Advanced search
1 file | 1.18 MB Add to list

A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms

Annelies Bogaert (UGent) , Tessa Van de Steene (UGent) , Marnik Vuylsteke (UGent) , Sven Eyckerman (UGent) and Kris Gevaert (UGent)
Author
Organization
Abstract
Given that up to 20% of N-termini of human proteins differ from canonical N-termini as retrieved from sequence databases, a variety of N-terminal proteoforms exists in human cells. These N-terminal proteoforms arise through alternative translation initiation or alternative splicing among others. While such proteoforms diversify the biological functions of the proteome, they remain largely understudied. Recent studies showed that proteoforms expand protein interaction networks by interacting with different prey proteins. As a mass spectrometry-based method to study protein-protein interactions, Virotrap avoids cell lysis by trapping protein complexes in viral-like particles, thereby allowing for the identification of transient and less stable interactions. This chapter describes an adjusted version of Virotrap, decoupled Virotrap, that allows for the detection of interaction partners specific for N-terminal proteoforms.
Keywords
Protein-protein interactions, N-terminal proteoforms, Virotrap, Proteome complexity, Mass spectrometry

Downloads

  • (...).pdf
    • full text (Published version)
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.18 MB

Citation

Please use this url to cite or link to this publication:

MLA
Bogaert, Annelies, et al. “A Decoupled Virotrap Approach to Study the Interactomes of N-Terminal Proteoforms.” Methods in Enzymology, edited by Thomas Arnesen, vol. 684, Academic Press, 2023, pp. 253–87, doi:10.1016/bs.mie.2023.02.003.
APA
Bogaert, A., Van de Steene, T., Vuylsteke, M., Eyckerman, S., & Gevaert, K. (2023). A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms. In T. Arnesen (Ed.), Methods in enzymology (Vol. 684, pp. 253–287). https://doi.org/10.1016/bs.mie.2023.02.003
Chicago author-date
Bogaert, Annelies, Tessa Van de Steene, Marnik Vuylsteke, Sven Eyckerman, and Kris Gevaert. 2023. “A Decoupled Virotrap Approach to Study the Interactomes of N-Terminal Proteoforms.” In Methods in Enzymology, edited by Thomas Arnesen, 684:253–87. Academic Press. https://doi.org/10.1016/bs.mie.2023.02.003.
Chicago author-date (all authors)
Bogaert, Annelies, Tessa Van de Steene, Marnik Vuylsteke, Sven Eyckerman, and Kris Gevaert. 2023. “A Decoupled Virotrap Approach to Study the Interactomes of N-Terminal Proteoforms.” In Methods in Enzymology, ed by. Thomas Arnesen, 684:253–287. Academic Press. doi:10.1016/bs.mie.2023.02.003.
Vancouver
1.
Bogaert A, Van de Steene T, Vuylsteke M, Eyckerman S, Gevaert K. A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms. In: Arnesen T, editor. Methods in enzymology. Academic Press; 2023. p. 253–87.
IEEE
[1]
A. Bogaert, T. Van de Steene, M. Vuylsteke, S. Eyckerman, and K. Gevaert, “A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms,” in Methods in enzymology, vol. 684, T. Arnesen, Ed. Academic Press, 2023, pp. 253–287.
@incollection{01H1BC1JMX0FF4PG2R6NW6AXW9,
  abstract     = {{Given that up to 20% of N-termini of human proteins differ from canonical N-termini as retrieved from sequence databases, a variety of N-terminal proteoforms exists in human cells. These N-terminal proteoforms arise through alternative translation initiation or alternative splicing among others. While such proteoforms diversify the biological functions of the proteome, they remain largely understudied. Recent studies showed that proteoforms expand protein interaction networks by interacting with different prey proteins. As a mass spectrometry-based method to study protein-protein interactions, Virotrap avoids cell lysis by trapping protein complexes in viral-like particles, thereby allowing for the identification of transient and less stable interactions. This chapter describes an adjusted version of Virotrap, decoupled Virotrap, that allows for the detection of interaction partners specific for N-terminal proteoforms.}},
  author       = {{Bogaert, Annelies and Van de Steene, Tessa and Vuylsteke, Marnik and Eyckerman, Sven and Gevaert, Kris}},
  booktitle    = {{Methods in enzymology}},
  editor       = {{Arnesen, Thomas}},
  isbn         = {{9780443157721}},
  issn         = {{0076-6879}},
  keywords     = {{Protein-protein interactions,N-terminal proteoforms,Virotrap,Proteome complexity,Mass spectrometry}},
  language     = {{eng}},
  pages        = {{253--287}},
  publisher    = {{Academic Press}},
  series       = {{Modifications and targeting of protein termini : part A}},
  title        = {{A decoupled Virotrap approach to study the interactomes of N-terminal proteoforms}},
  url          = {{http://doi.org/10.1016/bs.mie.2023.02.003}},
  volume       = {{684}},
  year         = {{2023}},
}

Altmetric
View in Altmetric