Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS
- Author
- Margot De Spiegeleer (UGent) , Vera Plekhova (UGent) , Jozefien Geltmeyer (UGent) , Ella Schoolaert (UGent) , Beata Pomian (UGent) , Varoon Singh, Kathleen Wijnant (UGent) , Kimberly De Windt (UGent) , Volter Paukku (UGent) , Alexander De Loof, Inge Gies, Nathalie Michels (UGent) , Stefaan De Henauw (UGent) , Marilyn De Graeve (UGent) , Karen De Clerck (UGent) and Lynn Vanhaecke (UGent)
- Organization
- Project
-
- Rectal sampler for metabotyping of overweight and obese children towards early prevention and prognosis of metabolic diseases
- Salivary metabolomics as a new tool for unraveling stress obesity pathways in adolescents from a clinical public health perspective
- Rectal sampling and ambient ionization based metabolomics for point-of-care precision medicine in children.
- Emotion Regulation in the Prevention and Treatment of Obesity in Young Adolescents
- Metabolic fingerprinting towards diagnosis and prognosis of cow's milk allergy in children.
- Rapid Evaporative Ionisation Mass Spectrometry for metabolomics in health and disease
- Novel sampler for ambient ionization high-resolution mass spectrometry (AIMS)-based metabolomics and its application in pediatrics precision medicine.
- Abstract
- In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification.
- Keywords
- Multidisciplinary, ASSISTED LASER DESORPTION/IONIZATION, IONIZATION MASS-SPECTROMETRY, FECAL METABOLOME, ION DESORPTION, CHILDREN, WEIGHT, HUMANS, INDEX
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01H2WM9ZBP36BZ136NHBHN8D4N
- MLA
- De Spiegeleer, Margot, et al. “Point-of-Care Applicable Metabotyping Using Biofluid-Specific Electrospun MetaSAMPs Directly Amenable to Ambient LA-REIMS.” SCIENCE ADVANCES, vol. 9, no. 23, American Association for the Advancement of Science (AAAS), 2023, doi:10.1126/sciadv.ade9933.
- APA
- De Spiegeleer, M., Plekhova, V., Geltmeyer, J., Schoolaert, E., Pomian, B., Singh, V., … Vanhaecke, L. (2023). Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS. SCIENCE ADVANCES, 9(23). https://doi.org/10.1126/sciadv.ade9933
- Chicago author-date
- De Spiegeleer, Margot, Vera Plekhova, Jozefien Geltmeyer, Ella Schoolaert, Beata Pomian, Varoon Singh, Kathleen Wijnant, et al. 2023. “Point-of-Care Applicable Metabotyping Using Biofluid-Specific Electrospun MetaSAMPs Directly Amenable to Ambient LA-REIMS.” SCIENCE ADVANCES 9 (23). https://doi.org/10.1126/sciadv.ade9933.
- Chicago author-date (all authors)
- De Spiegeleer, Margot, Vera Plekhova, Jozefien Geltmeyer, Ella Schoolaert, Beata Pomian, Varoon Singh, Kathleen Wijnant, Kimberly De Windt, Volter Paukku, Alexander De Loof, Inge Gies, Nathalie Michels, Stefaan De Henauw, Marilyn De Graeve, Karen De Clerck, and Lynn Vanhaecke. 2023. “Point-of-Care Applicable Metabotyping Using Biofluid-Specific Electrospun MetaSAMPs Directly Amenable to Ambient LA-REIMS.” SCIENCE ADVANCES 9 (23). doi:10.1126/sciadv.ade9933.
- Vancouver
- 1.De Spiegeleer M, Plekhova V, Geltmeyer J, Schoolaert E, Pomian B, Singh V, et al. Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS. SCIENCE ADVANCES. 2023;9(23).
- IEEE
- [1]M. De Spiegeleer et al., “Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS,” SCIENCE ADVANCES, vol. 9, no. 23, 2023.
@article{01H2WM9ZBP36BZ136NHBHN8D4N,
abstract = {{
In recent years, ambient ionization mass spectrometry (AIMS) including laser ablation rapid evaporation IMS, has enabled direct biofluid metabolome analysis. AIMS procedures are, however, still hampered by both analytical, i.e., matrix effects, and practical, i.e., sample transport stability, drawbacks that impede metabolome coverage. In this study, we aimed at developing biofluid-specific metabolome sampling membranes (MetaSAMPs) that offer a directly applicable and stabilizing substrate for AIMS. Customized rectal, salivary, and urinary MetaSAMPs consisting of electrospun (nano)fibrous membranes of blended hydrophilic (polyvinylpyrrolidone and polyacrylonitrile) and lipophilic (polystyrene) polymers supported metabolite absorption, adsorption, and desorption. Moreover, MetaSAMP demonstrated superior metabolome coverage and transport stability compared to crude biofluid analysis and was successfully validated in two pediatric cohorts (MetaBEAse, n = 234 and OPERA, n = 101). By integrating anthropometric and (patho)physiological with MetaSAMP-AIMS metabolome data, we obtained substantial weight-driven predictions and clinical correlations. In conclusion, MetaSAMP holds great clinical application potential for on-the-spot metabolic health stratification.}},
author = {{De Spiegeleer, Margot and Plekhova, Vera and Geltmeyer, Jozefien and Schoolaert, Ella and Pomian, Beata and Singh, Varoon and Wijnant, Kathleen and De Windt, Kimberly and Paukku, Volter and De Loof, Alexander and Gies, Inge and Michels, Nathalie and De Henauw, Stefaan and De Graeve, Marilyn and De Clerck, Karen and Vanhaecke, Lynn}},
issn = {{2375-2548}},
journal = {{SCIENCE ADVANCES}},
keywords = {{Multidisciplinary,ASSISTED LASER DESORPTION/IONIZATION,IONIZATION MASS-SPECTROMETRY,FECAL METABOLOME,ION DESORPTION,CHILDREN,WEIGHT,HUMANS,INDEX}},
language = {{eng}},
number = {{23}},
pages = {{18}},
publisher = {{American Association for the Advancement of Science (AAAS)}},
title = {{Point-of-care applicable metabotyping using biofluid-specific electrospun MetaSAMPs directly amenable to ambient LA-REIMS}},
url = {{http://doi.org/10.1126/sciadv.ade9933}},
volume = {{9}},
year = {{2023}},
}
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