@article{01H1KDBC0ZAK5C9DTG14J3AHK2,
  abstract     = {{The  morphological  and  molecular  characterisations  of  two  lance  nematode   species   isolated   from   the   rhizosphere   of   banana,   Hoplolaimus  seinhorsti  and  H.  pararobustus,  are  provided  based  on  an  integrative  study  that  includes  light  and  scanning  electron  microscopy,  phylogenetic  analysis  and  two  tree-based  molecular  species  delimitation  methods  (GMYC  and  bPTP).  Nineteen  new  sequences  were  obtained,  including  5  partial  18S  rRNA,  6  D2-D3  of  28S  rRNA,  1  ITS  rRNA  and  7  COI  mtDNA  (the  first  COI sequences  of  H.  seinhorsti  and  H.  pararobustus),  and  an  updated  morphological  character  comparison  of  37  Hoplolaimus  species  is    presented.    The    tree-based    molecular    species-delimitation    approaches  employed  gave  markedly  differing  results,  and  also  showed  remarkable  discrepancies  among  the  investigated  genes,  although the bPTP output was found to agree well with established morphological   species   delimitations.   Both   species-delimitation   approaches  did,  however,  provide  the  same  output  for  the  COI mtDNA  sequences,  and  the  COI  mtDNA  gene  sequence  was  also  found to correspond better to established morphological species. It is  therefore  recommended  by  this  paper  as  representing  the  most  suitable barcode marker for Hoplolaimus species identification. This integrative  study  also  resulted  in  the  corrective  reassignment  of  17  gene  sequences  that  were  previously  unidentified  or  incorrectly  classified, as well as concluding that H. pararobustus consists of two cryptic species.}},
  author       = {{Olajide, Emmanuel Olorunfemi and Singh, Phougeishangbam Rolish and Kolombia, Yao A. and Rumbarar, Merlin Kornelia and Couvreur, Marjolein and Bert, Wim}},
  editor       = {{Castillo, Pablo}},
  issn         = {{0022-300X}},
  journal      = {{JOURNAL OF NEMATOLOGY}},
  keywords     = {{28S rDNA,ITS rDNA,COI,Banana,Hoplolaimus,Lance nematode,Morphology,Phylogeny,Plant-parasitic nematode,Scanning electron microscopy,Systematic,Taxonomy,PLANT-PARASITIC NEMATODES,INTERNAL TRANSCRIBED SPACER,LANCE NEMATODE,MOLECULAR CHARACTERIZATION,UNITED-STATES,SPP.,DELIMITATION,FIELDS,COTTON,RDNA}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{17--24}},
  title        = {{Characterization of Hoplolaimus seinhorsti and Hoplolaimus pararobustus (Tylenchina : Hoplolaimidae) from banana, with phylogeny and species delineation in the genus Hoplolaimus}},
  url          = {{http://doi.org/10.2478/jofnem-2023-0019}},
  volume       = {{55}},
  year         = {{2023}},
}

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