of the Invasive Hornet Vespa Velutina.” INSECT MOLECULAR BIOLOGY 32 (5): 510–27. https://doi.org/10.1111/imb.12847.
- Chicago author-date (all authors)
- Hettiarachchi, Gowri Amanda, Margo Cnockaert, Marie Joossens, David Laureys, Jessika De Clippeleer, Nicolas J. Vereecken, Denis Michez, Guy Smagghe, Dirk de Graaf, and Peter Vandamme. 2023. “Convivina Is a Specialised Core Gut Symbiont of the Invasive Hornet Vespa Velutina.” INSECT MOLECULAR BIOLOGY 32 (5): 510–527. doi:10.1111/imb.12847.
- Vancouver
- 1.Hettiarachchi GA, Cnockaert M, Joossens M, Laureys D, De Clippeleer J, Vereecken NJ, et al. Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina. INSECT MOLECULAR BIOLOGY. 2023;32(5):510–27.
- IEEE
- [1]G. A. Hettiarachchi et al., “Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina,” INSECT MOLECULAR BIOLOGY, vol. 32, no. 5, pp. 510–527, 2023.
@article{01H3XTAPQG22ARCK3E9QJHCV6M, abstract = {{We provide a culturomics analysis of the cultivable bacterial communities of the crop, midgut and hindgut compartments, as well as the ovaries, of the invasive insect Vespa velutina, along with a cultivation-independent analysis of samples of the same nest through 16S rRNA amplicon sequencing. The Vespa velutina bacterial symbiont community was dominated by the genera Convivina, Fructobacillus, Lactiplantibacillus, Lactococcus, Sphingomonas and Spiroplasma. Lactococcus lactis and Lactiplantibacillus plantarum represented generalist core lactic acid bacteria (LAB) symbionts, while Convivina species and Fructobacillus fructosus represented highly specialised core LAB symbionts with strongly reduced genome sizes. Sphingomonas and Spiroplasma were the only non-LAB core symbionts but were not isolated. Convivina bacteria were particularly enriched in the hornet crop and included Convivina intestini, a species adapted towards amino acid metabolism, and Convivina praedatoris sp. nov. which was adapted towards carbohydrate metabolism.}}, author = {{Hettiarachchi, Gowri Amanda and Cnockaert, Margo and Joossens, Marie and Laureys, David and De Clippeleer, Jessika and Vereecken, Nicolas J. and Michez, Denis and Smagghe, Guy and de Graaf, Dirk and Vandamme, Peter}}, issn = {{0962-1075}}, journal = {{INSECT MOLECULAR BIOLOGY}}, keywords = {{Convivina,culturomics,gut symbiont,invasive insect,Vespa velutina,LACTIC-ACID BACTERIA,INTESTINI GEN. NOV.,MICROBIAL COMMUNITIES,HONEY-BEES,DIVERSITY,IDENTIFICATION,GENOMICS,DATABASE,IMPACTS,QUALITY}}, language = {{eng}}, number = {{5}}, pages = {{510--527}}, title = {{Convivina is a specialised core gut symbiont of the invasive hornet Vespa velutina}}, url = {{http://doi.org/10.1111/imb.12847}}, volume = {{32}}, year = {{2023}}, }
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