
Cefquinome shows a higher impact on the pig gut microbiome and resistome compared to ceftiofur
- Author
- Sofie Rutjens (UGent) , Nick Vereecke (UGent) , Jannes Sauer (UGent) , Siska Croubels (UGent) and Mathias Devreese (UGent)
- Organization
- Project
- Abstract
- Cephalosporins are licensed for treatment of severe bacterial infections in different species. However, the effect of these antimicrobials on the fecal microbiome and potential spread of resistance-associated genes causes great concern. This highlights the need to understand the impact of cephalosporins on the porcine fecal microbiome and resistome. A combination of long-read 16S rRNA gene and shotgun metagenomic sequencing was applied to investigate the effect of conventional treatment with either ceftiofur (3 mg.kg(-1) intramuscular, 3 consecutive days) or cefquinome (2 mg.kg(-1) intramuscular, 5 consecutive days) on the porcine microbiome and resistome. Fecal samples were collected from 17 pigs (6 ceftiofur treated, 6 cefquinome treated, 5 control pigs) at four different timepoints. Treatment with ceftiofur resulted in an increase in Proteobacteria members on microbiome level, while on resistome level selection in TetQ containing Bacteroides, CfxA6 containing Prevotella and bla(TEM-1) containing Escherichia coli was observed. Cefquinome treatment resulted in a decline in overall species richness (alpha-diversity) and increase in Proteobacteria members. On genus level, administration of cefquinome significantly affected more genera than ceftiofur (18 vs 8). On resistome level, cefquinome resulted in a significant increase of six antimicrobial resistance genes, with no clear correlation with certain genera. For both antimicrobials, the resistome levels returned back to the control levels 21 days post-treatment. Overall, our study provides novel insights on the effect of specific cephalosporins on the porcine gut microbiome and resistome after conventional intramuscular treatment. These results might contribute to better tailoring of the most ideal treatment strategy for some bacterial infections.
- Keywords
- General Veterinary, Veterinary cephalosporins, gut microbiome, resistome, ARGs, porcine feces, 16S rRNA sequencing, shotgun sequencing, metagenomics, 4TH GENERATION CEPHALOSPORINS, ESCHERICHIA-COLI, ANTIMICROBIAL RESISTANCE, SPECTRUM CEPHALOSPORIN, ANTIBIOTIC-RESISTANCE, FIRMICUTES, BACTERIA, ANIMALS, FARMS, 3RD
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-01H2SPAZCME97H2JTCNPR8V7HW
- MLA
- Rutjens, Sofie, et al. “Cefquinome Shows a Higher Impact on the Pig Gut Microbiome and Resistome Compared to Ceftiofur.” VETERINARY RESEARCH, vol. 54, no. 1, BMC, 2023, doi:10.1186/s13567-023-01176-8.
- APA
- Rutjens, S., Vereecke, N., Sauer, J., Croubels, S., & Devreese, M. (2023). Cefquinome shows a higher impact on the pig gut microbiome and resistome compared to ceftiofur. VETERINARY RESEARCH, 54(1). https://doi.org/10.1186/s13567-023-01176-8
- Chicago author-date
- Rutjens, Sofie, Nick Vereecke, Jannes Sauer, Siska Croubels, and Mathias Devreese. 2023. “Cefquinome Shows a Higher Impact on the Pig Gut Microbiome and Resistome Compared to Ceftiofur.” VETERINARY RESEARCH 54 (1). https://doi.org/10.1186/s13567-023-01176-8.
- Chicago author-date (all authors)
- Rutjens, Sofie, Nick Vereecke, Jannes Sauer, Siska Croubels, and Mathias Devreese. 2023. “Cefquinome Shows a Higher Impact on the Pig Gut Microbiome and Resistome Compared to Ceftiofur.” VETERINARY RESEARCH 54 (1). doi:10.1186/s13567-023-01176-8.
- Vancouver
- 1.Rutjens S, Vereecke N, Sauer J, Croubels S, Devreese M. Cefquinome shows a higher impact on the pig gut microbiome and resistome compared to ceftiofur. VETERINARY RESEARCH. 2023;54(1).
- IEEE
- [1]S. Rutjens, N. Vereecke, J. Sauer, S. Croubels, and M. Devreese, “Cefquinome shows a higher impact on the pig gut microbiome and resistome compared to ceftiofur,” VETERINARY RESEARCH, vol. 54, no. 1, 2023.
@article{01H2SPAZCME97H2JTCNPR8V7HW, abstract = {{Cephalosporins are licensed for treatment of severe bacterial infections in different species. However, the effect of these antimicrobials on the fecal microbiome and potential spread of resistance-associated genes causes great concern. This highlights the need to understand the impact of cephalosporins on the porcine fecal microbiome and resistome. A combination of long-read 16S rRNA gene and shotgun metagenomic sequencing was applied to investigate the effect of conventional treatment with either ceftiofur (3 mg.kg(-1) intramuscular, 3 consecutive days) or cefquinome (2 mg.kg(-1) intramuscular, 5 consecutive days) on the porcine microbiome and resistome. Fecal samples were collected from 17 pigs (6 ceftiofur treated, 6 cefquinome treated, 5 control pigs) at four different timepoints. Treatment with ceftiofur resulted in an increase in Proteobacteria members on microbiome level, while on resistome level selection in TetQ containing Bacteroides, CfxA6 containing Prevotella and bla(TEM-1) containing Escherichia coli was observed. Cefquinome treatment resulted in a decline in overall species richness (alpha-diversity) and increase in Proteobacteria members. On genus level, administration of cefquinome significantly affected more genera than ceftiofur (18 vs 8). On resistome level, cefquinome resulted in a significant increase of six antimicrobial resistance genes, with no clear correlation with certain genera. For both antimicrobials, the resistome levels returned back to the control levels 21 days post-treatment. Overall, our study provides novel insights on the effect of specific cephalosporins on the porcine gut microbiome and resistome after conventional intramuscular treatment. These results might contribute to better tailoring of the most ideal treatment strategy for some bacterial infections.}}, articleno = {{45}}, author = {{Rutjens, Sofie and Vereecke, Nick and Sauer, Jannes and Croubels, Siska and Devreese, Mathias}}, issn = {{0928-4249}}, journal = {{VETERINARY RESEARCH}}, keywords = {{General Veterinary,Veterinary cephalosporins,gut microbiome,resistome,ARGs,porcine feces,16S rRNA sequencing,shotgun sequencing,metagenomics,4TH GENERATION CEPHALOSPORINS,ESCHERICHIA-COLI,ANTIMICROBIAL RESISTANCE,SPECTRUM CEPHALOSPORIN,ANTIBIOTIC-RESISTANCE,FIRMICUTES,BACTERIA,ANIMALS,FARMS,3RD}}, language = {{eng}}, number = {{1}}, pages = {{17}}, publisher = {{BMC}}, title = {{Cefquinome shows a higher impact on the pig gut microbiome and resistome compared to ceftiofur}}, url = {{http://doi.org/10.1186/s13567-023-01176-8}}, volume = {{54}}, year = {{2023}}, }
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