Academic Bibliography

 Search 200 years of publications by Ghent University researchers.
Advanced search
1 file | 6.72 MB
Add to list
  1. Search results
  2. Mapping the adaptor protein complex i...

Mapping the adaptor protein complex interaction network in Arabidopsis identifies P34 as a common stability regulator

Peng Wang (UGent) , Wei Siao (UGent) , Xiuyang Zhao, Deepanksha Arora, Ren Wang (UGent) , Dominique Eeckhout (UGent) , Jelle Van Leene (UGent) , Rahul Kumar (UGent) , Anaxi Houbaert, Nancy De Winne (UGent) , et al.
(2022) BIORXIV. p.1-55
Author
Organization
Project
Abstract
Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargos and coated vesicle accessory proteins. Since in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargos of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins, and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells.
Keywords
Arabidopsis, protein complex, adaptor protein

Downloads

  • Download
    2022.08.31.505729v1.full.pdf
    • full text (Author's original)
    • |
    • open access
    • |
    • PDF
    • |
    • 6.72 MB

Citation

Please use this url to cite or link to this publication:

MLA
Wang, Peng, et al. “Mapping the Adaptor Protein Complex Interaction Network in Arabidopsis Identifies P34 as a Common Stability Regulator.” BIORXIV, 2022, pp. 1–55, doi:10.1101/2022.08.31.505729.
APA
Wang, P., Siao, W., Zhao, X., Arora, D., Wang, R., Eeckhout, D., … Russinova, E. (2022). Mapping the adaptor protein complex interaction network in Arabidopsis identifies P34 as a common stability regulator. https://doi.org/10.1101/2022.08.31.505729
Chicago author-date
Wang, Peng, Wei Siao, Xiuyang Zhao, Deepanksha Arora, Ren Wang, Dominique Eeckhout, Jelle Van Leene, et al. 2022. “Mapping the Adaptor Protein Complex Interaction Network in Arabidopsis Identifies P34 as a Common Stability Regulator.” BIORXIV. https://doi.org/10.1101/2022.08.31.505729.
Chicago author-date (all authors)
Wang, Peng, Wei Siao, Xiuyang Zhao, Deepanksha Arora, Ren Wang, Dominique Eeckhout, Jelle Van Leene, Rahul Kumar, Anaxi Houbaert, Nancy De Winne, Evelien Mylle, Michaël Vandorpe, R. A. Korver, C. Testerink, Kris Gevaert, Steffen Vanneste, Geert De Jaeger, Daniël Van Damme, and Eugenia Russinova. 2022. “Mapping the Adaptor Protein Complex Interaction Network in Arabidopsis Identifies P34 as a Common Stability Regulator.” BIORXIV. doi:10.1101/2022.08.31.505729.
Vancouver
1.
Wang P, Siao W, Zhao X, Arora D, Wang R, Eeckhout D, et al. Mapping the adaptor protein complex interaction network in Arabidopsis identifies P34 as a common stability regulator. BIORXIV. 2022. p. 1–55.
IEEE
[1]
P. Wang et al., “Mapping the adaptor protein complex interaction network in Arabidopsis identifies P34 as a common stability regulator,” BIORXIV. pp. 1–55, 2022.
@misc{01H2QVTNHFA4D5GA5RJ8QQD6MQ,
  abstract     = {{Adaptor protein (AP) complexes are evolutionarily conserved vesicle transport regulators that recruit coat proteins, membrane cargos and coated vesicle accessory proteins. Since in plants endocytic and post-Golgi trafficking intersect at the trans-Golgi network, unique mechanisms for sorting cargos of overlapping vesicular routes are anticipated. The plant AP complexes are part of the sorting machinery, but despite some functional information, their cargoes, accessory proteins, and regulation remain largely unknown. Here, by means of various proteomics approaches, we generated the overall interactome of the five AP and the TPLATE complexes in Arabidopsis thaliana. The interactome converged on a number of hub proteins, including the thus far unknown adaptin binding-like protein, designated P34. P34 interacted with the clathrin-associated AP complexes, controlled their stability and, subsequently, influenced clathrin-mediated endocytosis and various post-Golgi trafficking routes. Altogether, the AP interactome network offers substantial resources for further discoveries of unknown endomembrane trafficking regulators in plant cells.}},
  author       = {{Wang, Peng and Siao, Wei and Zhao, Xiuyang and Arora, Deepanksha and Wang, Ren and Eeckhout, Dominique and Van Leene, Jelle and Kumar, Rahul and Houbaert, Anaxi and De Winne, Nancy and Mylle, Evelien and Vandorpe, Michaël and  Korver, R. A. and  Testerink, C. and Gevaert, Kris and Vanneste, Steffen and De Jaeger, Geert and Van Damme, Daniël and Russinova, Eugenia}},
  keywords     = {{Arabidopsis,protein complex,adaptor protein}},
  language     = {{eng}},
  pages        = {{1--55}},
  series       = {{BIORXIV}},
  title        = {{Mapping the adaptor protein complex interaction network in Arabidopsis identifies P34 as a common stability regulator}},
  url          = {{http://doi.org/10.1101/2022.08.31.505729}},
  year         = {{2022}},
}
Altmetric
View in Altmetric
Web of Science
Times cited: